SYBR® Green JumpStart™ Taq ReadyMix™

MDL number:	MFCD03458666
NACRES:	NA.55

Кат. номер

S4438-100RXN

S4438-500RXN

S4438-20RXN

SYBR® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling.
Features and Benefits

• This master mix allows consistency from one reaction to the next
• Designed to minimize contamination
• Reduced primer dimers
• Reduced set-up time as compared to manual or wax HotStart® methods
• Allows for room temperature set-up and contains a fluorescent dye; Ideal for qPCR applications

• Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield.
• SYBR Green JumpStart Taq ReadyMix for SYBR based QPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments.

Packaging
Default reaction volume is 50 µL
20RXN is packaged as 1 X 500 µL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL
SYBR Green JumpStart Taq ReadyMix combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2x concentrate for ease of use. Simply add 25 µL of the 2x mix to DNA template, primers and water. The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.
Principle
SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigmas SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Quality Level	200,
form	liquid
feature	hotstart
concentration	1.25 units/reaction (50 µL reaction volume)
application(s)	qPCR: suitable
Featured Industry	Agriculture
compatibility	for use with ABI 5700
detection method	SYBR® Green
shipped in	wet ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable