Carboxypeptidase B from porcine pancreas

CAS Number:	9025-24-5
Enzyme Commission (EC) Number:	3.4.17.2 ( BRENDA | IUBMB )
EC Number:	232-788-9
MDL number:	MFCD00081476
PubChem Substance ID:	24893175

Кат. номер

C9584-5MG

C9584-1MG

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.
Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
Packaging
1, 5 mg in glass bottle
Package size based on protein content
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2-dipyridyl.
Unit Definition
One unit will hydrolyze 1.0 µmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.
Physical form
Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note
Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.
Analysis Note
Protein determined by biuret.

form	lyophilized powder
Quality Level	200,
specific activity	≥125 units/mg protein
mol wt	34,000  Da± 600
purified by	affinity chromatography
composition	Protein, 40-70%
foreign activity	carboxypeptidase A ≤1%, chymotrypsin ≤0.1%, trypsin ≤5%
storage temp.	−20°C
InChI	1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key	TWURVFFNODFJBJ-UHFFFAOYSA-N

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable