JumpStart™ REDTaq® ReadyMix™ Reaction Mix

MDL number:

MFCD00166026

Кат. номер

P0982-100RXN

P0982-20RXN

P0982-800RXN

P0982-20RXN-KC

P0982-PH

P0476-40RXN-PW

P0982-100RXN-PW

P0982-20RXN-PW

P0982-10ML

JumpStart REDTaq ReadyMix PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigmas ReadyMix and REDTaq. The mix includes Sigmas JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2x optimized reaction concentrate. Add 25 µL of the ReadyMix to primers, template, and water to a final reaction volume of 50 µL.
JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored.
The red dye allows quick visual confirmation the enzyme has been added and properly mixed. After PCR, an aliquot may be loaded directly onto an agarose gel without the addition of loading buffers. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired.
Using JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.
Jumpstart™ REDTaq® ReadyMix™ Reaction Mix is used for direct DNA extraction and amplification using PCR. It has also been used for:

• qualitative multiplex PCR
• methylation-specific PCR (MSPCR)
• PCR amplification of cDNA synthesized from total RNA

• JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
• REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
• Inert red dye allows for easy verification that reagent has been mixed properly
• Other loading dyes not necessary. Aliquots may be directly loaded onto an agarose gel after the reaction

Default reaction volume is 50 µL
20RXN is packaged as 1 X 500 µL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.
For a typical PCR reaction, mix 25 mL of JumpStart™ REDTaq® ReadyMix™ with 25 mL of a mixture containing template DNA, primers, and water. Reaction volume can be scaled if desired.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchasers own internal research. No other patent rights (such as 5 Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200
form	liquid
feature	dNTPs included, hotstart
concentration	2.5 units/reaction (50 µL reaction volume)
application(s)	PCR: suitable
color	red
Featured Industry	Agriculture
shipped in	wet ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P280
RIDADR	NONH for all modes of transport
WGK Germany	3