- Производитель:
- Sigma-Aldrich
Кат. номер |
EC800-10RXN |
EC800-50RXN |
EC800-250RXN |
DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute™-E Single Spin RNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, and ethanol, from RNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required, or for RNA cleanup following silica-based isolation to remove process contaminants
GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.
With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as RT-PCR, gene expression, and NGS.
Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 µl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.
Use within 12 months of receipt.
Features and Benefits
Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality RNA: negative chromotography-based purification reduces carryover of RT-PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
purified by | (Single-spin negative chromotography), (Time: 3 minutes or less) |
feature | Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS), Intended use (For depletion of impurities from RNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) |
greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Learn more about the Principles of Green Chemistry,. |
greener alternative category | Aligned, |
storage temp. | room temp |
pictograms | GHS02 |
signalword | Danger |
pcodes | P240 - P403 + P233 |
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