JumpStart™ REDTaq® DNA Polymerase

MDL number:	MFCD00166026
NACRES:	NA.55

Кат. номер

D8187-2.5KU

D8187-250UN

D8187-50UN

D8187-2.5KU-TAQ

D8187-250UN-TAQ

JumpStart REDTaq DNA Polymerase is Sigmas high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.

• The hot start Taq antibody prevents non-specific product formation.
• Assembled PCR reactions can be placed at room temperature up to 2 hours.
• Red tracer allows visible tracking of enzyme addition and reaction mixing.
• PCR can be directly loaded onto an agarose gel without addition of loading buffers.
• The red tracer is a tracking dye that run at 125bp on a 1% agarose gel.

Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
JumpStart™ REDTaq® DNA Polymerase has been used in:

• polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)
• endothelial cells DNA derived from reverse transcribed RNA
• leg genomic DNA from cricket flies

Features and Benefits

• Prevents amplification of nonspecific products, resulting in increased efficiency and higher yields of the desired sequence
• No lengthy activation step is required for enzyme activity to be restored
• Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
• Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye additions

Packaging
The enzyme is provided with an optimized 10x reaction buffer.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	liquid
feature	dNTPs included: no, hotstart
concentration	1 unit/µL
application(s)	PCR: suitable
color	red
shipped in	wet ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319 - H335
pcodes	P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable