GenElute™ HP Plasmid Miniprep Kit NA0160-1KT

NACRES:

NA.52

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.
The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 µg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.

• Purified plasmid DNA in less than 30 minutes
• Up to 25 µg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• No phenol/chloroform extraction or alcohol precipitation required

GenElute is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200
usage	sufficient for 350 preparations
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry.
storage temp.	15-25°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H290 - H302 - H314 - H334 - H336
pcodes	P234 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable