Expand™ High FidelityPLUS PCR System

Enzyme Commission (EC) Number:

2.7.7.7 ( BRENDA | IUBMB )

Кат. номер

3300234001

3300226001

The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.
The Expand™ High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:

• PCR
• RT-PCR
• Primer extension
• Carryover prevention

Features and Benefits

• Safeguard against cross-contamination.

This is the choice for large numbers of amplifications.

• Obtain sixfold higher fidelity and higher yields,

compared to Taq DNA Polymerase.

• Efficiently label DNA.

Use this kit to label DNA with radioactive or nonradioactive nucleotides.
Packaging
1 kit containing 4 components
Quality
Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.
Unit Definition
Volume Activity: 5 U/μL
For life science research only. Not for use in diagnostic procedures.
Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Expand is a trademark of Roche

usage	sufficient for 2,500 reactions (03300234001), sufficient for 500 reactions (03300226001)
Quality Level	100,
feature	High Fidelity PCR, dNTPs included: no, hotstart: no
packaging	pkg of 2,500 U (03300234001 [10x250 U]), pkg of 500 U (03300226001 [2x250 U])
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	20°C

RIDADR

NONH for all modes of transport