DIG Oligonucleotide Tailing Kit, 2nd generation 3353583910

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides.
DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in:

• northern blot assay
• in situ hybridization (ISH)
• fluorescence in situ hybridization (FISH)

In addition to common hybridization techniques, DIG-labeled oligonucleotides are especially useful for screening expression libraries for sequence-specific DNA binding proteins, for example, transcription factors.
Features and Benefits
Tailing of oligonucleotides at the 3-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).

• Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
• Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells

Packaging
1 kit containing 11 components
Principle
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).
Preparation Note
Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 µg of a 30-mer oligonucleotide) can be applied.
Storage and Stability
Store at -15–-25 °C. (unopened kit)
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice

pictograms	GHS07,GHS08,GHS09
signalword	Danger
hcodes	H302 + H332 - H350i - H360F - H411
pcodes	P201 - P261 - P273 - P280 - P308 + P313 - P391
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash