GenElute™ Plasmid Miniprep Kit PLN350-1KT

NACRES:

NA.52

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 µg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

• Purified plasmid DNA in less than 30 minutes
• Purify up to 15 µg of plasmid DNA
• No detectable genomic DNA or RNA contamination
• No phenol/chloroform extraction or alcohol precipitation required

GenElute is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200
usage	sufficient for 350 purifications
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry.
storage temp.	15-25°C

pictograms	GHS02,GHS05,GHS07,GHS08
signalword	Danger
hcodes	H226 - H290 - H302 - H315 - H319 - H334 - H336
pcodes	P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
Target Organs	Central nervous system
RIDADR	UN 3316 9