JumpStart™ AccuTaq™ LA DNA Polymerase

NACRES:

NA.55

Кат. номер

D5809-500UN

D5809-125UN

D5809-500UN-TAQ

D5809-1500UN-TAQ

D5809-125UN-TAQ

D5809-1500UN

JumpStart™ AccuTaq™ LA DNA Polymerase has been used in polymerase chain reaction(PCR) for the amplification of soil DNA samples and in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs).
JumpStart AccuTaq LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5x of Taq DNA polymerase).
Features and Benefits

• JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Up to 6.5x greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
• Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Packaging
Supplied with 10x reaction buffer.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchasers own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchasers activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
Jacobs is a registered trademark of Jacobs
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	liquid
feature	Long & Accurate PCR, dNTPs included: no, hotstart
concentration	2.5 unit/µL
application(s)	PCR: suitable
color	colorless
shipped in	wet ice
storage temp.	20°C

Personal Protective Equipment	Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable