ABTS™ Buffer 11204530001

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.
ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Note
Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
For life science research only. Not for use in diagnostic procedures.
ABTS is a trademark of Roche

form	solution
Quality Level	100,
packaging	pkg of 125 mL
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available