PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID OGS185-5UG

NACRES:

NA.85

PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This plasmid also contains the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV) between the promoter and the multiple cloning site (MCS). It is designed to be used to express genes using eukaryotic T7 expression systems such as those used for the recovery of a variety of viruses. This vector is not designed for the expression of two proteins from the same mRNA. For this purpose we have designed pSF-CMV-MCS-IRES-PciI where the PciI site contains a start codon in the correct position for the IRES to express efficiently. In this vector there is an NcoI site (which contains an ATG start codon) in the correct position to enable efficient expression of a gene in the MCS.
The start codon that is within the NcoI restriction site is positioned to allow efficient expression from the ECMV IRES. The main MCS for gene insertions in this molecular cloning vector therefore extends from NcoI to XbaI. Downstream sites have alternate functions and are used to insert other DNA sequences that we sell however they can be used to insert a gene if these functions are not required.
The ClaI to NheI sites have other functions such as adding peptide tags second promoters or other IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
Sequence
Analysis Note

form	buffered aqueous solution
mol wt	size 4378 bp
Origin of replication	pUC (500 copies)
Peptide cleavage	no cleavage
Promoter	Promoter name: T7 Promoter activity: inducible Promoter type: phage
bacteria selection	kanamycin
reporter gene	none
shipped in	ambient
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable