PSF-TEF1-COOH-3C-6HIS - C-TERMINAL 6 HIS TAG YEAST PLASMID OGS1923-5UG

NACRES:

NA.85

PSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid is a C-terminal 6His affinity tag yeast expression plasmid. This vector allows the creation of tagged proteins with a 3C cleavage tag.This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains a c-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.
About the Cleavage Tag:PSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname PreScission protease.
Promoter Expression Level: PSF-TEF1-COOH-3C-6HIS - C-terminal 6 HIS TAG yeast plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequence
Analysis Note

form	buffered aqueous solution
mol wt	size 6759 bp
conjugate	6-His tagged
Origin of replication	2Micron, pUC (500 copies)
Peptide cleavage	3C
Peptide tag location	C-terminal
Promoter	Promoter name: TEF1 Promoter activity: constitutive Promoter type: yeast
bacteria selection	kanamycin
reporter gene	none
shipped in	ambient
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable