Molecular Biology & Functional Genomics
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PSF-CMV-UB ASCI - DUAL PROMOTER EXPRESSION PLASMID OGS399-5UG
NACRES: NA.85 General descriptionThis plasmid contains two promoters and two multiple cloning sites for the dual expression of transgenes. The first multiple cloning site allows for the expression of a transgene in mammalian cells under the control of the CMV promoter. The second multiple cloning site is downstream of the human Ubiquitin (Ub) promoter for expression in mammamalian cells. The second promoter (Ub) is approximately 8-10 fold weaker than the CMV promoter in most commonly used cell lines by transient transfection.
Promoter Expression Level:ApplicationFirst multiple cloning site notes: There is start codon in the NcoI site that can be removed by digestion with KpnI if required. The first MCS for gene insertions extends from NotI to XbaI but the Shine-Dalgarno sequences and Kozak sequences are aligned with the start codon in the NcoI restriction site. The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
Second multiple cloning site notes: The second MCS in this plasmid extends from PspOMI to SpeI. This MCS contains a Kozak sequence immediately upstream of the start codon in the PciI restriction site. We insert the start codons of our genes into here where possible.
This MCS has been designed to be compatible with genes that are within our main MCS by using enzymes sites in the same order that produce compatible cohesive ends that can be ligated together. This allows genes to be transferred from the main MCS (NotI to NheI) into the second MCS (PspOMI to SpeI) is required. Enzymes that are compatible between these two MCSs include:<UL><LI>PspOMI compatible with NotI
ScaI compatible with EcoRV (Blunt)
SalI compatible with XhoI
PciI compatible with NcoI and BspHI
AclI compatible with ClaI
SpeI compatible with XbaI and NheI Avr2</li></ul>
Sequence
Analysis NoteПараметрыform buffered aqueous solution Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-BETAGAL ASCI - BETA GALACTOSIDASE PLASMID OGS158-5UG
NACRES: NA.85 General descriptionThis vector contains a beta galactosidase reporter gene expression cassette under the control of the human Ubiquitin promoter that is designed as an add-on to any of our vectors. The Ub luciferase cassette is flanked by AscI sites but is not designed to be split up. If you would like the luciferase gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus promoter driven vector in the same format if you require an expression vector with lower levels of reporter gene activity.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 8694 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter type: mammalianbacteria selection kanamycin reporter gene beta Gal shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-BLAST ASCI - UBIQUITIN PROMOTER BLASTICIDIN PLASMID OGS384-5UG
NACRES: NA.85 General descriptionThis vector contains a blasticidin (Blast) resistance expression cassette under control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our vectors. The entire Ub Blast cassette is flanked by AscI sites. The Ub promoter is approximately 10-fold weaker than CMV. We also have have a weaker Rous Sarcoma Virus LTR promoter driven vector (pSF-CMV-RSV-Blast AscI) in the same format if you require even lower levels of blasticidin resistance.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5981 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection blasticidin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-BLAST-SV40 ORI SBFI - BLASTICIDIN / SV40 ORI PLASMID OGS410-5UG
NACRES: NA.85 General descriptionThis vector contains a Blasticidin (Blast) resistance expression cassette under the Ub promoter. It also contains the Simian Virus 40 (SV40) origin of replication. This allows the plasmid to be amplified in cells containing the SV40 large T antigen. This can be used to increase transgene signal following transient transfection. It is also possible to make cell lines containing the plasmid as a multi-copy episome. We have validated this activity in 293T cells. The entire Ub Blast cassette is flanked by AscI sites. This vector can also be used to force recombination into the genome of mammalian cells not expressing SV40 large T antigen following transfection and Blasticidin selection.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6171 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection blasticidin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-FLUC ASCI - FIREFLY LUCIFERASE PLASMID OGS155-5UG
NACRES: NA.85 General descriptionThis plasmid contains a luciferase cassette under the control of the Ub promoter that is designed as an add-on to any of our other plasmids. The Ub luciferase cassette is flanked by AscI sites but is not designed to be split up. If you would like the luciferase gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus promoter driven plasmid in the same format if you require an expression vector with lower levels of reporter gene activity.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 7200 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-FRCFP - MAMMALIAN CFP REPORTER VECTOR OGS512-5UG
NACRES: NA.85 General descriptionA CMV expression plasmid that allows for the insertion of exogenous transgenes for expression in mammalian cells. The plasmid also contains a reporter gene cassette away from the multiple cloning site that allows for high levels of expression of Cyan Fluorescent protein. The reporter in this plasmid was original developed by DNA2. 0 and is called Frosty CFP.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6263 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene CFP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-HYGRO ASCI - HYGROMYCIN SELECTION PLASMID OGS20-5UG
General descriptionThis plasmid contains a Hygromycin (Hygro) resistance expression cassette under the Ub promoter that is designed as an add-on to any of our other plasmids. The Ub Hygro cassette is flanked by AscI sites but is not designed to be split up. If you would like the Hygromycin gene alone or the Ub promoter alone please see the mammalian selection gene section or the promoter section on our website. This plasmid can be used to force recombination into the genome of mammalian cells following transfection and selection although this method can be inefficient. It can also be used conjunction with the SV40 origin of replication in cells expressing the SV40 large T antigen (such as 293T cells) to make stable cells lines following transfection and selection.
