Deoxyribonuclease I from bovine pancreas

CAS Number:	9003-98-9
Enzyme Commission (EC) Number:	3.1.21.1 ( BRENDA | IUBMB )
EC Number:	232-667-0
MDL number:	MFCD00130918

Кат. номер

DN25-100MG

DN25-10MG

DN25-PM

DN25-5G

DN25-1G

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.

• Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
• Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
• DNase I structure resembles the structure of to exonuclease III. It includes two central sheets. Each sheet is composed of six -strands. This complex of sheets is surrounded by extensive loop and -helical regions. This enzyme shares structural similarity to exonuclease III.

[1]

• Decreases viscosity providing better yields by removing DNA in primary cell isolation:
• Incorporating labelled bases into DNA: DNA nick
• Radioactive labelling
• Bioprocessing applications: DNA removal
• Eliminating genomic DNA from RNA preparations before RT-PCR
• In vitro transcription
• Nick translation
• DNase footprinting
• Actin regulation of actin polymerization in cells, and cell apoptosis
• UV crosslinking of proteins to nucleic acids
• DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process

[1]
Used for the removal of DNA from protein samples.
10, 100 mg in poly bottle
1, 5 g in poly bottle
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Our Deoxyribonuclease DNAse I,is essentially RNAse free product, which support the product application.
One Kunitz unit will produce a change in A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate.
Crude preparation, contains calcium chloride
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
Protein determined by biuret.

Quality Level	300
form	lyophilized powder
specific activity	400 Kunitz units/mg protein
mol wt	~31 kDa
composition	Protein, 85%
solubility	0.15 M NaCl: soluble 5.0 mg/mL, hazy
Featured Industry	Diagnostic Assay Manufacturing Diagnostic Assay Manufacturing
foreign activity	RNase 0.02%
shipped in	wet ice
storage temp.	20°C

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 + P312 - P501
Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable