PSF-CMV-PURO-COOH-FRCFP - C-TERMINAL CFP TAG MAMMALIAN PLASMID OGS1366-5UG

NACRES:

NA.85

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal non-aequorea based cyan/blue fluorescent protein (FrCFP) reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Analysis Note
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form	buffered aqueous solution
mol wt	size 6855 bp
conjugate	cyan fluorescent protein (CFP) tagged
Origin of replication	pUC (500 copies)
Peptide cleavage	no cleavage
Peptide tag location	C-terminal
Promoter	Promoter name: CMV Promoter activity: constitutive Promoter type: mammalian
bacteria selection	kanamycin
mammalian cells selection	puromycin
reporter gene	CFP
shipped in	ambient
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable