HIV Protease, His tagged,recombinant from HIV-1 SRP2152-10UG

NACRES:

NA.26

HIV Protease has been used in in vitro detection of HIV protease activity using FRET-HIV Sensor, a Forster resonance energy transfer-based HIV protease-sensitive sensor.
Biochem/physiol Actions
The HIV-1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV-1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV-1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids is contributed by one of the monomers. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. The order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV-1) infection and AIDS have been shown to suppress the replication of HIV-1 and extend the life expectancy of HIV-1-infected individuals.
Sequence
PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
Physical form
Formulated in 20mM Mes buffer, pH6, 500mM KCl, 20% glycerol.
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Please keep in −20 °C for long term storage. It lost 70% activity in 4°C for one week. Freeze thaw cycle resistance.

biological source	human immunodeficiency virus 1
recombinant	expressed in E. coli
assay	85% (SDS-PAGE)
form	frozen liquid
mol wt	~11.9 kDa
packaging	pkg of 10 µg
storage condition	avoid repeated freeze/thaw cycles
concentration	200 µg/mL
color	colorless to clear
NCBI accession no.	AF459156,
UniProt accession no.	Q8JB49,
shipped in	dry ice
storage temp.	70°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable