ABTS™ 10102946001

Empirical Formula (Hill Notation):	C18H24N6O6S4
CAS Number:	30931-67-0
Molecular Weight:	548.68
Beilstein/REAXYS Number:	7329461
MDL number:	MFCD00010404
PubChem Substance ID:	329748893

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.
ABTS (C18H16N4O6S4-(NH4)2) is a peroxidase substrate for ELISA.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Quality
Purity: >98% (absorbance), chromatographically homogeneous
Preparation Note
Working concentration: ABTS should be dissolved as follows:

100 mg ABTS in in 100ml 3.25 mM sodium perborate, 39.8 mM citric acid, 60 mM disodium hydrogen phosphate, pH 4.4-4.5 (see also PI of SA-peroxidase). This buffer corresponds to the single reagent ABTS-Buffer, Cat. No. 1204530. The formed product is green and soluble in water. Measurement at 405 nm.
Dilution of ABTS:
ABTS/ buffer solution (9.1 mM ABTS; pH 5.0): 99,9 mg ABTS in 20 ml potassium phosphate buffer (0.1M; pH 5.0).
Storage and Stability
Store at 2 to 8 °C. (It is also possible to store at -15 to -25 °C)
For life science research only. Not for use in diagnostic procedures.
ABTS is a trademark of Roche

assay	>98%
Quality Level	100,
form	crystalline
mol wt	548.68
packaging	pkg of 2 g
Торговая марка	Roche
solubility	50 mg/mL
max	414 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI	1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key	OHDRQQURAXLVGJ-AXMZSLBLSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable