ABTS™ Buffer 11112597001

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA, using ABTS, is a highly sensitive, specific and reproducible technique.
ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Note
Working solution: Dissolve the contents of one bottle substrate for ABTS in 100 ml double-distilled water. This concentrate is stable when stored in aliquots at -15 to -25 °C.
To obtain the working solution, dilute one aliquot in a ratio of 1:10 with double-dist. water. Dissolve one ABTS tablet (50 mg) in 50 ml of the working solution. This solution has a light-green color. In microtiter plates the solution is colorless. A405 nm/1 cm should be < 0.16.
Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
For life science research only. Not for use in diagnostic procedures.
ABTS is a trademark of Roche

form	powder
Quality Level	100,
packaging	pkg of 16.7 g
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash