Protease from Streptomyces griseus

CAS Number:	9036-06-0
Enzyme Commission (EC) Number:	3.4.24.31 ( BRENDA | IUBMB )
EC Number:	232-909-5
MDL number:	MFCD00132092
eCl@ss:	32160410
NACRES:	NA.75

Кат. номер

P8811-100MG

P8811-5G

P8811-1G

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
100 mg in glass bottle
1, 5 g in glass bottle
An unusually non-specific protease.
Contains approximately 25% calcium acetate.
Completely inactivated by heating above 80 °C for 15-20 minutes.
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Collected from culture broth of S. griseus.

Quality Level	200
product line	BioReagent
form	powder
specific activity	3.5 units/mg solid
application(s)	cell culture | embryo: suitable
impurities	starch, essentially free
storage temp.	20°C

Pictograms	GHS07,GHS08
Signal Word	Danger
Hazard Statements	H315 - H319 - H334 - H335
Precautionary Statements	P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable