PSF-TEF1-COOH-BETAGAL - C-TERMINAL BETA GAL TAG YEAST PLASMID OGS2040-5UG

NACRES:

NA.85

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal Beta Galactosidase reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Analysis Note

form	buffered aqueous solution
mol wt	size 8364 bp
conjugate	beta-galactosidase tagged
Origin of replication	2Micron, pUC (500 copies)
Peptide cleavage	no cleavage
Peptide tag location	C-terminal
Promoter	Promoter name: TEF1 Promoter activity: constitutive Promoter type: yeast
bacteria selection	kanamycin
reporter gene	beta Gal
shipped in	ambient
storage temp.	20°C
yeast selection	uracil

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable