DNA, lambda 10745782001

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5 ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Quality
The DNA shows the typical cleavage pattern for wild type DNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequence
Chain Length 48,502 bp
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Storage and Stability
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
mol wt	32,300 kDa
packaging	pkg of 1 mL
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available