GenElute™ FFPE RNA/DNA Purification Plus Kit RDP200-50RXN

NACRES:

NA.52

Sigmas GenElute™ FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Sigmas GenElute™ FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

• Fast and easy processing using rapid spin-column format
• High yields and Quality of Nucleic Acids
• Separate fractionation of RNA and DNA
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions

RNA

• Bioanalyzer
• Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
• RT-PCR for both large mRNA and small RNA including miRNA
• Northern blotting
• RNase protection
• Primer extension
• Expression array assays
• Next Generation Sequencing
• microRNA Cloning
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR

Genomic DNA

• PCR and Quantitative PCR
• Sequencing
• DNA Methylation Studies
• Southern blotting
• Microarrays
• SNP analysis

• Complete column purification - Separate fractionation of RNA and DNA
• Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
• Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications

Preps
GenElute is a trademark of Sigma-Aldrich Co. LLC

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greener alternative category	Aligned
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H332 - H314 - H334 - H412
pcodes	P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310
Supp Hazards	EUH032
RIDADR	UN 3316 9
WGK Germany	WGK 3