SYBR® Green I nucleic acid gel stain

CAS Number:	163795-75-3
MDL number:	MFCD00284715
NACRES:	NA.52

Кат. номер

S9430-.5ML-PW

S9430-1ML-PW

S9430-1ML

S9430-.5ML

S9430-VAR

SYBR® Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.
SYBR® Green I nucleic acid gel stain has been used for real-time PCR quantification and flow cytometry.
SYBR® Green I nucleic acid gel stain has been used for:

• the quantification of dsDNA
• to stain DNA in polymerase chain reaction (PCR)
• for comet assay technique
• to assess spermatozoon membrane integrity

A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower (approximately 100 to 300 pg per band using 254 nm epi-illumination). SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.
SYBR is a registered trademark of Life Technologies

Quality Level	200
usage	1.0 mL sufficient for 100 mini-gels
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry.
concentration	10,000 x in DMSO
storage temp.	20°C

Personal Protective Equipment	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	201.2 °F - closed cup
Flash Point C	94 °C - closed cup