Furin human SRP6274-10UG

NACRES:

NA.32

Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg′). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.
Physical form
Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

biological source	human
recombinant	expressed in HEK 293 cells
assay	95% (SDS-PAGE)
form	lyophilized powder
mol wt	calculated mol wt 66.5 kDa","observed mol wt 55-66 kDa (DTT-reduced. Asp 108 is the predicted N-terminus .)
packaging	pkg of 10 µg
impurities	<1 EU/µg endotoxin (LAL test)
conjugate	6-His tagged (C-terminus)
UniProt accession no.	P09958,
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... PACE(5045)

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable