Ficin from fig tree latex

CAS Number:	9001-33-6
Enzyme Commission (EC) Number:	3.4.22.3 ( BRENDA | IUBMB )
EC Number:	232-599-1
MDL number:	MFCD00081597
NACRES:	NA.54

Кат. номер

F4165-PM

F4165-1KU

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
Packaging
1000 units in poly bottle
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab)2 fragments from mouse IgG1.
Unit Definition
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.
One unit will produce a A280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

form	powder
Quality Level	200,
specific activity	≥0.1 unit/mg solid
mol wt	23.8 kDa
storage temp.	−20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1