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Guanidine hydrochloride

Гарантия 1 год

Производитель:: Sigma-Aldrich

Онлайн консультант Анна Головацкая Получить консультацию эксперта

Производитель:: Sigma-Aldrich

Empirical Formula (Hill Notation):	CH₅N₃ · HCl
Linear Formula:	NH₂C(=NH)NH₂ · HCl
CAS Number:	50-01-1
Molecular Weight:	95.53
Beilstein/REAXYS Number:	3591990
EC Number:	200-002-3
MDL number:	MFCD00013026
PubChem Substance ID:	24895119
NACRES:	NA.31

Кат. номер

G3272-100G

G3272-1KG

G3272-EW

G3272-2KG

G3272-500G

G3272-25G

Application

Guanidine hydrochloride has been used:

as a component of the extraction buffer for the extraction of proteoglycans
in extraction during protein fractionation of ATDC5 cell lines
as a chemical additive to study its effective absorbance spectra in structural analysis
to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Packaging

1, 2 kg in poly bottle
100, 500 g in poly bottle

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Параметры

biological source	synthetic (organic)
Quality Level	400
grade	for molecular biology
assay	99%
form	crystalline powder
application(s)	RNA extraction: suitable
impurities	0.3% water (Karl Fischer)
color	white
pH	(25 °C, 4.6 - 6/573 g/L)
mp	180-185 °C (lit.)
solubility	H₂O: 6 M, clear, colorless
density	1.3 g/cm³ (lit.)
cation traces	Pb: 5 ppm
UV absorption	: 260 nm A_max: 0.10, : 290 nm A_max: 0.05
foreign activity	DNase, RNase, none detected
SMILES string	Cl[H].NC(N)=N
InChI	1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
InChI key	PJJJBBJSCAKJQF-UHFFFAOYSA-N
Gene Information	human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

Safety Information

Pictograms	GHS07
Signal Word	Warning
Hazard Statements	H302 + H332 - H315 - H319
Precautionary Statements	P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Пуско-наладка

Выполним распаковку, визуальную проверку, сборку и установку, запуск и настройку, проверку функциональности, проведем инструктаж персонала.

Техническое обслуживание

Проведем периодические регламентные работы: визуальный осмотр, очистка засоренных узлов и частей, замена расходных материалов и изношенных деталей, тестирование прибора, проверка показателей и измерение параметров, калибровка и настройка, обновление программного обеспечения.

IQ/OQ/PQ квалификация, валидация

Проведем монтажную (IQ), операционную (OQ) и эксплуатационную (PQ) квалификацию оборудования по протоколам производителя в полном соответствии с стандартами GMP/GLP.

Ремонт

Проведем диагностику в нашем сервисном центре или у Вас на предприятии, выявим причину поломки, устраним неисправность, проведем тестирование, настройку, калибровку отремонтированного оборудования.