DAPI 10236276001

Empirical Formula (Hill Notation):	C16H15N5 · 2HCl
CAS Number:	28718-90-3
Molecular Weight:	350.25
Beilstein/REAXYS Number:	4894417
MDL number:	MFCD00012681
PubChem Substance ID:	329748990

DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Quality
Purity: >90% (from N)
Principle
The fluorescent dye DAPI binds selectively to DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces.
Preparation Note
Working solution: Solubility: 25 mg/ml in water

Preparation of stock solution
Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.

Preparation of working solution

Dilute the stock solution with methanol to a final concentration of 1 µg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1µg/ml) at 2 to 8 °C for about 6 months.
Reconstitution
In 2 to 10 ml double-dist. water; 1 to 5 mg/ml final concentration.
Note: Prepare aliquots and store at -15 to -25 °C.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>90%
form	powder
packaging	pkg of 10 mg
Торговая марка	Roche
max	340 nm in aq. suspension
fluorescence	ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex)
shipped in	ambient
storage temp.	room temp
SMILES string	Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI	1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key	FPNZBYLXNYPRLR-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable