Molecular Biology & Functional Genomics
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Histone Deacetylase 8 (HDAC8) Activity Assay Kit EPI006-1KT
Enzyme Commission (EC) Number: 3.5.1.98 ( BRENDA | IUBMB ) NACRES: NA.41 General descriptionHistone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.
With Sigmas HDAC8 Activity Assay Kit, HDAC8 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC8 activity. The kit provides a rapid, simple, sensitive, and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC8, and from native, recombinant, or genetically modified HDAC8.
HDAC8 (histone deacetylase 8) is a class I enzyme, that has 377 residues and it lies between class I and class II HDACs. This gene is located on X chromosome. It consists of a single α/β domain, in which eight parallel stranded β sheets are sandwiched between 13 α helices.
Biochem/physiol Actions
HDAC8 (histone deacetylase 8), with the help of PKA (cyclic AMP-dependent protein kinase A) plays a major role in confirming the acetylation state of histones. This protein may also plays an important role in acute myeloid leukemia (AML). Suppression of HDAC8 by RNA interference stops the growth of lung, colon, and cervical cancer cell lines.
Features and Benefits- Simple, sensitive, and reliable assay
- Utilizes fluorometric methods
- Sample type: cell and tissue lysates, plasma and serum, other biological fluids
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Suitable for high throughput measurement of HDAC8 activity in purified, immunoprecipitated, and recombinant or genetically modified HDAC8 samples
- Convenient 96-well microplate format
ПараметрыQuality Level 100 usage 100 assays in 96 well plates NCBI accession no. NM_001166418 (Human),","NM_027382 (Mouse), shipped in wet ice storage temp. 20°C Gene Information human ... HDAC8(55869)
mouse ... HDAC8(70315)
Safety InformationFlash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit EPI007-1KT
Enzyme Commission (EC) Number: 3.5.1.98 ( BRENDA | IUBMB ) NACRES: NA.41 General descriptionHistone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.
With Sigmas HDAC8 Inhibitor Screening Kit, HDAC8 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC8 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC8 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.
Features and Benefits- Simple, sensitive, and reliable assay
- Simple procedure; takes ~60 min
- Utilizes fluorometric methods
- Sample type: candidate HDAC8 inhibitors
- Suitable for screening HDAC8 inhibitors
- Suitable for individual tests or high throughput assays
- Convenient 96-well microplate format
ПараметрыQuality Level 100 usage 100 assays in 96 well plates NCBI accession no. NM_001166418 (Human),","NM_027382 (Mouse), shipped in wet ice storage temp. 20°C Gene Information human ... HDAC8(55869)
mouse ... HDAC8(70315)
Safety InformationFlash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Imprint® Chromatin Immunoprecipitation Kit
NACRES: NA.54
Кат. номер CHP1-96RXN CHP1-24RXN General descriptionImprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.
For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.ApplicationThe Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits- Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
- Sensitive—As few as 10,000 cells required for each ChIP sample
- Convenient—Fewest steps of any available ChIP protocol
- Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
- Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology
Legal InformationGenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 storage temp. 20°C
Safety Informationpictograms GHS02,GHS05,GHS07,GHS08 signalword Danger hcodes H225 - H290 - H302 - H315 - H319 - H334 - H336 - H412 pcodes P210 - P233 - P273 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 Target Organs Central nervous system RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 53.6 °F - closed cup Flash Point C 12 °C - closed cup Узнать цену -
Imprint® DNA Modification Kit
EC Number: 231-548-0 NACRES: NA.54
Кат. номер MOD50-1KT MOD50-50RXN General descriptionThe Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.ApplicationImprint® DNA Modification Kit has been used for bisulfite modification of DNA.Features and Benefits- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Storage and StabilityAll components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 usage sufficient for 50 reactions storage temp. 20-25°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H290 - H302 - H314 - H412 pcodes P234 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 Supp Hazards EUH031 RIDADR UN 3316 9 Узнать цену -
Imprint® Methylated DNA Quantification Kit
NACRES: NA.54
Кат. номер MDQ1-96RXN MDQ1-48RXN General descriptionImprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.ApplicationImprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.Features and Benefits- ELISA based format
- Procedure completes in 4 easy to follow steps
- All reagents supplied including control methylated DNA
- Detection limit is 5 ng of fully methylated DNA
- Input DNA may be as low as 10-200 ng
- Procedure completes in 3.5 hours
- 96 well format allows option of studying single samples or high-throughput studies
- Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
- May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200
Safety Informationpictograms GHS05 signalword Warning hcodes H290 - H412 pcodes P273 RIDADR UN 3316 9 Узнать цену -
Imprint® RNA Immunoprecipitation Kit RIP-12RXN
NACRES: NA.54 General descriptionRNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view workflow and application data.ApplicationImprint® RNA Immunoprecipitation Kit has been used for:- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200
Safety Informationpictograms GHS02,GHS07 signalword Warning hcodes H226 - H315 - H319 - H335 pcodes P261 - P305 + P351 + P338 RIDADR UN 3316 9 Flash Point F 100.4 °F Flash Point C 38 °C Узнать цену -
Imprint® Ultra Chromatin Immunoprecipitation Kit
Кат. номер CHP2-24RXN CHP2-48RXN CHP2-10RXN General descriptionThe Imprint Ultra Chromatin IP Kit, is Sigmas second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 µg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.Application- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits- Use successfully CHiPed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 x 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 x 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметры
Safety Informationpictograms GHS02,GHS03,GHS05,GHS07,GHS09 signalword Danger hcodes H226 - H271 - H302 - H314 - H336 - H400 pcodes P210 - P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 Target Organs Central nervous system RIDADR UN 3316 9 WGK Germany WGK 3 Узнать цену -
Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls CHP2NC-48RXN
General descriptionThe Imprint Ultra Chromatin IP Kit, is Sigmas second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 µg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.Application- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits- Use successfully CHiPed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 x 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 x 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety Informationpictograms GHS02,GHS03,GHS05,GHS07,GHS08,GHS09 signalword Danger hcodes H226 - H271 - H290 - H302 - H314 - H334 - H336 - H410 pcodes P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Central nervous system Узнать цену -
Imprint® Ultra Chromatin Optimization Kit CHROP-15RXN
NACRES: NA.54 General descriptionThe Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Components
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.Legal InformationImprint is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety Informationpictograms GHS02,GHS03,GHS05,GHS07,GHS09 signalword Danger hcodes H226 - H271 - H290 - H302 - H314 - H336 - H410 pcodes P210 - P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 Target Organs Central nervous system RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 100.4 °F Flash Point C 38 °C Узнать цену -
In Situ Histone Deacetylase (HDAC) Activity Fluorometric Assay Kit EPI003-1KT
EC Number: 200-664-3 NACRES: NA.41 General descriptionHistone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. HDACs are localized in both the cytosol and nucleus and some shuttle between the two locations.
Sigmas InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure HDAC activity in cultured cells. The procedure requires just two steps, both performed in the original 96-well cell culture plate. First, the cell culture medium is replaced with a cell permeable HDAC Substrate, containing an acetylated lysine side chain. During the subsequent incubation, HDAC Substrate enters the cells and is deacetylated by intracellular HDAC. In the second step, Developer is added to lyse the cells and cleave the deacetylated HDAC Substrate to release a fluorophore. The fluorescence generated can be quantified at Ex/Em = 368/442 nm. The assay is well suited for either individual or high throughput screening.ApplicationIn Situ Histone Deacetylase (HDAC) Activity Fluorometric Assay Kit has been used to determine HDAC activity.Biochem/physiol ActionsSite specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, histone deacetylases (HDACs) play a crucial role in organism development and disease. Mutation or abnormal expression of HDAC is observed in cancer.Features and Benefits- Simple two-step sensitive and reliable assay
- All steps performed in the same cell culture plate
- Utilizes fluorometric method
- Sample type: cultured, adherent, and suspension cells
- Suitable for individual tests or high throughput assays and kinetic studies
- Convenient 96-well microplate format
- Suitable for screening HDAC inhibitors or activators
- Suitable for studying growth factors or other regulators that influence HDAC activity
ПараметрыQuality Level 100 usage 100 assays in 96 well plates shipped in wet ice storage temp. 20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 - H412 pcodes P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Magna ChIP® Protein A Magnetic Beads
eCl@ss: 32160405
Кат. номер 16-661X 16-661 General descriptionRecombinant protein A covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein A agarose beads, protein A magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.ApplicationResearch Category
Epigenetics & Nuclear Function
Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
Used to detect/quantify: Protein AQualityRoutinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).Physical formLiquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.Storage and StabilityStable for 1 year at 2-8°C from date of shipment. Do Not Freeze.Legal InformationMAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLCDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 2 to 8 °C (Do not freeze) Quality Level 100 packaging pkg of 1 mL Торговая марка Magna ChIP® storage condition do not freeze particle size ~3 µm shipped in wet ice storage temp. 2-8°C Узнать цену -
Magna ChIP® Protein G Magnetic Beads
eCl@ss: 32160405
Кат. номер 16-662X 16-662 General descriptionRecombinant protein G covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein G agarose beads, protein G magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.ApplicationRecombinant Protein G covalently bound to magnetic beads for use in chromatin immunoprecipitations (ChIP assays). These protein G beads provide users a more rapid, reproducible & efficient reagent for collecting immunocomplexes vs. agarose beads.
Used to detect/quantify: Protein G
Research Category
Epigenetics & Nuclear Function
Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.QualityRoutinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).Physical formLiquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.Storage and StabilityStable for 1 year at 2-8°C from date of shipment. Do Not Freeze.Legal InformationMAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLCDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыstorage conditions 2 to 8 °C Quality Level 100 packaging pkg of 1 mL Торговая марка Magna ChIP® particle size ~3 µm shipped in wet ice storage temp. 2-8°C Узнать цену -
Magna ChIRP Human HOTAIR lncRNA Probe Set 3-312
eCl@ss: 32161000 General descriptionPervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
Quality
Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Physical form
10 mM Tris-HCl, 1mM EDTA, pH 8.0
Storage and Stability
Upon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100, Торговая марка Chemicon®, Magna ChIRP® application(s) DNA sequencing: suitable, RNA extraction: suitable (nuclear function kit), RNA purification: suitable (with magnetic beads), activity assay: suitable (protein interaction), protein purification: suitable (protein determination) Узнать цену -
Magna ChIRP Mouse XIST lncRNA Probe Set 3-311
eCl@ss: 32161000 General descriptionChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Mouse XIST lncRNA Probe Set contains 43 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of mouse lncRNA XIST. All probes are biotin-TEG-labeled at the 3-prime end. The oligonucleotides are split to two pools (even and odd) and are supplied ready for use in the ChIRP method. This probe set is fully compatible with the Maga ChIRP kits (cat. # 17-10494 and 17-10495).
Pervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
X inactivation is an early developmental process in mammalian females that transcriptionally silences one of the pair of X chromosomes, thus providing dosage equivalence between males and females. The process is regulated by several factors, including a region of chromosome X called the X inactivation center (XIC), which encodes the lncRNA XIST (X-inactive specific transcript). Reactivation of both X chromosomes XaXa, via XIST epigenetic silencing, has been identified as a hallmark of primed to nave stem cell conversion. Recently, using ChIRP–MS, Chu and colleagues captured 81 XIST-bound proteins in four different types of mouse embryonic stem cells during X chromosome inactivation.Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 100 Торговая марка Chemicon®","Magna ChIRP® application(s) DNA sequencing: suitable","RNA extraction: suitable (nuclear function kit)","RNA purification: suitable (with magnetic beads)","activity assay: suitable (protein interaction)","detection: suitable (RNA)","protein purification: suitable (protein determination) NCBI accession no. NR_001463, Узнать цену -
Magna ChIRP U1snRNA Probe 3-313
eCl@ss: 32161000 General descriptionPervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
Quality
Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Physical form
10 mM Tris-HCl, 1mM EDTA, pH 8.0
Storage and Stability
Upon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Chemicon®","Magna ChIRP® application(s) DNA sequencing: suitable","RNA extraction: suitable (nuclear function kit)","RNA purification: suitable (with magnetic beads)","activity assay: suitable (protein interaction)","detection: suitable (RNA)","protein purification: suitable (protein determination) NCBI accession no. NR_004430, Узнать цену -
Magna ChIRP U2snRNA Probe 3-314
eCl@ss: 32161000 General descriptionPervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP® RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
The Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1. The probe is biotin-TEG-labeled at the 3-prime end. The oligonucleotide is supplied ready for use in the ChIRP method.ApplicationThe Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1.
Quality
Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Physical form
10 mM Tris-HCl, 1mM EDTA, pH 8.0Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationMAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Magna ChIRP® application(s) DNA sequencing: suitable","RNA extraction: suitable (nuclear function kit)","RNA purification: suitable (with magnetic beads)","activity assay: suitable (protein interaction)","detection: suitable (RNA) NCBI accession no. NR_002716, Узнать цену -
MS-275
Empirical Formula (Hill Notation): C21H20N4O3 CAS Number: 209783-80-2 Molecular Weight: 376.41 MDL number: MFCD03453552 PubChem Substance ID: 329799578 NACRES: NA.41
Кат. номер EPS002-5MG EPS002-1MG General descriptionPreferentially inhibits HDAC1 (IC50=300 nM) over HDAC3 (IC50=8 µM). Has no inhibitory activity towards HDAC8 (IC50>100 µM).ApplicationMS-275 has been used as a histone deacetylase-1 inhibitor.Biochem/physiol ActionsHDAC inhibitor; antiproliferative.ПараметрыQuality Level 100 assay 99% solubility DMSO: 38 mg/mL storage temp. 20°C SMILES string Nc1ccccc1NC(=O)c2ccc(CNC(=O)OCc3cccnc3)cc2 InChI 1S/C21H20N4O3/c22-18-5-1-2-6-19(18)25-20(26)17-9-7-15(8-10-17)13-24-21(27)28-14-16-4-3-11-23-12-16/h1-12H,13-14,22H2,(H,24,27)(H,25,26) InChI key INVTYAOGFAGBOE-UHFFFAOYSA-N Gene Information human ... HDAC1(3065), HDAC10(83933), HDAC11(79885), HDAC2(3066), HDAC3(8841), HDAC4(9759), HDAC5(10014), HDAC6(10013), HDAC7(51564), HDAC8(55869), HDAC9(9734)
Safety Informationpictograms GHS06,GHS08 signalword Danger hcodes H301 - H360 pcodes P201 - P301 + P310 + P330 RIDADR UN 2811 6.1 / PGIII WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SDS Lysis Buffer - for use in ChIP Assay 20-163
eCl@ss: 32160405 NACRES: NA.41 ApplicationResearch Category
All
For use in Chromatin Immunoprecipitation assays.Physical form1% SDS, 10mM EDTA and 50mM Tris, pH 8.1Storage and Stability1 year at room temperatureLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, GermanyDisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100 Торговая марка Upstate® shipped in ambient Узнать цену -
SIRT6 Inhibitor Screening Kit (Fluorometric) EPI017-1KT
General descriptionSIRT6 or Sirtuin 6 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. SIRT6 is a nuclear sirtuin that has been associated with aging, cellular protection, sugar metabolism and certain types of cancer. Broad therapeutic applications are foreseen for SIRT6 inhibitors, including uses in diabetes, immune-mediated disorders, and cancer. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for screening, characterizing and studying SIRT6 inhibitors
Principle
In this Sirtuin 6 inhibitor screening kit, Sirtuin 6 deacetylates the substrate, followed by cleavage of the deacetylated substrate to release the fluorescent group, which is detected fluorometrically (λex = 400 nm/λem = 505 nm).ПараметрыQuality Level 100, usage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS07,GHS08 signalword Danger hcodes H302 + H312 - H315 - H317 - H318 - H334 - H361d - H412 pcodes P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SIRTUIN 1 ELISA KIT EPI015-1KT
EC Number: 259-364-6 ApplicationFor research use only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315-H319-H335 pcodes P261-P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену -
Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit EPI010-1KT
NACRES: NA.41 General descriptionSirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins. Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules. The gene encoding this protein is localized on human chromosome 19q13.2.
With Sigmas Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.ApplicationSirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay.
Biochem/physiol Actions
Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntingtons disease. Huntingtons disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of -tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.
Features and Benefits- Simple, sensitive, and reliable assay
- Species reactivity: mammalian
- Utilizes fluorometric methods
- Suitable for individual tests or high throughput assays
- Convenient 96-well microplate format
- Screening and characterization of Sirtuin inhibitors
Параметрыusage 100 assays in 96 well plates storage condition protect from light NCBI accession no. NM_001122765 (Mouse),","NM_001193286 (Human), shipped in wet ice storage temp. 20°C Gene Information human ... SIRT2(22933)
mouse ... SIRT2(64383)
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 Узнать цену -
Sirtuin Activity Assay Kit (Fluorometric) EPI018-1KT
NACRES: NA.84 General descriptionSirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.SuitabilitySuitable for the detection of Sirtuin Activity in purified recombinant protein, cell and tissue lysate, nuclear extract, mitochondria and immunoprecipitated samplesPrincipleIn the Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically ((ex = 400/em = 505 nm). HDACs also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDACs in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay
is 0.06 µU of recombinant human SIRT6.Параметрыusage sufficient for 100 fluorometric tests detection method fluorometric storage temp. −20°C
Safety Informationpictograms GHS05,GHS08 signalword Danger hcodes H317 - H318 - H334 pcodes P261 - P272 - P280 - P284 - P302 + P352 - P305 + P351 + P338 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TE Buffer - for use in ChIP Assays 20-157
eCl@ss: 32160405 NACRES: NA.41 ApplicationResearch Category
All
For use in Chromatin Immunoprecipitation assays.
Physical form
10mM Tris-HCl, pH 8.0, 1mM EDTA
Storage and Stability
1 year at 4°CLegal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.ПараметрыQuality Level 100, Торговая марка Upstate® shipped in wet ice Узнать цену -
CAS9 Blasticidin Lenti Particles LVCAS9BST-1EA
NACRES: NA.51 General descriptionReady-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Blasticidin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Blast using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.ApplicationFunctional Genomics/Screening /Target Validation/Genome EditingComponents200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.LinkageClick here to order gRNA plasmid and virus.ПараметрыQuality Level 200 packaging vial of 200 µL concentration 1 x 10 6 VP/ml virus (via p24 assay) selection blasticidin shipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CAS9 Blasticidin Lenti Plasmid CAS9BST-1EA
NACRES: NA.51 General descriptionThis product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigmas lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigmas lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.ApplicationFunctional Genomics/Screening /Target Validation/Genome EditingComponents1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin LentiLinkageClick here to order gRNA plasmid and virus.ПараметрыQuality Level 200 recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) selection ampicillin, blasticidin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 Geminin plasmid CAS9GEMP-1UG
General descriptionThis product is an expression plasmid that utilizes the EF1a promoter for strong transient expression of a Cas9-GFP-Geminin fusion (EF1a-Cas9-GFP-Geminin) allowing for easy visualization of successful transfection. The Cas9-Geminin expression plasmid is one part of a two part CRISPR system with individual Cas9-Geminin and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Performing HDR mediated targeted integration in multiple cell lines
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Features and Benefits- Highly specific and highly active
- Sequence verified
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. While HDR is absent in G1, NHEJ is active throughout the cell cycle and is largely favored over HDR. Consequently HDR can be increased by directly synchronizing the expression of Cas9 with cell-cycle progression by fusing Cas9 to human Geminin (a protein expressed in S and G2 phases). The Cas9-geminin fusion protein then regulates gene editing by promoting repair during S and G2 phases when homology directed repair (HDR) occurs. The result is creation of double strand breaks in cells at times that are more able to incorporate donor sequences.Legal InformationCRISPR Label License,Параметрыrecombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) application(s) CRISPR: suitable Promoter Promoter name: EF1-alpha reporter gene GFP selection ampicillin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 GFP Lentiviral Particles
General descriptionReady-to-use Cas9-GFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-GFP lentiviral particles efficiently and stably integrate Cas9 and GFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of cell lines stably expressing Cas9-GFP
Features and Benefits- Highly specific
- Highly active
- Ready to infect/transduce
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Unit Definition
VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.Legal InformationCRISPR Use License Agreement,
Evrogen Fluorophore Licenses,
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Cas9 Hygromycin Lenti Plasmid CAS9HYGROVP-1UG
General descriptionThis product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a hygromycin resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Hygro) allowing for easy visualization of successful transfection or transduction. Use Sigmas lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigmas Cas9-Hygro lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Manufacture of Cas9 expressing Lentiviral Particles
Features and Benefits- Highly specific and highly active
- Sequence verified
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Legal InformationCRISPR Label License,
Lentiviral, WPRE, and Evrogen Licenses,Параметрыrecombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) application(s) CRISPR: suitable selection blasticidin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 Hygromycin Lentiviral Particles
General descriptionReady-to-use Cas9-Hygro lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-Hygro lentiviral particles efficiently and stably integrate Cas9 and a hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-Hygro) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of cell lines stably expressing Cas9
Features and Benefits- Highly specific and highly active
- Sequence verified
- Ready to infect/transduce
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Unit Definition
VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.Legal InformationCRISPR Label License,
Lentiviral, WPRE, and Evrogen Licenses,
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Cas9 mRNA
Кат. номер CAS9MRNA-1EA CAS9MRNA-1SET General descriptionCas9 mRNA generated through in-vitro transcription which has been capped and polyA-tailed. This mRNA should be used in conjunction with purified guideRNA. The use of RNA vs. plasmid constructs avoids complications with promoter-embryo compatibility as well as the possibility of random integration of nuclease and gRNA-expressing plasmids into the host animal genome.ApplicationFunctional Genomics/Transgenic Applications- Creation of gene knockouts in transgenic applications
- Creation of knock-in animals with promoters, fusion tags or reporters integrated into endogenous genes
Features and BenefitsRNA ready to use in transgenic applications. Must be used in conjunction with a purified guideRNA sequence to mediate a site specific double strand break in the DNA.Components1 vial containing 25ug of Cas9 mRNA.
Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Physical formSigma Cas9 mRNA is supplied at a concentration of 500 ng/ul (50 ul Cas9 mRNA, capped and polyA-tailed)Preparation NoteWhile Sigma implements the same high quality RNA synthesis procedures that have been successful in many mRNA-based ZFN transgenic projects, we highly recommend centrifuging your final, concentration-adjusted CRISPR RNA samples immediately prior to microinjection to ensure all particulate material has been removed which may result in clogging of microinjection needles.Other NotesTypical microinjection concentrations used in the literature are in the ranges of 20-200 ng/ul for Cas9 mRNA and 10-50 ng/ul for gRNA.
Must be used in conjunction with the RNA format of a guideRNA sequence in order to mediate a double strand break in the DNA.
While Sigma CRISPR RNA was not developed for robust application to cell culture, we have seen some success in detecting CEL-I activity using RNA-only formats for both CRISPR nucleases and paired nickases. RNA-only delivery formats may be favorable for cell types which are sensitive to double stranded DNA such a dendritic cells or when promoter-cell incompatibilities exist.Legal InformationCRISPR Use License AgreementПараметрыQuality Level 200 packaging vial of 50 μL concentration 500 ng/μL shipped in dry ice storage temp. −70°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 Neomycin Lenti Particles LVCAS9NEO-1EA
NACRES: NA.51 General descriptionReady-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.ApplicationFunctional Genomics/Screening /Target Validation/Genome Editing
Components
200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.LinkageClick here, to order gRNA plasmid and virus.Параметрыpackaging vial of 200 µL Quality Level 200, concentration 1 x 10 6 VP/ml virus (via p24 assay) application(s) CRISPR: suitable selection neomycin / G418 shipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3245 9 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 Neomycin Lenti Plasmid CAS9NEO-1EA
NACRES: NA.51 General descriptionThis product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigmas lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigmas lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.ApplicationFunctional Genomics/Screening /Target Validation/Genome Editing
Features and Benefits
Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.
Components
1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin LentiLinkageClick here, to order gRNA plasmid and virus.ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) application(s) CRISPR: suitable selection blasticidin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 plasmid
NACRES: NA.51
Кат. номер CAS9P-1EA CAS9P-1SET General descriptionThe Cas9 expression plasmids use the CMV promoter for strong transient expression of Cas9. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9 expression plasmids can be linearized using XbaI for T7-based mRNA production.ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Components1 vial containing 1ug of Cas9 plasmid.
Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Physical formSigma Cas9 plasmid DNA is supplied at concentrations of 20ng/ul in 50ul.Preparation NoteSigma CRISPR plasmid products are delivered as mini-prep aliquots, which may not be suitable for transfection into particular cell types. For best results, we advise maxi-prepping plasmids using endotoxin-free DNA purification kits prior to transfection.Other NotesMust be used in conjunction with a U6-gRNA plasmid in order to mediate a double strand break in the DNA.
Typical transfection concentrations used in literature are in the ranges of >= 1.0 ug/uL and <= 5 uL of Cas9 plasmid combined with >= 1.0 ug/uL and <= 5 uL of U6-gRNA plasmids. (All dosages above assume 0.5 to 1 million cells nucleofected)Legal InformationCRISPR Use License AgreementПараметрыQuality Level 200 recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV selection kanamycin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 Protein
Кат. номер CAS9PROT-250UG-PW CAS9PROT-50UG-PW CAS9PROT-50UG CAS9PROT-4X250UG CAS9PROT-250UG CAS9PROT-2X250UG General descriptionCAS9, also known as cas5 and csn1, is the signature gene of the type II clustered regularly interspaced short palindromic repeats (CRISPR)-RuvC (RNase H-like fold) cas system. CAS9 contains 1388 amino acids. This protein is predicted to contain a RuvC/ ribonuclease (RNase) H domain involved in crRNA maturation and McrA/HNH signature domain involved in the DNA degradation step.
Recombinant Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, wild type Streptococcus pyogenes Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.ApplicationFunctional Genomics/Target Validation/Genome EditingBiochem/physiol ActionsCAS9 plays a vital role in plasmid DNA interference. It is the only cas protein needed to deliver resistance against foreign DNA. CAS9 stimulates both RNA-guided genome editing and gene regulation in various organisms, but it can facilitate only one activity at a time within any given cell.Features and Benefits- Highly specific
- Highly active
- Ready-to-inject/transfect
Packagingpkg of 50 µg ( 300 pmol)
pkg of 250 µg ( 1500 pmol)ComponentsEach kit consists of:- one vial of Cas9 recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.ReconstitutionLyophilized S. pyogenes Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.Other NotesUse our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteinsLegal InformationCRISPR Use License Agreement
MISSION is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 300 recombinant expressed in E. coli assay 95% (SDS-PAGE) form lyophilized powder packaging pkg of 1 kit (3 components) application(s) CRISPR: suitable shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9 RFP Lentiviral Particles
General descriptionReady-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of cell lines stably expressing Cas9-RFP
Features and Benefits- Highly specific
- Highly active
- Ready to infect/transduce
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Unit Definition
VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.Legal InformationCRISPR Label License,
Lentiviral, WPRE, and Evrogen Licenses,
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Cas9 Zeo Lenti Plasmid CAS9ZEOVP-1UG
General descriptionThis product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a Zeo resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Zeo) allowing for easy visualization of successful transfection or transduction. Use Sigmas lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigmas Cas9-Zeo lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Manufacture of Cas9 expressing Lentiviral Particles
Features and Benefits- Highly specific
- Highly active
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Legal InformationCRISPR Label License,
Lentiviral, WPRE, and Evrogen Licenses,Параметрыrecombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) application(s) CRISPR: suitable selection blasticidin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9-D10A Nickase Protein
NACRES: NA.51
Кат. номер CAS9D10APR-250UG-P CAS9D10APR-50UG CAS9D10APR-250UG CAS9D10APR-50UG-PW General descriptionRecombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific paired guide RNAs, S. pyogenes Cas9-D10A nickase will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Cas9 (CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein-9 nuclease) nickase creates double-stranded breaks through two nuclease domains, named RuvC and HNH.ApplicationFunctional Genomics/Target Validation/Genome EditingFeatures and Benefits- Highly specific
- Highly active
- Ready-to-inject/transfect
Packagingpkg of 50 µg ( 300 pmol)
pkg of 250 µg ( 1500 pmol)ComponentsEach kit consists of:- one vial of Cas9-D10A Nickase recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.ReconstitutionLyophilized S. pyogenes Cas9-D10A Nickase protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.Other NotesUse our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
See the product in our recent article on bioRxiv Highly efficient and specific genome editing in human cells with paired CRISPR-Cas9 nickase ribonucleoproteinsLegal InformationCRISPR Use License Agreement
MISSION is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 assay 95% (SDS-PAGE) form lyophilized powder packaging pkg of 1 kit (3 components) shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Cas9-GFP Lenti Plasmid CAS9GFPVP-1UG
General descriptionThis product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and GFP linked by a 2A peptide (EF1a-Cas9-2A-GFP) allowing for easy visualization of successful transfection or transduction. Use Sigmas lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigmas Cas9-GFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Manufacture of Cas9-GFP expressing Lentiviral Particles
Features and Benefits- Highly specific
- Highly active
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Legal InformationCRISPR Use License Agreement,
Lentiviral, WPRE and Evrogen Fluorophore Licenses,Параметрыrecombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) reporter gene GFP selection ampicillin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Cas9-GFP Protein
Кат. номер CAS9GFPPRO-250UG CAS9GFPPRO-50UG CAS9GFPPRO-4X250UG CAS9GFPPRO-2X250UG General descriptionRecombinant Cas9-GFP protein from Streptococcus pyogenes (~194 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, wild type Streptococcus pyogenes Cas9-GFP protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
An N-terminally fused enhanced green fluorescent protein with an excitation peak at 488 nm and emission peak at 509 nm allows for visualization of transfected RNP complex in addition to utility in flow cytometry applications. The protein also contains three varied nuclear localization sequences postioned for optimal activity.Application- Functional Genomics
- Target Validation
- Genome Editing
- Fluorescence Microscopy
- Flow Cytometry
Features and Benefits- Highly specific
- Highly active
- Ready-to-inject/transfect
Packaging- pkg of 50 µg( 260 pmol )
- pkg of 250 µg( 1300 pmol )
ComponentsEach kit consists of- one vial of lyophilzed Cas9-GFP recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.ReconstitutionLyophilized S. pyogenes Cas9-GFP protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.Other NotesUse our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteinsLegal InformationCRISPR Use License Agreement
MISSION is a registered trademark of Sigma-Aldrich Co. LLCПараметрыrecombinant expressed in E. coli assay 90% (SDS-PAGE) form lyophilized powder packaging pkg of 1 kit (3 components) reporter gene GFP shipped in wet ice storage temp. 20°C
Safety InformationWGK Germany WGK 3 Узнать цену -
Cas9-RFP Lenti Plasmid CAS9RFPVP-1UG
General descriptionThis product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and RFP linked by a 2A peptide (EF1a-Cas9-2A-RFP) allowing for easy visualization of successful transfection or transduction. Use Sigmas lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigmas Cas9-RFP lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed when packaged into lentiviral particles.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Manufacture of Cas9-RFP expressing Lentiviral Particles
Features and Benefits- Highly specific
- Highly active
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Legal InformationCRISPR Use License Agreement,
Lentiviral, WPRE and Evrogen Fluorophore Licenses,Параметрыrecombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of Plasmid DNA) application(s) CRISPR: suitable reporter gene RFP selection ampicillin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-CAS9-2A-GFP Plasmid CAS9GFPP-1EA
NACRES: NA.51 General descriptionThe Cas9 expression plasmids use the CMV promoter for strong transient expression of Cas9. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9 expression plasmids can be linearized using XbaI for T7-based mRNA production. The addition of a fluorophore that is translationally co-expressed with the Cas9 nuclease allows for easy visualization of successful transfection.ApplicationCMV-CAS9-2A-GFP plasmid has been used to induce additional sex combs-like1 mutations in human U937 leukemic cells. It has also been used in CRISPR/Cas9 analysis.
CMV-CAS9-2A-GFP plasmid is suitable for functional genomics/target validation for:- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Components1 vial containing 1 µg of Cas9-2A-GFP plasmid.
Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.PrincipleCRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.Other NotesMust be used in conjunction with a U6-gRNA plasmid in order to mediate a double strand break in the DNA.
Typical transfection concentrations used in literature are in the ranges of >= 1.0 µg/µL and <= 5 µL of Cas9 plasmid combined with >= 1.0 µg/µL and <= 5 µL of U6-gRNA plasmids. (All dosages above assume 0.5 to 1 million cells nucleofected).Legal InformationCRISPR Use License AgreementПараметрыQuality Level 200 recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV reporter gene GFP selection kanamycin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-CAS9-2A-RFP Plasmid CAS9RFPP-1EA
NACRES: NA.51 General descriptionThe Cas9 expression plasmids use the CMV promoter for strong transient expression of Cas9. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9 expression plasmids can be linearized using XbaI for T7-based mRNA production. The addition of a fluorophore that is translationally co-expressed with the Cas9 nuclease allows for easy visualization of successful transfection.ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Components
1 vial containing 1 µg of Cas9-2A-RFP plasmid.
Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Physical form
Sigma Cas9-2A-RFP plasmid DNA is supplied at concentrations of 50ng/μl in 20μl.Other NotesMust be used in conjunction with a U6-gRNA plasmid in order to mediate a double strand break in the DNA.
Typical transfection concentrations used in literature are in the ranges of >= 1.0 µg/µL and <= 5 µL of Cas9 plasmid combined with >= 1.0 µg/µL and <= 5 µL of U6-gRNA plasmids. (All dosages above assume 0.5 to 1 million cells nucleofected).Legal InformationCRISPR Use License Agreement,ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV reporter gene RFP shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-CAS9D10A-2A-GFP Plasmid CAS9D10AGFPP-1EA
NACRES: NA.51 General descriptionThe Cas9-D10A nickase plasmid co-expressing GFP uses the CMV promoter for strong transient expression of Cas9-D10A. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production.ApplicationCMV-CAS9D10A-2A-GFP Plasmid has been used in CRISPR-Cas9 mutagenesis to truncate the forkhead box O3 (FOXO3) gene in the mammalian cell.
Functional Genomics
Use of Paired Cas9 Nickase + GFP for:- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Components
1 vial containing 1ug of Cas9-D10A Nickase-GFP plasmid.
Please note, this product does not contain any guide RNA sequence. A pair of gRNA plasmids must be purchased separately through the Custom CRISPR paired nickase product tab.
Physical form
1 ug of Sigma Cas9-D10A nickase-GFP plasmidOther NotesThe type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. GFP is co-expressed from the same mRNA as the Cas9 nickase protein via a 2A peptide linkage, enabling tracking of transfection efficiency and enrichment of genome editing activity in cell populations via fluorescence activated cell sorting (FACS).Legal InformationCRISPR Use License Agreement,ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV reporter gene GFP selection kanamycin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-CAS9D10A-2A-RFP Plasmid CAS9D10ARFPP-1EA
NACRES: NA.51 General descriptionThe Cas9-D10A nickase plasmid co-expressing RFP uses the CMV promoter for strong transient expression of Cas9-D10A. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production.ApplicationFunctional Genomics
Use of Paired Cas9 Nickase + RFP for:- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Components
1 vial containing 1ug of Cas9-D10A Nickase-RFP plasmid.
Please note, this product does not contain any guide RNA sequence. A pair of gRNA plasmids must be purchased separately through the Custom CRISPR paired nickase product tab.
Physical form
2 ug of Sigma Cas9-D10A nickase-RFP plasmidOther NotesThe type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. RFP is co-expressed from the same mRNA as the Cas9 nickase protein via a 2A peptide linkage, enabling tracking of transfection efficiency and enrichment of genome editing activity in cell populations via fluorescence activated cell sorting (FACS).Legal InformationCRISPR Use License Agreement,ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV reporter gene RFP selection kanamycin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-ESPCAS9-2A-GFP Plasmid ESPCAS9GFP-1EA
General descriptionThis product is an expression plasmid that utilizes the CMV promoter for strong transient expression of eSpCas9 and GFP linked by a 2A peptide (CMV-eSpCas9-2A-GFP) allowing for easy visualization of successful transfection. The eSpCas9 expression plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Features and Benefits- Enhanced specificity compared to wild type Cas9
- Highly Active
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.Legal InformationCRISPR Use License Agreement,
Evrogen Fluorophore Licenses,ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CMV-ESPCAS9-2A-RFP Plasmid ESPCAS9RFP-1EA
General descriptionThis product is an expression plasmid that utilizes the CMV promoter for strong transient expression of eSpCas9 and RFP linked by a 2A peptide (CMV-eSpCas9-2A-RFP) allowing for easy visualization of successful transfection. The eSpCas9 expression plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors.
To order gRNA in any format click here,ApplicationFunctional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Features and Benefits- Enhanced specificity compared to wild type Cas9
- Highly active
- Ready to use purified plasmid DNA
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.Legal InformationCRISPR Use License Agreement,
Evrogen Fluorophore Licenses,ПараметрыQuality Level 200, recombinant expressed in E. coli packaging vial of 50 µL concentration 20 ng/µL in TE buffer; DNA (1µg of plasmid DNA) Promoter Promoter name: CMV reporter gene RFP selection kanamycin shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CompoZr™ Custom Zinc Finger Nuclease (ZFN) CSTZFND-1KT
General descriptionZinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cells natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.ApplicationThe CompoZr Custom ZFN service can be used to target genes from a variety of organisms. Validation of the custom ZFNs is completed in a proxy cell line for human, mouse, rat and hamster (CHO) projects. In this assay, the candidate ZFNs are delivered to the proxy cell line and activity is identified and measured. Activity is measured by amplifying over the ZFN target site and then using a nuclease mismatch enzyme to cut DNA strands that have been modified. ZFN efficiency can be measured based on the densitometry results of this assay. CompoZr Custom ZFN Projects for human, mouse, rat, and hamster (CHO) will have a production timeline of 10 weeks after the ZFN design is approved.
Functional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
- Creation of cell lines that produce higher yields of proteins or antibodies
Components- Best Performing ZFN Pair
- ZFN Pair in Plasmid Form
- Forward and Reverse Primers that allow for determination of rate of mutation and for screening of individual clones
- Positive Control DNA
- Used to determine a baseline cutting efficiency
Legal InformationAll Zinc Finger Nucleases are sold under license from Sangamo Biosciences. 3D Insert is a trademark of 3D Biotek, LLC
CompoZr is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, product line CompoZr® shipped in dry ice storage temp. 70°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CompoZr® Custom Zinc Finger Nuclease (ZFN)
Кат. номер CSTZFN2 CSTZFN-1KT CSTZFNY2 CSTZFN General descriptionZinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cells natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.ApplicationThe CompoZr Custom ZFN service can be used to target genes from a variety of organisms. Validation of the custom ZFNs is completed in a proxy cell line for human, mouse, rat and hamster (CHO) projects. In this assay, the candidate ZFNs are delivered to the proxy cell line and activity is identified and measured. Activity is measured by amplifying over the ZFN target site and then using a nuclease mismatch enzyme to cut DNA strands that have been modified. ZFN efficiency can be measured based on the densitometry results of this assay. CompoZr Custom ZFN Projects for human, mouse, rat, and hamster (CHO) will have a production timeline of 10 weeks after the ZFN design is approved.
Functional Genomics/Target Validation- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
- Creation of cell lines that produce higher yields of proteins or antibodies
Features and Benefits- Rapid design, assembly, and validation of a ZFN pair targeting your gene of interest
- Rapid and permanent disruption of, or integration into, any genomic loci
- Mutations made are permanent and heritable
- Works in a variety of mammalian somatic cell types
- Edits induced through a single transfection experiment
- Knockout or knock-in cell lines in as little as two months
- Single or biallelic edits occur in 1-20% of clone population
- No antibiotic selection required for screening
Components- Best Performing ZFN Pair
- 10 Aliquots of Ready-to-Deliver ZFN Pair in mRNA form
- ZFN Pair in Plasmid Form
- Forward and Reverse Primers that allow for determination of rate of mutation and for screening of individual clones
- Positive Control DNA
- Used to determine a baseline cutting efficiency
Other NotesTo have a ZFN made targeting your gene of interest, please inquire about our
Custom ZFN Service offering HERE,Legal InformationAll Zinc Finger Nucleases are sold under license from Sangamo Biosciences. CompoZr is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, product line CompoZr® shipped in dry ice storage temp. 70°C
Safety InformationRIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
