- Производитель:
- Roche
Enzyme Commission (EC) Number: | 2.7.7.7 ( BRENDA | IUBMB ) |
Кат. номер |
4728858001 |
4728866001 |
4728904001 |
4728882001 |
4728874001 |
Taq DNA Polymerase is a highly processive 53 DNA polymerase that lacks 35 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Contents
Taq DNA Polymerase, 5 U/µl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.
- PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
Features and Benefits
- Reliable reproducible results: High lot-to-lot consistency.
- No need to test each lot: Taq DNA Polymerase is rigorously tested.
- Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.
Packaging
1 kit containing 3 components
Quality
Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/µl
Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
Quality Level | 100, |
description | Number of Reactions: If 1.25 U are used per 50 µL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage. |
usage | sufficient for 2,000 reactions (04728904001), sufficient for 4,000 reactions (04728858001), sufficient for 400 reactions (04728874001), sufficient for 80 reactions (04728866001), sufficient for 800 reactions (04728882001) |
feature | dNTPs included, hotstart: no |
packaging | pkg of 1,000 U (04728882001 [4 x 250 U]), pkg of 2,500 U (04728904001 [10 x 250 U]), pkg of 5,000 U (04728858001 [20 x 250 U]), pkg of 100 U (04728866001), pkg of 500 U (04728874001 [2 x 250 U]) |
Торговая марка | Roche |
parameter | 72 °C optimum reaction temp. |
application(s) | PCR: suitable |
optimum pH | ~9.0 (20 °C) |
shipped in | dry ice |
storage temp. | 20°C |
RIDADR | NONH for all modes of transport |
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