DNA Molecular Weight Marker XIII (50 bp ladder) 11721925001

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 50 to 750 bp and an additional band of 2642 bp
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests
• PCR
• RT-PCR

DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.
The fragments have 5-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
Sequence
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.
Unit Definition
50 µg = 1A260 unit
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (250 μg/ml)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash