Carboxypeptidase G from Pseudomonas sp.

Carboxypeptidase G from Pseudomonas sp., or -Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NOcGMP signaling in lung adenocarcinoma cells.
Packaging
5 units in poly bottle
20 units in glass bottle
Biochem/physiol Actions
Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves -glutamyl pteroyl poly--glutamate yielding pteroyl--glutamate (folic acid) and free glutamate. It is considered highly specific for the -glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
Unit Definition
One unit will hydrolyze 1.0 µmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
Physical form
Contains sodium acetate salt.
Preparation Note
Chromatographically purified
Solutions should be prepared fresh prior to use.