Interferon-, mouse (mIFN-) 11276905001

IFN-γ (interferon-γ) is produced by natural killer T cells, macrophages, Th1 CD4 T cells and activated CD8 T cells and the production increases with age.
Recombinant, Interferon-, mouse (mlFN-) is produced in E. coli and purified by standard chromatographic techniques.
Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity.
Specificity
Species specificity: mouse
Interferon-γ, mouse (mIFN-γ) has been used in cell culture.
Recombinant mouse interferon-γ is a valuable tool for the study of IFN-γ (interferon-γ) actions in mouse model systems.
Biochem/physiol Actions
Interferon-γ (IFNγ) participates in cell-mediated immunity and has pro-inflammatory effects on immunocytes and target tissue. Its functions vary and is tissue specific. IFNγ induces T-cell-mediated immune response by increasing MHC-I expression on target cells and acts through IFNγ receptor, which is expressed on both immune and non-immune cells. IFNγ is associated with the development of colitis and Sjogren Syndrome.
Quality

• Purity: >95% (SDS-PAGE)
• Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test)

Sequence
The primary structure of recombinant Interferon-γ, mouse (mIFN-γ) is identical to that of natural, mouse IFN-γ (one polypeptide chain, 131 amino acids), however, recombinant mIFN-γ lacks the last two amino acids at the C-terminus and is not glycosylated. Glycosylation is not essential for biological activity.
Chain Length 131 AA
Unit Definition
EC50 definition: One unit is defined as the amount of mlFN- that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN- reference standard (Gg 02-901-533). (L cells-EMC virus/cytopathic effect) (1 unit equals 0.2 ng/ml).
EC 50 definition : The amount of mIFN- that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN- reference standard (Gg 02–901-533) (L cells-EMC virus/cytopathic effect) (1 unit equals =0.2 ng/ml).
Physical form
Solution, filtered through 0.2 μm pore size membrane, 100 000 U/ml (20 μg/ml) in PBS (phosphate buffered saline) and BSA (bovine serum albumin),1 mg/ml [purity of BSA: 98%, endotoxin (LAL): < 1 EU/mg BSA].
Preparation Note
Working solution: Dilute the concentrated mlFN- solution (100,000 U/ml) with PBS or culture medium containing BSA, 1 mg/ml (0.1%) or serum, 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
For life science research only. Not for use in diagnostic procedures.
For life science research only. Not for use in diagnostic procedures.
1 EU corresponds to 0.1 ng.

Quality Level	100,
biological source	mouse
assay	>95% (SDS-PAGE)
form	solution
specific activity	>5*10^6 U/mg ((mIFN- , NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.)
packaging	pkg of 100,000 U (20 µg, 1 ml)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash