KiCqStart® Probe qPCR ReadyMix™, ROX™

NACRES:

NA.55

Кат. номер

KCQS06-SAMPLE

KCQS06-5000RXN

KCQS06-250RXN

KCQS06-1250RXN

KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:

• Applied Biosystems® 5700
• Applied Biosystems 7000
• Applied Biosystems 7300
• Applied Biosystems 7700
• Applied Biosystems 7900
• Applied Biosystems 7900 HT Fast
• Applied Biosystems 7900 HT
• Applied Biosystems StepOnePlus™
• Applied Biosystems StepOne™

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of Qiagen Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

form	liquid
storage condition	protect from light
application(s)	qPCR: suitable
color	light blue
detection method	probe-based
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable