Chloramphenicol

Empirical Formula (Hill Notation):	C11H12Cl2N2O5
Linear Formula:	Cl2CHCONHCH(CH2OH)CH(OH)C6H4NO2
CAS Number:	56-75-7
Molecular Weight:	323.13
Beilstein/REAXYS Number:	2225532
EC Number:	200-287-4
MDL number:	MFCD00078159
PubChem Substance ID:	24892448
NACRES:	NA.76

Кат. номер

C1919-5G

C1919-25G

C1919-100G

Chemical structure: phenicole
5, 25, 100 g in glass bottle
Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 µg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.
Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.
Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.
Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.
Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.
Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.
Keep container tightly closed in a dry and well-ventilated place, Light sensitive. Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Quality Level	200,
product line	BioReagent
form	powder
application(s)	cell culture | plant: suitable
impurities	Endotoxin, tested
mp	148-150 °C (lit.)
solubility	ethanol: 50 mg/mL
Featured Industry	Agriculture
Mode of action	protein synthesis | interferes
antibiotic activity spectrum	Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma
SMILES string	OC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O
InChI	1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
InChI key	WIIZWVCIJKGZOK-RKDXNWHRSA-N
Gene Information	human ... CYP1A2(1544)

pictograms	GHS05,GHS08
signalword	Danger
hcodes	H318 - H351 - H361fd
pcodes	P202 - P280 - P305 + P351 + P338 - P308 + P313 - P405 - P501
Personal Protective Equipment	Eyeshields,, Gloves,, type P1 (EN143) respirator filter,, type P3 (EN 143) respirator cartridges,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3