PSF-OXB20-COOH-RLUC - C-TERMINAL RLUC TAG BACTERIAL PLASMID OGS3035-5UG

NACRES:

NA.85

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains a c-terminal Renilla luciferase reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
Sequence
Quick-reference Plasmid Map,
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File,
FASTA Vector Sequence File,
Full Plasmid Map,
Analysis Note
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics, - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
Oxford Genetics is a trademark of Oxford Genetics Ltd

form	buffered aqueous solution
mol wt	size 4787 bp
conjugate	Renilla luciferase conjugate
Origin of replication	pUC (500 copies)
Peptide cleavage	no cleavage
Peptide tag location	C-terminal
Promoter	Promoter name: OXB20 Promoter activity: constitutive Promoter type: bacterial
bacteria selection	kanamycin
reporter gene	Renilla luciferase
shipped in	ambient
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable