Proteins & Enzymes
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Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA
eCl@ss: 42010105 NACRES: NA.83
Кат. номер P7998-100ML P7998-100ML-PW General descriptionThe product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.ApplicationDevelops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.CautionStable at least one year at 2-8 °C.Physical formReady to usePreparation NoteContains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.ПараметрыQuality Level 200 form liquid storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H315 - H318 - H373 pcodes P280 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Avidin-Peroxidase Conjugate from hen egg white/horseradish
Unit DefinitionКат. номер 11371-1MG 11371-5MG
1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5 further activity: peroxidase ≥80 U/mg [1U corresponds 1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C]
Preparation Note
Conjugate Description: ~1.5 moles peroxidase/mol avidin; MBS-linkage (Cat. No.No. 63173)Other NotesStaining immunoblots with biotinylated primary antibodiesПараметрыform powder Quality Level 100, quality lyophilized avidin activity ~5 U/mg impurities ≥80 U/mg peroxidase (further activity) color almost white storage temp. −20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BCECF/AM - CAS 117464-70-7 - Calbiochem
MDL number: MFCD00036969
Кат. номер 216254-1MG 216254-1MGCN General descriptionA variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
Packaging
1 mg in Glass bottle
Packaged under inert gas
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Irritant (B)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.Other NotesFutsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 85% (mixture of isomers, HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color white to off-white solubility acetonitrile: 25 mg/mL, DMF: soluble, DMSO: soluble fluorescence ex 500-504 nm, em 525-531 nm shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BCIP®/NBT Liquid Substrate System B1911-100ML
NACRES: NA.83 ApplicationA ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:- for staining alkaline phosphatase(ALP) in bone marrow stromal cells
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
Packaging100 mL in poly bottleCautionIt is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.Legal InformationBCIP is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form liquid storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BCIP®/NBT-Blue Liquid Substrate System for Membranes
NACRES: NA.83
Кат. номер B3804-100ML B3804-100ML-PW ApplicationA ready-to-use buffered alkaline phosphatase substrate for use in immunoblotting. Produces an insoluble dark blue-purple end product. Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Caution
Stable at least one year at 2-8 °C. This substrate is light-sensitive.Legal InformationBCIP is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100, form liquid storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BCIP®/NBT-Purple Liquid Substrate System for Membranes
NACRES: NA.83
Кат. номер B3679-100ML B3679-100ML-PW ApplicationBCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Caution
Stable at least one year at 2-8 °C.
Physical form
Ready-to-use.Legal InformationBCIP is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100, form liquid storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem
Empirical Formula (Hill Notation): C25H24N6O · 3HCl CAS Number: 23491-45-4 Molecular Weight: 533.88 MDL number: MFCD00012679
Кат. номер 382061-100MG 382061-1GM 382061-250MG 382061-1GMCN 382061-250MGCN 382061-100MGCN General descriptionMembrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065,) have been used in conjunction with RNA aptamers in the 5 untranslated region (UTR) to control expression of transfected gene constructs.
Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5 untranslated region (UTR) to control expression of transfected gene constructs.
Packaging
100, 250 mg in Plastic ampoule
1 g in Plastic ampoule
Biochem/physiol Actions
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Adenine-thymine-specific fluorescent stain
Warning
Toxicity: Carcinogenic / Teratogenic (D)
Preparation Note
Precipitates in phosphate buffered solutions
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.Other NotesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.
Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.
Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.
Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.
Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, assay 98% (HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color yellow-green fluorescence ex 346 nm, em 460 nm shipped in ambient storage temp. 2-8°C InChI 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H InChI key OKQAASMPIOSOEX-UHFFFAOYSA-N
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem
Empirical Formula (Hill Notation): C27H28N6O · 3HCl CAS Number: 23491-52-3 Molecular Weight: 561.93
Кат. номер 382065-100MG 382065-100MGCN General descriptionCell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061,) have been used in conjunction with RNA aptamers in the 5 untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
Packaging
100 mg in Alu drum
Biochem/physiol Actions
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Adenine-thymine-specific fluorescent stain
Warning
Toxicity: Toxic & Carcinogenic / Teratogenic (G)
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.Other NotesDue to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, assay 95% (TLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color yellow solubility water: 50 mg/mL fluorescence ex 346 nm, em 460 nm shipped in ambient storage temp. 2-8°C InChI 1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31) InChI key PRDFBSVERLRRMY-UHFFFAOYSA-N
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Black Gold II Myelin Staining Kit AG105
eCl@ss: 32161000 NACRES: NA.41 General descriptionBlack-Gold II is an aurohalophosphate complex which stains specifically for myelin within the central nervous system. Black-Gold II staining provides high resolution, high contrast, a short histochemical processing time, and high reproducibility. Black-Gold II is a new and improved version of its predecessor, Black-Gold (Cat # AG390). The advantages of Millipore’s Black-Gold II Myelin Staining Kit over Black Gold I are that it is more readily soluble, can be utilized at a higher concentration, produces a more uniform and consistent staining, takes less time to stain, and does not require post-staining intensification procedures.
The use of Black-Gold II is tailored to studies using formalin-fixed, non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated fibers appear black. This novel tracer can be used to localize both normal and pathological myelin. Black-Gold II can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, methamphetamine, acrylamide, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid.
The Black-Gold II Myelin Staining Kit also comes with sodium thiosulfate and cresyl violet. The sodium thiosulfate solution is required for extracting excess Black-Gold II stain. Whereas the cresyl violet is a Nissl stain that colors cell bodies a brilliant bluish hue and is used as a counter stain with Black-Gold II.
Black Gold II Purity: No detectable amount of uncomplexed gold was found.
Black Gold II Illumination: Bright field or dark field.
Black Gold II Solubility: Freely soluble in water, saline, or dilute acids.
Components
Black-Gold II: 150mg dry powder, yellow in color.
Cresyl Violet Stain: 150mg dry powder, purplish-black in color.
5% Sodium Thiosulfate: 10ml
Physical form
See Components
Storage and Stability
The kit can be stored at room temperature. Ideally, the Black-Gold II powder should be kept in a desiccator, because of its hygroscopic nature. The dry powder, if unopened and stored properly, is useable for up to one year from date of receipt. Once resuspended the Black-Gold II staining solution should be stored in the dark at 4°C and is good for up to 3 months.
TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100, Торговая марка BLACK-GOLD application(s) immunohistochemistry: suitable shipped in ambient Узнать цену -
CHKtide, biotin conjugate 12-414
Biochem/physiol ActionseCl@ss: 32160405 NACRES: NA.42
Protein Target: CHK
Target Sub-Family: CAMK
Quality
Routinely evaluated as a substrate for CHK enzymes.
Physical form
sterile, distilled water
Storage and Stability
1 year at -20°CLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Upstate® application(s) activity assay: suitable (kinase) NCBI accession no. NM_001274.3, UniProt accession no. O14757, shipped in dry ice Gene Information human ... CHEK1(1111) Узнать цену -
Chlorophenolred--D-galactopyranoside, Sodium Salt
Empirical Formula (Hill Notation): C25H21Cl2O10S · Na Molecular Weight: 607.39
Кат. номер 220588-20GM 220588-25MG 220588-5GM 220588-10GM 220588-250MG 220588-250MGCN General descriptionSubstrate for the spectrophotometric determination of -galactosidase activity. Exhibits ten-fold greater sensitivity for -galactosidase than o-NPG (Cat. No. 48712).
Substrate for spectrophotometric determination of -galactosidase activity. Exhibits about ten-fold greater sensitivity for -galactosidase than o-NPG (Cat. No. 48712,).
Packaging
250 mg in Plastic ampoule
Warning
Toxicity: Irritant (B)
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 week at 4°C.Other NotesEustice, D.C., et al. 1991. Biotechniques 11, 739.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C assay 98% (HPLC) Quality Level 100, form solid Торговая марка Calbiochem® storage condition OK to freeze, desiccated (hygroscopic) impurities <0.1% chlorophenolred color orange-red to red solubility water: 20 mg/mL shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Coelenterazine fcp
Empirical Formula (Hill Notation): C25H24FN3O2 CAS Number: 123437-33-2 Molecular Weight: 417.48 MDL number: MFCD18632537 PubChem Substance ID: 24892332 NACRES: NA.83
Biochem/physiol ActionsКат. номер C3105-50UG-PW C3105-50UG C3105-25UG
135 times higher luminescence intensity than native coelenterazineПараметрыQuality Level 200, assay ≥95% (HPLC) form solid solubility methanol: 1 mg/mL storage temp. −20°C SMILES string Oc1ccc(cc1)C2=CN3C(=O)C(Cc4ccc(F)cc4)=NC3=C(CC5CCCC5)N2 InChI 1S/C25H24FN3O2/c26-19-9-5-17(6-10-19)14-22-25(31)29-15-23(18-7-11-20(30)12-8-18)27-21(24(29)28-22)13-16-3-1-2-4-16/h5-12,15-16,27,30H,1-4,13-14H2 InChI key OLXXULUJSSTXSP-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Coelenterazine hcp
Empirical Formula (Hill Notation): C25H25N3O2 CAS Number: 123437-32-1 Molecular Weight: 399.48 MDL number: MFCD03453204 PubChem Substance ID: 24892609 NACRES: NA.83
Кат. номер C3355-25UG C3355-25UG-KC C3355-50UG-PW C3355-25UG-PW C3355-50UG General descriptionCoelenterazine hcp is a semisynthetic analog of coelenterazine.ApplicationCoelenterazine hcp has been used:- as a component of coelenterazine reagent in the reporter assay
- in the purification of Renilla luciferase-labeled Annexin V
- in evaluating the activity of aequorin in Escherichia coli
Biochem/physiol Actions
Coelenterazine hcp has 190 times higher luminescence intensity than native coelenterazine and a faster response time than native coelenterazine.ПараметрыQuality Level 200, assay ≥95% (HPLC) form solid solubility methanol: 1 mg/mL, clear, yellow to orange storage temp. −20°C SMILES string Oc1ccc(cc1)C2=CN3C(=O)C(Cc4ccccc4)=NC3=C(CC5CCCC5)N2 InChI 1S/C25H25N3O2/c29-20-12-10-19(11-13-20)23-16-28-24(21(26-23)14-17-8-4-5-9-17)27-22(25(28)30)15-18-6-2-1-3-7-18/h1-3,6-7,10-13,16-17,26,29H,4-5,8-9,14-15H2 InChI key YEGCUOQUFAXCMK-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAR-4M - Calbiochem
Empirical Formula (Hill Notation): C25H26N4O3 Molecular Weight: 430.50
Кат. номер 251760-1MG 251760-1MGCN General descriptionA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500,).
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
Packaging
1 mg in Plastic ampoule
Biochem/physiol Actions
Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Warning
Toxicity: Irritant (B)
Physical form
In DMSO.
Reconstitution
Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.Other NotesKojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.Legal InformationNot available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 98% (HPLC) form solution Торговая марка Calbiochem® storage condition OK to freeze, protect from light color red-violet fluorescence ex ~560 nm, em ~575 nm shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 2 Узнать цену -
DAR-4M AM - Calbiochem
Empirical Formula (Hill Notation): C28H31IN4O5 Molecular Weight: 630.47
Кат. номер 251765-1MG 251765-1MGCN General descriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760,) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.
Packaging
1 mg in Plastic ampoule
Packaged under inert gas
Biochem/physiol Actions
Product does not compete with ATP.
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Reversible: no
Warning
Toxicity: Irritant (B)
Physical form
In DMSO.
Reconstitution
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.Other NotesKojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.Legal InformationNot available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 98% (HPLC) form solution Торговая марка Calbiochem® storage condition OK to freeze, protect from light color dark red fluorescence ex ~560 nm, em ~575 nm shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 2 Узнать цену -
Dihydroethidium - CAS 38483-26-0 - Calbiochem
Empirical Formula (Hill Notation): C21H21N3 CAS Number: 38483-26-0 Molecular Weight: 315.41
Кат. номер 309800-25MG 309800-25MGCN General descriptionA cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
Packaging
25 mg in Glass bottle
Packaged under inert gas
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Useful fluorogenic probe for the detection of reactive oxygen species (ROS)
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Irritant (B)
Reconstitution
Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.Other NotesHaugen, T.S., et al. 1999. Eur. Respir. J.14, 1100.
Mancini, M., et al. 1997. J. Cell Biol.138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 95% (HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color pink solubility DMSO: 20 mg/mL, DMF: soluble shipped in ambient storage temp. 20°C InChI 1S/C21H21N3/c1-2-15-20(13-6-4-3-5-7-13)17-10-14(22)8-9-16(17)18-11-24-12-19(23)21(15)18/h3-12,15,20H,2,22-23H2,1H3 InChI key ZDWPSSBKIMIPMY-UHFFFAOYSA-N
Safety InformationWGK Germany WGK 3 Узнать цену -
Dihydrorhodamine 123 - CAS 109244-58-8 - Calbiochem
Empirical Formula (Hill Notation): C21H18N2O3 CAS Number: 109244-58-8 Molecular Weight: 346.38 MDL number: MFCD00285712
Кат. номер 309825-5MG 309825-5MGCN General descriptionCell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.
Cell-permeable fluorogenic probe that is useful for detection of reactive oxygen species (ROS), such as peroxide and peroxynitrite.
Packaging
5 mg in Plastic ampoule
Packaged under inert gas
Biochem/physiol Actions
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Detection of reactive oxygen species (ROS)
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.Other NotesCrow, J.P. 1997. Nitric Oxide1, 145.
Kim, Y.M., et al. 1996. Infect Immun. 64, 3074.
Williams, M.S., and Henkart, P.A. 1996. J. Immunol. 157, 2395.
van de Water, B., et al. 1995. Mol. Pharmacol. 48, 928.
Royall, J.A., and Ischiropoulos, H. 1993. Arch. Biochem. Biophys. 302, 348.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 95% (HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color pink-white solubility DMSO: 5 mg/mL shipped in ambient storage temp. 20°C InChI 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3 InChI key FNEZBBILNYNQGC-UHFFFAOYSA-N
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Dimethyl-Histone H3 (Lys27) Peptide, biotin conjugate 12-566
Storage and StabilityeCl@ss: 32160405 NACRES: NA.42
stable 2 years at -20°C from date of shipmentLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Upstate® application(s) immunoprecipitation (IP): suitable NCBI accession no. NM_002107.3, UniProt accession no. Q16695, shipped in dry ice Gene Information human ... H3F3B(3021) Узнать цену -
E-Toxa-Clean® Concentrate E9029-500G
NACRES: NA.26 ApplicationUse at 1% in hot tap water.
E-Toxa-Clean® Concentrate has been used to destroy all the endotoxins in the glasswares. It is also been used to destroy the contamination of low density lipoprotein (LDL)(+) and LDL() with lipopolysaccharide (LPS).
