REDAccuTaq® LA DNA Polymerase

MDL number:	MFCD01092095
NACRES:	NA.55

Кат. номер

D4812-250UN

D4812-250UN-TAQ

D4812-2.5KU-TAQ

D4812-50UN

D4812-2.5KU

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
Produce long DNA amplicons for:

• Mutation analysis
• Cloning genes
• DNA sequencing
• cDNA library generation

Features and Benefits

• Increased fidelity, up to 6.5x that of Taq DNA polymerase
• Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
• Visual confirmation that enzyme has been added, and proper mixing has occurred
• No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

Packaging
The enzyme is provided with an optimized 10x reaction buffer for enhanced amplification of complex templates.
REDAccuTaq® LA DNA polymerase is a blend of Sigmas high quality Taq DNA polymerase, a small amount of an additional polymerase that exhibits 35 exonuclease or proofreading activity, and our inert red dye. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchasers own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchasers activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDAccuTaq is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	liquid
feature	Long & Accurate PCR, dNTPs included: no, hotstart: no
concentration	1 unit/µL
application(s)	PCR: suitable
color	red
shipped in	wet ice
storage temp.	20°C

Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable