Enhanced Avian Reverse Transcriptase [eAMV™ RT]

MDL number:	MFCD01634820
NACRES:	NA.55

Кат. номер

A4464-1KU

A4464-500UN

Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Biochem/physiol Actions
Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.
Features and Benefits

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb

Packaging
Provided with a vial of 10x reaction buffer.
Unit Definition
One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	crystalline powder
concentration	20 units/µL
color	colorless
shipped in	wet ice
storage temp.	20°C

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable