DIG DNA Labeling and Detection Kit 11093657910

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3-OH termini of random oligonucleotides as primers.
Contents:

• Unlabeled Control DNA 1, 100 µg/ml
• Unlabeled Control DNA 2, 200 µg/ml
• DNA Dilution Buffer
• DIG-labeled Control DNA, 5.2 µg/ml
• 10x Hexanucleotide Mix
• 10x dNTP Labeling Mixture
• Klenow Enzyme, Labeling grade, 2 U/µl
• Anti-digoxigenin-AP-conjugate, 750 U/ml
• NBT/BCIP Concentrated Stock Solution
• Blocking Reagent

Specificity
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:

• Southern blots
• Northern blots
• Dot blots
• Colony and plaque screening

DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.
Packaging
1 kit containing 10 components.
Preparation Note
Working concentration: Dilution of Antibody 1:5,000
For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 25 labeling reactions, sufficient for 50 blots
Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable