Human Dermal Fibroblasts: HDF, adult 106-05A

NACRES:

NA.81

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Skin
injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response
Fibroblast Basal Medium containing 10% FBS & 10%

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10%
• DMSOCan be cultured at least 16 doublings

Fibroblast Growth Medium (116-500), all-in-one ready to use media
Please refer to the HDF Culture Protocol,.

Quality Level	100,
biological source	human neonatal foreskin or adult skin (normal)
packaging	pkg of 500,000 cells
growth mode	Adherent
karyotype	2n = 46
morphology	Fibroblast
application(s)	cell culture | mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

RIDADR

NONH for all modes of transport