Biochem/physiol Actions
Alkaline detergent for pre-cleaning glassware prior to inactivation of endotoxin by steam sterilization or baking.Legal InformationE-Toxa-Clean is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H302 - H315 - H318 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 Personal Protective Equipment dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
E-Toxate™ Endotoxin standard
NACRES: NA.24
Кат. номер E8029-1VL-KC E8029-1VL ApplicationE-Toxate endotoxin standard was used along with the limulus amebocyte lysate assay kit to determine the concentration of endotoxins in isolated samples of human holotransferrin and apotransferrin used to treat primary hepatocyte cultures.Other NotesA preparation of E. coli 055:B5 lipopolysaccharide
Can be stored at 2-8 °C when stored separately from the E-Toxate kit.
Analysis Note
Standardized against USP reference standard endotoxin (RSE)Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, contains solubilizer storage temp. 2-8°C
Safety InformationPersonal Protective Equipment dust mask type N95 (US),, Eyeshields,, Gloves, RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
E-TOXATE™ Kit ET0200-1KT
NACRES: NA.84 ApplicationThe E-TOXATE Kit was used to determine the number of bacteria in suspensions of group B streptococci used to infect rats for models of sepsis.Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыusage sufficient for 20 assays Quality Level 200, storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
E-Toxate™ Kit ET0100-1KT
ApplicationE-TOXATE Kit was used to determine the number of bacteria in suspensions of group B streptococci used to infect rats for models of sepsis.Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, usage sufficient for 50 assays storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
E-TOXATE™ Kit ET0300-1KT
NACRES: NA.84 ApplicationE-TOXATE™ Kit has been used in the semiquantitative determination of endotoxin levels in 0.1 % (w/v) dispersions of polyelectrolyte nanoparticle construct (NPs) and 0.1 % (w/v) polymer solutions.Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыusage sufficient for 100 assays Quality Level 200, storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
E-Toxate™ Reagent
PackagingКат. номер 2108-10VL-C 2108-10VL 2108-1VL-C 2108-10VL-PW 2108-100VL 2108-1VL
10 vials in glass insert
Components
10 mL of Catalog No. 2107 ("Endotoxin-free" water) is supplied with each 1-vial and 10-vial order. 2 x 10 mL is supplied with 100-vial orders.Other NotesReady-to-use single test vials containing E-TOXATE reagent. Pipette test solution directly into the vial.Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100, sensitivity measuring range 0.05-0.1 EU/mL storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
E-Toxate™ reagent from Limulus polyphemus
NACRES: NA.26
Кат. номер E8779-5X2ML E8779-2ML-PW E8779-2ML ApplicationThe endotoxins from Salmonella typhimurium isolated from human waste samples were detectable at a low concentration of 0.5 µg/ml using E-TOXATE reagent.Legal InformationE-Toxate is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100, form dry concentrate usage sufficient for 20 tests detection sensitivity measuring range 0.05-0.1 EU/mL storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Factor Xa chromogenic substrate
MDL number: MFCD00678994 NACRES: NA.83
Кат. номер F3301-25MG-PW F3301-25MG ApplicationFactor Xa chromogenic substrate has been used in factor Xa protease assay for placenta organelle protein and Prospirobolus joannsi.
Biochem/physiol Actions
Factor Xa chromogenic substrate contains peptide sequence Bz-Ile-Glu-Gly-Arg-pNA. Factor Xa mediates the release of p-NA chromogen post hydrolysis of substrate which is measurable photometrically.
Physical properties
KM for human factor Xa is ~0.8mMПараметрыassay 90% (HPLC) Quality Level 200, form solid mol wt apparent mol wt 622.7 solubility methanol: water (1:9): 25 mg/mL storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fast Green FCF
Empirical Formula (Hill Notation): C37H34N2Na2O10S3 Linear Formula: C37H34N2O10S3Na2 CAS Number: 2353-45-9 Molecular Weight: 808.85 Colour Index Number: 42053 Beilstein/REAXYS Number: 5718212 EC Number: 219-091-5 MDL number: MFCD00013053 PubChem Substance ID: 57654190 NACRES: NA.83
Кат. номер F7252-100G F7252-5G F7252-25G F7252-10G General descriptionFast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.ApplicationFast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.Packaging5, 25, 100 g in glass bottleBiochem/physiol ActionsFast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.Параметрыform powder Quality Level 200 composition Dye content, 85% mp 290 °C (dec.) (lit.) solubility H2O: 1 mg/mL, clear SMILES string [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O InChI 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2 InChI key RZSYLLSAWYUBPE-UHFFFAOYSA-L
Safety Informationpictograms GHS08 signalword Warning hcodes H341 pcodes P201 - P308 + P313 Personal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Filipin complex from Streptomyces filipinensis
CAS Number: 11078-21-0 MDL number: MFCD00131073 NACRES: NA.77
Кат. номер F9765-25MG-PW F9765-50MG F9765-25MG F9765-50MG-PW General descriptionChemical structure: macrolideApplicationFilipin complex from Streptomyces filipinensis has been used as a profluorescence probe to detect the free cholesterol in cell.
Packaging
25 mg in poly bottle
50 mg in glass bottle
Biochem/physiol Actions
Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action is unclear but may be due to altering membrane permeability. Filipin binds to model crystalline phase lipid bilayers containing cholesterol, and to liquid phase bilayers with or without cholesterol, in an orientation that forms membrane pores.It both inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.Other NotesFilipin contains 8 isomeric components, with filipin III as the major component.Параметрыbiological source Streptomyces sp. (Streptomyces filipinensis) Quality Level 200, assay 70% (UV) form powder solubility methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow Mode of action cell membrane | interferes antibiotic activity spectrum fungi storage temp. 20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Filipin complex ready made solution
Кат. номер SAE0088-1ML SAE0088-1ML-PW General descriptionFilipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.ApplicationFilipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.
Biochem/physiol Actions
Filipin is an antibiotic with antifungal functionality. Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol. On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.Other NotesFilipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).Параметрыassay ≥90% (HPLC) form liquid storage condition protect from light shipped in dry ice storage temp. −20°C
Safety InformationWGK Germany WGK 2 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Filipin III from Streptomyces filipinensis
Empirical Formula (Hill Notation): C35H58O11 CAS Number: 480-49-9 Molecular Weight: 654.83 MDL number: MFCD00466923 PubChem Substance ID: 24894871 NACRES: NA.77
Кат. номер F4767-5MG-PW F4767-5MG F4767-1MG F4767-1MG-PW ApplicationFilipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.Packaging1, 5 mg in glass bottleBiochem/physiol ActionsFilipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.Other NotesFilipin III is the major component of 8 isomeric components in filipin.ПараметрыQuality Level 300 assay 85% (HPLC) form powder mp 163-180 °C solubility methanol: 9.80-10.20 mg/mL, clear, colorless to yellow Mode of action cell membrane | interferes antibiotic activity spectrum fungi, yeast storage temp. 20°C SMILES string CCCCCC(O)C1C(O)CC(O)CC(O)CC(O)CC(O)CC(O)CC(O)C(C)=C\C=C\C=C\C=C\C=C\C(O)C(C)OC1=O InChI 1S/C35H58O11/c1-4-5-11-16-31(42)34-33(44)22-29(40)20-27(38)18-25(36)17-26(37)19-28(39)21-32(43)23(2)14-12-9-7-6-8-10-13-15-30(41)24(3)46-35(34)45/h6-10,12-15,24-34,36-44H,4-5,11,16-22H2,1-3H3/b7-6+,10-8+,12-9+,15-13+,23-14- InChI key IMQSIXYSKPIGPD-BWJOQPJXSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 - H335 pcodes P302 + P352 - P305 + P351 + P338 Target Organs Respiratory system Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Filipin III ready made solution
Кат. номер SAE0087-1ML SAE0087-1ML-PW General descriptionFilipin III is a polyene macrolide antibiotic and the major component isolated from cultures of S. filipinensis. 1 Due to its ability to fluoresce and bind to cholesterol, Filipin has been widely
used as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for free cholesterol in the study of Type C Niemann-Pick disease. 3 Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. 4 Filipin III was found to trigger signaling responses in tobacco cells, including a NADPH oxidase-dependent production. 5. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane.ApplicationFilipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.Other Notes- Filipin III solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C avoid freeze thaw cycles.
- Filipin III is used in various concentrations depending on the specific protocol used. Dilute 1mg/ml stock solution with appropriate buffer according to the used protocol.
- Filipin III interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
ПараметрыQuality Level 200, assay ≥95% (HPLC) form liquid storage condition protect from light shipped in dry ice storage temp. −20°C
Safety InformationWGK Germany WGK 1 Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Firefly Lantern Extract FLE250-1VL
NACRES: NA.81 General descriptionThe bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.ApplicationFireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
Packaging
1 vial in serum bottle
Biochem/physiol Actions
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.Other NotesCrude luciferin and luciferase
Reconstitution
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.ПараметрыQuality Level 200, form solution packaging pkg of 250 mg dried lanterns application(s) toxicology assay: suitable storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
FSB - CAS 891180-93-1 - Calbiochem
Empirical Formula (Hill Notation): C24H17FO6 CAS Number: 760988-03-2 Molecular Weight: 420.39
Кат. номер 344101-5MG 344101-5MGCN General descriptionA fluorine analog of the amyloidophilic fluorescent probe BSB (Cat. No. 286895) that crosses the blood-brain barrier and displays low toxicity (LD50 >80 mg/kg). Suitable for non-invasive amyloid visualization in living transgenic Tg2576 mice by using 19F and 1H-MRI (Magnetic Resonance Imaging). Also useful for staining plaques and neurofibrillary tangles in brain tissue sections of Alzheimers diseased patients with improved sensitivity, and reduced background.
A fluorine analog of the amyloidophilic fluorescent probe BSB (Cat. No. 286895,) that crosses the blood-brain barrier and displays low toxicity (LD50 >80 mg/kg). Suitiable for non-invasive amyloid visualization in living transgenic Tg2576 mice by using 19F and 1H-MRI (Magnetic Resonance Imaging). Also useful for staining plaques and neurofibrillary tangles in brain tissue sections of Alzheimers diseased patients with improved sensitivity, and reduced background.
Packaging
5 mg in Plastic ampoule
Packaged under inert gas
Biochem/physiol Actions
Primary Target
Amyloidophilic fluorescent probe
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.Other NotesHiguchi, M., et al. 2005. Nat. Neurosci.8, 527.
Sato, K., et al. 2004. Eur. J. Med. Chem.39, 573.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 95% (HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color yellow solubility DMSO: 200 mg/mL shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Heparanase-1 pre-activated human SAE0116-10UG
Enzyme Commission (EC) Number: 3.2.1.166 ( BRENDA | IUBMB ) General descriptionRecombinant human heparanase-1 is expressed in human HEK 293 cells as an active heterodimer glycoprotein containing a 50 kDa subunit and a 8 kDa subunit. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Biochem/physiol Actions
Heparanase-1 (heparanase) is the only heparan sulfate degrading endoglycosidase. It is involved in the regulation of multiple biological processes that increase tumor growth, metastasis, angiogenesis and inflammation. Heparanase is involved in ECM remodeling and heparan sulfate-linked biological molecules release, including cytokines and growth factors. Heparanase cleaves the heparan sulfate side chains of heparan sulfate proteoglycans into fragments of 10–20 sugar units.
Heparanase is initially translated as a preproenzyme containing a signal peptide. Cleavage of the signal sequence yields a latent 65 kDa pro-heparanase, which must undergo further processing for activity. Mature active heparanase is a heterodimer consisting an N-terminal 8 kDa subunit and a C-terminal 50 kDa subunit.
Unit Definition
one heparanase activity unit is the amount of heperanase required to cleave 1 pmol of pentasaccharide to disaccharide and trisaccharide in one minute, in 37°C, pH 5.0Параметрыrecombinant expressed in HEK 293 cells assay 95.0% (SDS-PAGE) form lyophilized powder impurities 1.00 EU/µg (LAL) UniProt accession no. Q9Y251, shipped in ambient storage temp. 20°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Histone H3 Peptide, biotin conjugate 12-403
eCl@ss: 32160405 NACRES: NA.41 ApplicationThis peptide corresponds to residues 1-21 of histone H3.
Quality
Routinely evaluated as a substrate for histone-modifying enzymes.
Physical form
Lyophilized powder
Storage and Stability
2 years at -20°CLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Upstate® application(s) immunoprecipitation (IP): suitable NCBI accession no. NM_002107.3, UniProt accession no. Q16695, shipped in wet ice Gene Information human ... H3F3B(3021) Узнать цену -
Histone H3 Peptide, biotin conjugate, residues 21-44 12-404
eCl@ss: 32160405 NACRES: NA.41 ApplicationThis gene contains introns & its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Quality
Routinely evaluated by as a substrate for histone-modifying enzymes.
Physical form
Lyophilized
Storage and Stability
Lyophilized: 2 years at 4°C. Rehydrated: 6 months at -20°C.Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Upstate® application(s) immunoprecipitation (IP): suitable NCBI accession no. NM_002107.3, UniProt accession no. Q16695, shipped in wet ice Gene Information human ... H3F3B(3021) Узнать цену -
Histone H4 Peptide, biotin conjugate, residues 2-24 12-372
QualityeCl@ss: 32160405 NACRES: NA.41
Routinely evaluated by HAT Assay as a substrate in histone acetyl transferase (HAT) and deacetylase assays. For additional information, please refer to HAT and HDAC assay kits (catalog #17-289 and #17-320).Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 100 Торговая марка Upstate® application(s) affinity precipitation assay: suitable","immunoprecipitation (IP): suitable NCBI accession no. NM_003538.3, UniProt accession no. P62805, shipped in dry ice Узнать цену -
Indoxyl phosphate disodium salt
Empirical Formula (Hill Notation): C8H6NNa2O4P CAS Number: 3318-43-2 Molecular Weight: 257.09 EC Number: 222-014-8 MDL number: MFCD00040646 PubChem Substance ID: 24896070 NACRES: NA.83
SubstratesКат. номер I5505-1G I5505-250MG I5505-100MG I5505-50MG
Histochemical substrate for alkaline phosphatase.ПараметрыQuality Level 200, assay ≥94% (HPLC) form crystalline solubility water: 5 mg/mL, clear to slightly hazy storage temp. −20°C SMILES string [Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccccc12 InChI 1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2 InChI key WCSKWBKPKXJWEG-UHFFFAOYSA-L
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JC-1 - CAS 47729-63-5 - Calbiochem
Empirical Formula (Hill Notation): C25H27Cl4IN4 CAS Number: 47729-63-5 Molecular Weight: 652.23
Кат. номер 420200-5MG 420200-5MGCN General descriptionA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential m in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
Packaging
5 mg in Plastic ampoule
Packaged under inert gas
Biochem/physiol Actions
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Used as a ratiometric indicator of mitochondrial potential m in cells, tissues, and isolated mitochondria
Warning
Toxicity: Irritant (B)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.Other NotesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100, assay 95% (HPLC) form solid Торговая марка Calbiochem® storage condition OK to freeze, protect from light color red-orange solubility DMSO: 5 mg/mL shipped in ambient storage temp. 20°C
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Luciferase from Photinus pyralis (firefly)
CAS Number: 61970-00-1 Enzyme Commission (EC) Number: 1.13.12.7 ( BRENDA | IUBMB ) MDL number: MFCD00131546 NACRES: NA.54
Кат. номер SRE0045-10MG-PW SRE0045-1MG-PW SRE0045-2MG SRE0045-10MG SRE0045-1MG SRE0045-2MG-PW ApplicationFirefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function.
Biochem/physiol Actions
Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light.
Unit Definition
One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 µl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 µCi of 14C in a PPO/POPOP cocktail.
Physical form
Supplied as a lyophilized powder containing HEPES, pH 7.5, NaCl, MgCl2, EDTA, DTT and a carbohydrate stabilizer.
This product is a recombinant luciferase (62 kDa) from Photinus pyralis (American firefly) produced from the luc gene expressed in E. coli.
Preparation Note
To obtain maximal solubility it is important to reconstitute the enzyme at a high salt concentration, such as 1 M Tris buffer with any counter ion at pH 7-8. The enzyme can be prepared at a concentration of up to 5 mg protein/ml. Do not vortex and avoid agitation.
After reconstitution, the enzyme solutions can kept at 4-8 °C for up to 2 days or frozen in working aliquots at -20°C for at least one month. Repeated freezing and thawing is not recommended.ПараметрыQuality Level 200, recombinant expressed in E. coli form lyophilized powder specific activity 10x1010 units/mg protein mol wt 62 kDa Featured Industry Diagnostic Assay Manufacturing storage temp. 20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Luciferase from Vibrio fischeri (Photobacterium f)
CAS Number: 9014-00-0 Enzyme Commission (EC) Number: 1.14.14.3 ( BRENDA | IUBMB ) EC Number: 232-751-7 MDL number: MFCD00131545 NACRES: NA.54
Кат. номер L8507-100MG-PW L8507-500MG-PW L8507-100MG L8507-500MG L8507-10MG L8507-25MG ApplicationLuciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
Packaging
100, 500 mg in poly bottle
Features and Benefits
Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde.Other NotesATCC No. 7744 balance primarily buffer salts and stabilizer.
Physical form
Partially purified lyophilized powderПараметрыform lyophilized powder Quality Level 200, composition Protein, ~40% biuret storage temp. 20°C
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Luminol
Empirical Formula (Hill Notation): C8H7N3O2 CAS Number: 521-31-3 Molecular Weight: 177.16 Beilstein/REAXYS Number: 383929 EC Number: 208-309-4 MDL number: MFCD00006890 PubChem Substance ID: 24891343 NACRES: NA.83
Кат. номер A8511-5G A8511-25G A8511-1G General descriptionLuminol is a chemiluminescent compound, which belongs to the class of cyclic acyl hydrazides. It exhibits chemiluminescence, when oxidized by oxidizing agents. Luminol may be associated with nausea, vomiting and mucosa irritation in eyes and skin.ApplicationLuminol has been used in chemiluminescence assay.
Packaging
5, 25 g in glass bottle
Features and Benefits
Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.ПараметрыQuality Level 200, assay ≥97% (HPLC) form powder or chunks mp >300 °C (lit.) solubility DMSO: 50 mg/mL, clear to slightly hazy SMILES string Nc1cccc2C(=O)NNC(=O)c12 InChI 1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13) InChI key HWYHZTIRURJOHG-UHFFFAOYSA-N
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MTT - CAS 298-93-1 - Calbiochem
Empirical Formula (Hill Notation): C18H16BrN5S CAS Number: 298-93-1 Molecular Weight: 414.32 MDL number: MFCD00011964
Кат. номер 475989-10GM 475989-1GM 475989-1GMCN 475989-10GMCN US2475989-1GM General descriptionMembrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.
Packaging
1 g in Glass bottle
10 g in Plastic ampoule
Biochem/physiol Actions
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.
Warning
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.Other NotesLiu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +15C to +30C Quality Level 100, assay 98% (silver nitrate titration) form solid Торговая марка Calbiochem® storage condition OK to freeze impurities 0.5% Sulfated ash color yellow solubility PBS: 2 mg/mL cation traces heavy metals: 0.001% shipped in ambient storage temp. 15-25°C InChI 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1 InChI key AZKSAVLVSZKNRD-UHFFFAOYSA-M
Safety Informationpictograms GHS07,GHS08 signalword Warning hcodes H315 - H319 - H335 - H341 pcodes P201 - P202 - P261 - P302 + P352 - P305 + P351 + P338 - P308 + P313 Target Organs Respiratory system WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
N-(4-Aminobutyl)-N-ethylisoluminol
Empirical Formula (Hill Notation): C14H20N4O2 CAS Number: 66612-29-1 Molecular Weight: 276.33 Beilstein/REAXYS Number: 920895 MDL number: MFCD00010559 PubChem Substance ID: 24890404 NACRES: NA.83
PackagingКат. номер A0156-25MG A0156-250MG A0156-10MG A0156-5MG A0156-100MG
25, 250 mg in glass bottle
Features and Benefits
Efficient chemiluminescent NH2-coupling reagent for detection of a wide variety of proteins down to the picomole range.Other NotesReportedly, the use of this material in chemiluminescent assays has distinct advantages over conventional radioimmunoassay.ПараметрыQuality Level 100,, 200, assay 90% form powder mp 259-260 °C (lit.) solubility glacial acetic acid: 50 mg/mL, clear, colorless to yellow storage temp. 2-8°C SMILES string CCN(CCCCN)c1ccc2C(=O)NNC(=O)c2c1 InChI 1S/C14H20N4O2/c1-2-18(8-4-3-7-15)10-5-6-11-12(9-10)14(20)17-16-13(11)19/h5-6,9H,2-4,7-8,15H2,1H3,(H,16,19)(H,17,20) InChI key LEOJISUPFSWNMA-UHFFFAOYSA-N
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Naphthol AS-BI -D-glucuronide
Empirical Formula (Hill Notation): C24H22BrNO9 CAS Number: 37-87-6 Molecular Weight: 548.34 Beilstein/REAXYS Number: 6883214 MDL number: MFCD00067162 PubChem Substance ID: 24897531 NACRES: NA.83
PackagingКат. номер N1875-100MG N1875-250MG N1875-25MG N1875-500MG N1875-5MG
25, 100, 250, 500 mg in poly bottle
Substrates
Substrate for histochemical demonstration of β-glucuronidase.ПараметрыQuality Level 200, assay 99% (TLC) form powder solubility 1 M NH4OH: 50 mg/mL, clear, colorless to faintly yellow storage temp. 20°C SMILES string COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2O[C@@H]4O[C@@H]([C@@H](O)[C@H](O)[C@H]4O)C(O)=O InChI 1S/C24H22BrNO9/c1-33-16-5-3-2-4-15(16)26-22(30)14-9-12-8-13(25)7-6-11(12)10-17(14)34-24-20(29)18(27)19(28)21(35-24)23(31)32/h2-10,18-21,24,27-29H,1H3,(H,26,30)(H,31,32)/t18-,19-,20+,21-,24+/m0/s1 InChI key ACOOAEDFQKSZTC-NABGWTBKSA-N
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Naphthol AS-BI N-acetyl--D-glucosaminide
Empirical Formula (Hill Notation): C26H27BrN2O8 CAS Number: 3395-37-7 Molecular Weight: 575.41 MDL number: MFCD00076131 PubChem Substance ID: 24897360 NACRES: NA.83
Кат. номер N4006-1G N4006-100MG N4006-500MG N4006-50MG ПараметрыQuality Level 200, assay 97% (TLC) form powder solubility DMF: 50 mg/mL storage temp. 20°C SMILES string COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OC4OC(CO)C(O)C(O)C4NC(C)=O InChI 1S/C26H27BrN2O8/c1-13(31)28-22-24(33)23(32)21(12-30)37-26(22)36-20-11-14-7-8-16(27)9-15(14)10-17(20)25(34)29-18-5-3-4-6-19(18)35-2/h3-11,21-24,26,30,32-33H,12H2,1-2H3,(H,28,31)(H,29,34) InChI key DMLKUPXKUQREOC-UHFFFAOYSA-N
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Naphthol AS-BI phosphate disodium salt hydrate
Empirical Formula (Hill Notation): C18H13BrNNa2O6P · xH2O CAS Number: 207569-04-8 Molecular Weight: 496.16 (anhydrous basis) PubChem Substance ID: 24897558 NACRES: NA.83
Кат. номер N2250-1G N2250-5G N2250-50MG N2250-500MG N2250-100MG General descriptionNaphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.ApplicationNaphthol AS-BI phosphate disodium salt hydrate is suitable to use:- in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
- as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
- to perform alkaline phosphatase reaction
Biochem/physiol Actions
Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.
Substrates
Substrate for the histochemical demonstration of acid and alkaline phosphatase.ПараметрыQuality Level 200, assay ≥98.5% (HPLC) form powder solubility water: 50 mg/mL, clear, colorless to yellow storage temp. −20°C SMILES string O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O InChI 1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2 InChI key WNPKMQCBZKBNOS-UHFFFAOYSA-L
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Naphthol AS-D chloroacetate
Empirical Formula (Hill Notation): C20H16ClNO3 CAS Number: 35245-26-2 Molecular Weight: 353.80 EC Number: 252-463-5 MDL number: MFCD00042714 PubChem Substance ID: 24897441 NACRES: NA.83
Кат. номер N0758-1G N0758-100MG N0758-250MG N0758-500MG N0758-5G ApplicationNaphthol AS-D chloroacetate has been in a substrate solution for naphthol-AS-D-chloroacetate-esterase (CIAE) staining.
Packaging
100, 250, 500 mg in poly bottle
1 g in poly bottle
Substrates
Substrate for histochemical localization of esterasesПараметрыQuality Level 200, assay 98% (TLC) form powder solubility acetone: 50 mg/mL, clear, colorless to faintly yellow storage temp. 20°C SMILES string Cc1ccccc1NC(=O)c2cc3ccccc3cc2OC(=O)CCl InChI 1S/C20H16ClNO3/c1-13-6-2-5-9-17(13)22-20(24)16-10-14-7-3-4-8-15(14)11-18(16)25-19(23)12-21/h2-11H,12H2,1H3,(H,22,24) InChI key FMVKYSCWHDVMGO-UHFFFAOYSA-N
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