MISSION® 1X LacO Inducible Non-Target shRNA Control Transduction Particles SHC312V-1EA

NACRES:

NA.51

Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.
The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl--D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.
The MISSION 1x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.
To see more application data, protocols, vector maps visit sigma.com/shrna,.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Sigma is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
product line	MISSION®
concentration	1x106 VP/ml (via p24 assay)
shipped in	dry ice
storage temp.	70°C

RIDADR	UN 3245 9
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable