Elastase from porcine pancreas

CAS Number:	39445-21-1
Enzyme Commission (EC) Number:	3.4.21.36 ( BRENDA | IUBMB )
EC Number:	254-453-6
MDL number:	MFCD00130998
NACRES:	NA.54

Кат. номер

E1250-CP

E1250-100MG

E1250-50MG

E1250-25MG

E1250-500MG

E1250-10MG

Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase.
The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development. It has also been used during the isolation of type II pneumocytes from human lungs.
Elastase from porcine pancreas has been used:

• to induce abdominal aortic aneurysm (AAA)
• to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice
• to digest aortas for aortic smooth muscle cells (SMC) isolation
• as a positive control of proteolytic digestion

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Package size based on protein content
One unit will hydrolyze 1.0 µmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
2x crystallized

Quality Level	200
type	Type I
form	suspension
specific activity	≥4.0 units/mg protein
mol wt	25.9 kDa
contains	0.1% thymol
concentration	0.5-15.0 mg/mL in water
foreign activity	trypsin ≤50 BAEE units/mg protein
storage temp.	2-8°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable