Protein Electrophoresis & Western Blotting
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Immobilon Crescendo Western HRP substrate, 20 mL WBLUR0020
eCl@ss: 42029053 NACRES: NB.22 General descriptionPremixed, ready to use chemiluminescent HRP detection reagentApplicationFor use on immunoblots.
Western blotting, dot/slot blotting
Packaging
1 bottle for 200 cm2
Quality
The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.
Storage and Stability
Stable for 1 year at 4°C from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.Legal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 2 to 8 °C packaging pkg of 100 mL Торговая марка Immobilon® application(s) dot blot: suitable, western blot: suitable membrane area 200 cm2 , membrane coverage conjugate peroxidase conjugate detection method chemiluminescent shipped in ambient storage temp. 2-8°C Узнать цену -
Immobilon ECL Ultra Western HRP Substrate
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBULS0100 WBULS0100-20ML General descriptionImmobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.ApplicationImmobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.
Sufficient for 1000 cmComponentsSolution A- 50 mL
Solution B- 50 mLStorage and StabilityStore at 2–8 °CLegal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300 application(s) western blot: suitable detection method chemiluminescent shipped in ambient Узнать цену -
Immobilon ECL Ultra Western HRP Substrate WBULS0500
eCl@ss: 42029053 General descriptionImmobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.ApplicationWestern blotting, HRP
"Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.
Sufficient for 5000 cm"ComponentsSolution A- 250 mL
Solution B- 250 mLStorage and StabilityStore at 2–8 °CLegal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 300 application(s) western blot: suitable detection method chemiluminescent shipped in ambient Узнать цену -
Immobilon Forte Western HRP substrate, 100 mL
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBLUF0500 WBLUF0100 General descriptionPremixed, ready to use chemiluminescent HRP detection reagentApplicationFor use on immunoblots.
Western blotting, dot/slot blottingPackaging1 bottle for 1000 cm2QualityThe reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.Storage and StabilityStable for 1 year at room temperature from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.Legal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 18 to 24 °C packaging pkg of 100 mL Торговая марка Immobilon® application(s) western blot: suitable membrane area 1000 cm2 , membrane coverage conjugate peroxidase conjugate detection method chemiluminescent shipped in ambient
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immobilon Forte Western HRP substrate, 20 mL WBLUF0020
eCl@ss: 42029053 NACRES: NB.22 General descriptionPremixed, ready to use chemiluminescent HRP detection reagentApplicationFor use on immunoblots.
Western blotting, dot/slot blotting
Packaging
1 bottle for 200 cm2
Quality
The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.
Storage and Stability
Stable for 1 year at room temperature from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.Legal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 18 to 24 °C packaging pkg of 20 mL Торговая марка Immobilon® application(s) dot blot: suitable, western blot: suitable membrane area 200 cm2 , membrane coverage conjugate peroxidase conjugate detection method chemiluminescent shipped in ambient storage temp. 15-25°C
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immobilon Signal Enhancer for Immunodetection
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBSH0500 WBSH0500-100ML General descriptionImmobilon® Signal Enhancer combines signal amplification and blocking in one ready-to-use reagent.
Signal Enhancing: Amplify low signal intensity Western blots without amplifying the noise
Save Primary Antibody: When the signal is strong, use Immobilon Signal Enhancer to reduce the amount of valuable primary antibody requiredApplicationImmobilon® Signal Enhancer is designed for use in Western blotting applications when low signal intensity is observed. It combines signal amplification and blocking in one ready-to-use reagent. Enhancement of the antibody-antigen reaction can produce a 2 to 10-fold increase in signal intensity over traditionally used reagents. The reagent is formulated for use in both membrane blocking and antibody dilution steps and can be used for traditional immunodetection or with the SNAP i.d. Protein Detection System. Immobilon Signal Enhancer is compatible with PVDF as well as nitrocellulose blotting membranes and can be used with chromogenic, chemiluminescent, and fluorescent detection methods.Legal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 300 Торговая марка Immobilon® application(s) western blot: suitable detection method chemiluminescent shipped in wet ice
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Warning hcodes H290 - H315 - H317 - H319 - H411 pcodes P234 - P261 - P273 - P280 - P302 + P352 - P305 + P351 + P338 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immobilon Western Chemiluminescent HRP Substrate
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBKLS0050 WBKLS0500 WBKLS0100 ApplicationFor use on immunoblots.Legal InformationImmobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 2 to 8 °C Quality Level 400 packaging pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution Торговая марка Immobilon® application(s) western blot: suitable membrane area 5000 cm2 , membrane coverage conjugate peroxidase conjugate detection method chemiluminescent shipped in wet ice Узнать цену -
Immobilon® Block - CH (Chemiluminescent Blocker)
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBAVDCH01-100ML WBAVDCH01 General descriptionImmobilon® Block Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Blk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques.ApplicationFor use on immunoblots.
Packaging
Sufficient reagent for 19 mini blots (8x7 cm)
Quality
For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.
Storage and Stability
Stable for 2 years at 20–25°CLegal InformationBlk is a trademark of Merck KGaA, Darmstadt, Germany
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 20 to 25 °C Quality Level 300, packaging pkg of 500 mL Торговая марка Blk™ application(s) western blot: suitable membrane area 1000 cm2 , membrane coverage conjugate peroxidase conjugate detection method chemiluminescent shipped in ambient storage temp. -140 to -196°C
Safety Informationpictograms GHS07 signalword Warning hcodes H317 - H412 pcodes P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immobilon® Block - FL (Fluorescent Blocker)
eCl@ss: 42029053 NACRES: NB.22
Кат. номер WBAVDFL01-100ML WBAVDFL01 General descriptionImmobilon® Block Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Blk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques.ApplicationFor use on immunoblots.
Packaging
Sufficient reagent for 19 mini blots (8x7 cm)
Quality
For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.
Storage and Stability
Stable for 2 years at 20–25°CLegal InformationBlk is a trademark of Merck KGaA, Darmstadt, Germany
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 20 to 25 °C Quality Level 300, packaging pkg of 500 mL Торговая марка Blk™ application(s) western blot: suitable membrane area 1000 cm2 , membrane coverage detection method fluorometric shipped in ambient storage temp. -140 to -196°C
Safety InformationFlash Point F does not flash Flash Point C does not flash Узнать цену -
Immobilon® Block - PO (Phosphoprotein Blocker)
eCl@ss: 42029053 NACRES: NB.22
QualityКат. номер WBAVDP001-100ML WBAVDP001
For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.
Storage and Stability
Stable for 1 year at 20–25°CLegal InformationBlk is a trademark of Merck KGaA, Darmstadt, Germany
Immobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions 20 to 25 °C Quality Level 300, packaging pkg of 500 mL Торговая марка Blk™ application(s) western blot: suitable membrane area 1000 cm2 , membrane coverage detection method chemiluminescent (phosphoprotein), fluorometric (phosphoprotein) shipped in ambient storage temp. -140 to -196°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immunoblot Blocking Reagent 20-200
eCl@ss: 42029053 NACRES: NB.22 ApplicationFor use in western blots as a blocking agent.QualityRoutinely evaluated by western blotPhysical formnon-fat dry milk fortified with vitamin A palmitate and vitamin D2, containing no preservativesStorage and Stability2 years at 4°CLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыТорговая марка Upstate® application(s) western blot: suitable shipped in wet ice Узнать цену -
Phosphate buffered saline
MDL number: MFCD00131855 NACRES: NA.25
Кат. номер P4739-10PAK P4739-10VL ApplicationPhosphate buffered saline has been used as a blocking buffer in dot blot or Western blot applications.ПараметрыQuality Level 100 form powder (dry milled) composition nonfat dry milk , potassium chloride, 76.5% , potassium phosphate monobasic anhydrous, 0.3% , sodium chloride, 12.2% , sodium phosphate dibasic, 10.7% pH 7.3
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Protein A, HRP conjugate 18-160
eCl@ss: 32160405 NACRES: NB.22 ApplicationSuitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.Physical formchromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease freeStorage and StabilityLyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°C.Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыТорговая марка Upstate® application(s) ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, western blot: suitable shipped in wet ice 344,14 € / 33 246 ₽Получить предложение -
Protein G, HRP conjugate 18-161
eCl@ss: 32160405 NACRES: NB.22 ApplicationSuitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.QualitySuitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistryPhysical formchromatographically pure Protein G conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/ml BSA.Storage and StabilityLyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°CLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыТорговая марка Upstate® application(s) ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, western blot: suitable shipped in wet ice 332,50 € / 32 122 ₽Получить предложение -
SeaBlock Reagent, Salmon Plasma
Кат. номер 558300-500ML 558300-500MLCN General descriptionA ready-to-use blocking reagent for Western blotting and immunoassays. Contains salmon plasma proteins that do not interact with mammalian antibodies, thus offering a reduced background.
A ready-to-use blocking reagent containing non-mammalian proteins that do not interact with mammalian antibodies.
Packaging
500 ml in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 0.09% NaN₃, pH 7.4.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C form liquid Торговая марка Calbiochem® storage condition OK to freeze shipped in ambient storage temp. 2-8°C
Safety InformationWGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SIGMAFAST™ 3,3-Diaminobenzidine tablets
CAS Number: 91-95-2 MDL number: MFCD00007725 PubChem Substance ID: 24893872 NACRES: NA.83
Кат. номер D4418-5SET D4418-50SET ApplicationDeveloped for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packetsReconstitutionEach tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.Legal InformationSIGMAFAST is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form tablet solubility water: 1 tablet/5 mL storage temp. 20°C SMILES string Nc1ccc(cc1N)-c2ccc(N)c(N)c2 InChI 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2 InChI key HSTOKWSFWGCZMH-UHFFFAOYSA-N
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H302 - H315 - H319 - H335 - H341 - H350 pcodes P202 - P261 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313 Target Organs Respiratory system RIDADR UN 3316 9 WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SignalBoostImmunoreaction Enhancer Kit
Кат. номер 407207-1KIT 407207S-1KIT 407207-1KITCN General descriptionSignalBoost Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.
• Increases signal: minimizes background resulting in high signal to noise ratios
• Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
• Convenient: ready-to-use format, no dilution necessary
SignalBoost enhances antigen-antibody reactions, yielding signals several times to several tens of times higher than those obtained with conventional methods. It has been verified that enhancement is particularly marked for relatively inactive antibodies. Compositions optimized for primary and secondary antibodies respectively minimize background and yield high S/N ratios. It can be used for various assays involving antigen-antibody reactions, such as immunoblotting, dot blotting and ELISA. Components do not affect the activity of enzymes used for labeling (peroxidase, alkaline phosphatase, etc.), allowing use in assays using any of these enzymes. It can also be used for detection of both fluorescence and coloration. For convenience, SignalBoost is supplied in a ready-to-use form, is at an appropriate concentration for use, and does not need to be diluted. Simply dilute antibodies with this product instead of conventional diluents to use. It does not lengthen the time required for the assay.
Packaging
1 kit in Plastic ampoule
Components
Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol.
Warning
Toxicity: Standard Handling (A)
Principle
SignalBoost Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays.
Physical form
Format: Liquid, Antibody Diluent
Storage and Stability
Upon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C usage sufficient for 1600 cm2, sufficient for 20 miniblot(s) Торговая марка Calbiochem® storage condition OK to freeze, protect from light detection method chemiluminescent, colorimetric, fluorometric shipped in wet ice storage temp. 2-8°C 367,41 € / 35 494 ₽Получить предложение -
Streptavidin SA101
eCl@ss: 32160405 NACRES: NB.22 Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Chemicon® application(s) ELISA: suitable, western blot: suitable conjugate unconjugated shipped in wet ice
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Streptavidin, HRP conjugate 18-152
eCl@ss: 32160405 NACRES: NB.22 ApplicationSuitable for use as a detection reagentPhysical form0.05M HEPES, 0.1M Na2SO4, pH6.8, preservative.Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 100 Торговая марка Upstate® application(s) ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable shipped in wet ice Узнать цену -
Streptavidin, Peroxidase Conjugated SA202
eCl@ss: 32160405 ApplicationWORKING DILUTION
The final working dilution is dependent on the specific assay conditions. However, good results can be expected with dilutions in the following ranges:
ELISA: 1/500 - 1/10,000
Immunohistochemistry: 1/40 - 1/1,000
1 mL Streptavidin, Peroxidase Conjugated for ELISA, Western Blotting & IHC.Physical formThe conjugate is supplied in phosphate buffered saline, pH 7.2, with 0.5% (w/v) bovine serum albumin and 10% (w/w) glycerol as stabilizers, plus added preservative.Storage and StabilityStore at +2 to +8°C, for up to 6 months.
NOTE: Do not dilute into buffers containing sodium azide, as this inhibits peroxidase.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыТорговая марка Chemicon® application(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable conjugate peroxidase conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TMB Enhanced One Component HRP Membrane Substrate
NACRES: NA.83
Кат. номер T9455-100ML-PW T9455-1L T9455-100ML T9455-1L-PW General descriptionTMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.CautionStable at least one year at 2-8 °C.Physical formReady-to-use.ПараметрыQuality Level 200 form solution storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Western Blocker™ Solution
NACRES: NA.25
Кат. номер W0138-400ML W0138-400ML-KC W0138-PH W0138-400ML-PW W0138-500ML General descriptionWestern Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.ApplicationWestern Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.Features and Benefits- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal InformationWestern Blocker is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 shelf life 2 yr storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Узнать цену -
Western-Re-Probe Reagent
Кат. номер WB59-100ML WB59-100MLCN General descriptionWestern-Re-Probe Reagent is specifically formulated to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity. The removal of antibodies also removes corresponding chemiluminescence or radioactive signals from the blots. Stripped blots can be probed with new probes.
This Western-Re-Probe Reagent is validated for use in Immunoblotting.
Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
Packaging
100 ml in Plastic ampouleApplicationImmunoblotting (see comments)
Components
5X Re-Probe solution (125 ml)
Warning
Toxicity: Standard Handling (A)
Physical form
Supplied as a 5X solution.Other NotesSufficient volume to strip 25-30 standard blots.
ADVANTAGES
-Immunoblotting analyzed repeatedly with multiple probes
-Saving on hard-to-get reagents and samples
-Correction and re-analysis of unsatisfactory Western Blots
-Saving time spent in gel re-running and transfer processes
PROTOCOL
1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe in water.
2- Incubate the membrane in 1X Western-Re-Probe buffer for 30 min at room temperature (RT) with shaking.
3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
4- Block the membrane and perform immunodetection.
Western-Re-Probe Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
PROTOCOL SUMMARY
1. Incubate membrane in 1X Western-Re-Probe (30 min at RT) with shaking
2. Wash the membrane 3 times in PBST or TBST buffer
3. Block membrane and perform immunodetectionLegal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid Торговая марка Calbiochem® storage condition OK to freeze shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Immobilon® GO for Simple Immunodetection IMGDV002
eCl@ss: 42029053 General descriptionImmobilon® GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot.ApplicationFor use on immunoblots, Western blots and dot blots.Legal InformationHIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
Immobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature description Immobilon® GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot. Торговая марка Immobilon® application(s) dot blot: suitable, western blot: suitable compatibility for use with Immobilon Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with TBS-T with NFDM, for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
Immobilon® GO for Simple Immunodetection IMGDV010
eCl@ss: 42029053 ApplicationFor use on immunoblots, Western blots and dot blots.
Immobilon® GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot.Legal InformationHIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
Immobilon is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature description Immobilon® GO applies the principles of lateral flow assays for walk away processing of Western blots. Simply place your miniblot in the device and then add your wash and antibody solutions to the ports in the top. Return later to apply detection substrate and image your Western blot. Торговая марка Immobilon® application(s) dot blot: suitable, western blot: suitable compatibility for use with Immobilon Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with TBS-T with NFDM, for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Double MultiBlot (4.5 x 8.4 cm) SNAP2MB3
eCl@ss: 42029053 NACRES: NB.22 General descriptionThis system includes the SNAP i.d. 2.0 Base, two MultiBlot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideApplicationFor use on immunoblots.
Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane.LinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Midi (8.5 x 13.5 cm) SNAP2MIDI
eCl@ss: 42029053 NACRES: NB.22 General descriptionThis system includes the SNAP i.d. 2.0 Base, two Midi Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideApplicationFor use on immunoblots.
Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane..LinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Midi and MultiBlot (8.5 x 13.5 cm and 4.5 x 8.4 cm) SNAP2MB2
eCl@ss: 42029053 NACRES: NB.22 General descriptionThis system includes the SNAP i.d. 2.0 Base, a Midi Blot Holding Frame,a MultiBlot Holding Frame, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideApplicationFor use on immunoblots.
Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane.LinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Mini (7.5 x 8.4 cm) SNAP2MINI
eCl@ss: 42029053 NACRES: NB.22 General descriptionThis system includes the SNAP i.d. 2.0 Base, two Mini Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideLinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Mini and Midi (7.5 x 8.4 cm and 8.5 x 13.5 cm)
eCl@ss: 42029053
Кат. номер SNAP2MM-B SNAP2MM General descriptionThis system includes the SNAP i.d. 2.0 Base, a Mini Blot Holding Frame,a Midi Blot Holding Frame, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideApplicationFor use on immunoblots.LinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric storage temp. room temp Узнать цену -
SNAP i.d. 2.0 Protein Detection System-Mini and MultiBlot (7.5 x 8.4 cm and 4.5 x 8.4 cm) SNAP2MB1
eCl@ss: 42029053 NACRES: NB.22 General descriptionThis system includes the SNAP i.d. 2.0 Base, a Mini Blot Holding Frame,a MultiBlot Holding Frame, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start GuideApplicationFor use on immunoblots.
Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology & a built-in flow distributor actively drive reagents through the membrane.LinkageReplaces: WBAVDBASELegal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Antibody Collection Tray SNAPABTR
eCl@ss: 42029053 NACRES: NB.22 General descriptionRecover greater than 80% of your Antibody with the Antibody Collection Tray specially designed for the SNAP i.d. systemsApplicationFor use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Blot Roller SNAP2RL
eCl@ss: 42029053 NACRES: NB.22 ApplicationNew Blot Roller specially designed for use with the SNAP i.d. 2.0 system.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Midi Blot Holders (8.5 x 13.5 cm) SNAP2BHMD0100
eCl@ss: 42029053 NACRES: NB.22 General descriptionThe new Midi Blot Holders ensure the membrane is horizontal allowing uniform Antibody distribution. The flat profile alows for compact space allocation for storage, shipping and waste management.ApplicationThe new Midi Blot Holders ensure the membrane is horizontal allowing uniform Antibody distribution. The flat profile alows for compact space allocation for storage, shipping & waste management.
For use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Midi Blot Holding Frame (single pack)
eCl@ss: 42029053 NACRES: NB.22
Кат. номер SNAP2FRMD02 SNAP2FRMD01 General descriptionThe new Midi Blot Holding Frames are stackable and have clear lids to retain humidity. They have an easy locking latch and an umbrella valve for offline incubation.ApplicationFor use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient Узнать цену -
SNAP id® 2.0 Mini Blot Holders (7.5 x 8.4 cm) SNAP2BHMN0100
eCl@ss: 42029053 NACRES: NB.22 General descriptionThe new Mini Blot Holders ensure the membrane is horizontal allowing uniform Antibody distribution. The flat profile alows for compact space allocation for storage, shipping and waste management.ApplicationFor use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Mini Blot Holding Frame (single pack) SNAP2FRMN01
eCl@ss: 42029053 NACRES: NB.22 Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 Mini Blot Holding Frames (double pack) SNAP2FRMN02
eCl@ss: 42029053 NACRES: NB.22 General descriptionThe new Mini Blot Holding Frames are stackable and have clear lids to retain humidity. They have an easy locking latch and an umbrella valve for offline incubation.ApplicationThe new Mini Blot Holders ensure the membrane is horizontal allowing uniform Antibody distribution. The flat profile alows for compact space allocation for storage, shipping & waste management.
For use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 MultiBlot Holders (4.5 x 8.4 cm) SNAP2BHMB050
eCl@ss: 42029053 ApplicationFor use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
SNAP id® 2.0 MultiBlot Holding Frame (single pack) SNAP2FRMB01
eCl@ss: 42029053 NACRES: NB.22 General descriptionThe new MultiBlot Holding Frames are stackable and have clear lids to retain humidity. They have an easy locking latch and an umbrella valve for offline incubation.ApplicationThe new MultiBlot Holders ensure that each half blot membrane is aligned horizontally, allowing for the uniform distribution of antibody. The flat profile alows for compact space allocation for storage, shipping and waste management.
For use on immunoblots.Legal InformationSNAP id is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions Room Temperature Quality Level 200 Торговая марка SNAP id® application(s) western blot: suitable compatibility for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents detection method chemiluminescent, colorimetric, fluorometric shipped in ambient storage temp. room temp Узнать цену -
4X SDS Sample Buffer
Кат. номер 70607-3 70607-3CN General descriptionThe 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at 2X for most applications.Packaging2 ml in Plastic ampouleWarningToxicity: Harmful (C)Legal InformationNOVAGEN is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Торговая марка Novagen® storage condition OK to freeze, avoid repeated freeze/thaw cycles shipped in ambient, dry ice storage temp. 20°C
Safety InformationWGK Germany WGK 2 Узнать цену -
Adapters for mPAGE® gels when using XCell SureLock® and other compatible electrophoresis tanks MPTA
General descriptionAdapters for mPAGE gels when using XCell SureLockand other compatible electrophoresis tanks.- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationUse these adapters with XCell SureLock and other compatible electrophoresis tanks. Not required when using Bio-Rad Mini-PROTEAN systems.- Western Blotting
Legal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLC
XCell SureLock is a registered trademark of Life TechnologiesПараметрыpackaging pack of 2 ea Узнать цену -
MES SDS running buffer powder for mPAGE® Bis-Tris gels MPMES
General descriptionMES SDS running buffer powder for mPAGEBis-Tris gels. 5 packets. Each paclet makes 1 liter of running buffer.- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationMES SDS buffer is formulated for running mPAGE Bis-Tris gels. This buffer is recommended for separating small- and medium- sized proteins.
Reconstitute 1 pouch of buffer powder with 1000 ml deionized water to make 1X running buffer. Do not use acid or base to adjust the pH.- Western Blotting
Legal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pack of 5 ea (Each packet makes 1 liter of running buffer)
Safety Informationpictograms GHS02 signalword Warning hcodes H228 pcodes P210 - P240 - P241 - P280 - P370 + P378 WGK Germany WGK 2 Узнать цену -
MOPS SDS running buffer powder for mPAGE® Bis-Tris gels MPM0PS
General descriptionMOPS SDS running buffer powder for mPAGE Bis-Tris gels. 5 packets. Each packet makes 1 liter of running buffer.- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationMOPS SDS buffer is formulated for running mPAGE Bis-Tris gels. This buffer is recommended for separating large and medium-sized proteins.
Reconstitute 1 pouch of buffer powder with 1000 ml deionized water to make 1X running buffer. Do not use acid or base to adjust the pH.- Western Blotting
Legal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pack of 5 ea (Each packet makes 1 liter of running buffer)
Safety Informationpictograms GHS02 signalword Warning hcodes H228 pcodes P210 - P240 - P241 - P280 - P370 + P378 WGK Germany WGK 2 Узнать цену -
mPAGE® 10% Bis-Tris Precast Gel MP10W10
General descriptionmPAGE 10% Bis-Tris Precast Gel, 10x8 cm- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationmPAGE 10% bis-tris gels for high resolution protein separation- Load up to 80 µl sample / well
- Typical run time 26 min (MES, 180V) or 31 min (MOPS, 200V)
- Reverse gasket when using with Bio-Rad Mini-PROTEAN systems
- Use adapter (MPTA) with XCell SureLock and other compatible systems
Western BlottingLegal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 gels L x W x wells 10 cm x 8 cm x 10 well
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
mPAGE® 10% Bis-Tris Precast Gel MP10W15
General descriptionmPAGE 10% Bis-Tris Precast Gel, 10x8 cm- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationmPAGE 10% bis-tris gels for high resolution protein separation- Load up to 80 µl sample / well
- Typical run time 26 min (MES, 180V) or 31 min (MOPS, 200V)
- Reverse gasket when using with Bio-Rad Mini-PROTEAN systems
- Use adapter (MPTA) with XCell SureLock and other compatible systems
Western BlottingLegal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 gels L x W x wells 10 cm x 8 cm x 15 well
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
mPAGE® 10% Bis-Tris Precast Gel MP10W12
General descriptionmPAGE 10% Bis-Tris Precast Gel, 10x8 cm- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationmPAGE 10% bis-tris gels for high resolution protein separation- Load up to 80 µl sample / well
- Typical run time 26 min (MES, 180V) or 31 min (MOPS, 200V)
- Reverse gasket when using with Bio-Rad Mini-PROTEAN systems
- Use adapter (MPTA) with XCell SureLock and other compatible systems
Western BlottingLegal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 gels L x W x wells 10 cm x 8 cm x 12 well
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
mPAGE® 12% Bis-Tris Precast Gel MP12W10
General descriptionmPAGE 12% Bis-Tris Precast Gel, 10x8 cm- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ApplicationmPAGE 12% bis-tris gels for high resolution protein separation- Load up to 80 µl sample / well
- Typical run time 30 min (MES, 180V) or 34 min (MOPS, 200V)
- Reverse gasket when using with Bio-Rad Mini-PROTEAN systems
- Use adapter (MPTA) with XCell SureLock and other compatible systems
Western BlottingLegal InformationMPAGE is a registered trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 gels L x W x wells 10 cm x 8 cm x 10 well
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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