Promoter Expression Level: This plasmid vector contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
PSF-CMV-UB-HYGRO ASCI-hygromycin selection plasmid having the hygromycin resistance gene, which is driven by the ubiquitin promoter away from the CMV expression cassette.ApplicationCloning in a gene: PSF-CMV-UB-HYGRO ASCI-hygromycin selection plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6581 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection hygromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-HYGRO-SV40 ORI SBFI - HYGROMYCIN / SV40 ORI PLASMID OGS135-5UG
NACRES: NA.85 General descriptionThis vector contains a Hygromycin (Hygro) resistance expression cassette under the Ub promoter. It also contains the Simian Virus 40 (SV40 origin of replication. This allows the plasmid to be amplified in cells containing the SV40 large T antigen. This can be used to increase transgene signal following transient transfection. It is also reported that it is possible to make cell lines containing the plasmid as a multi-copy episome however we have not validated it for this purpose. The Ub Hygro cassette is flanked by AscI sites in this plasmid and is not designed to be split up. If you would like the Hygromycin gene alone or the Ub promoter alone please see the mammalian selection gene section or the promoter section on our website. This vector can also be used to force recombination into the genome of mammalian cells not expressing SV40 large T antigen following transfection and hygromycin selection although this method can be inefficient. If cells are expressing the SV40 large T antigen those that integrate the plasmid would likely experience chromosomal instability because the large T antigen would initiate DNA replication from within the host chromosome.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6771 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection hygromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-ILUMENA - MAMMALIAN SECRETED LUCIFERASE VECTOR OGS514-5UG
NACRES: NA.85 General descriptionThis plasmid contains the CMV promoter upstream of our standard multiple cloning site to allow the production of proteins in mammalian cells. The vector also contains a secreted luciferase reporter gene that is away from the multiple cloning site. The reporter is unique to our plasmids and exhibits high levels of luminescence in the supernantent of eukaryotic cells. The substrate for the luciferase is coelenterazine.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6183 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene iLumena luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-KRYFP - MAMMALIAN YFP REPORTER VECTOR OGS513-5UG
General descriptionA CMV expression plasmid that allows for the insertion of exogenous transgenes for expression in mammalian cells. The plasmid also contains a reporter gene cassette away from the multiple cloning site that allows for high levels of expression of Yellow Fluorescent protein. The reporter in this plasmid was original developed by DNA2. 0 and is called Kringle YFP.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6258 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene YFP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-NEO/G418 ASCI - G418 SELECTION PLASMID OGS22-5UG
NACRES: NA.85 General descriptionThis vector contains a Neomycin (Neo)/G418 resistance expression cassette under control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our vectors. The entire Ub Neo/G418 cassette is flanked by AscI sites. This vector can be used to force recombination into the genome of mammalian cells following transfection and selection using G418 although this method can be inefficient. It can also be used conjunction with the SV40 origin of replication in cells expressing the SV40 large T antigen (such as 293T cells) to make stable cells lines following transfection and selection.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4256 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection neomycin / G418 reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-NEO/G418-SV40 ORI SBFI - G418 / SV40 ORI PLASMID OGS138-5UG
NACRES: NA.85 General descriptionThis vector contains a Neomycin (Neo) resistance expression cassette under the Ub promoter. It also contains the Simian Virus 40 (SV40 origin of replication. This can be used to increase transgene signal following transient transfection. It is also reported that it is possible to make cell lines containing the plasmid as a multi-copy episome however we have not validated it for this purpose. The Ub Neo cassette is flanked by AscI sites in this plasmid and is not designed to be split up. If you would like the Neomycin gene alone or the Ub promoter alone please see the mammalian selection gene section or the promoter section on our website. This vector can also be used to force recombination into the genome of mammalian cells not expressing SV40 large T antigen following transfection and Neomycin selection although this method can be inefficient. If cells are expressing the SV40 large T antigen those that integrate the plasmid would likely experience chromosomal instability because the large T antigen would initiate DNA replication from within the host chromosome.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6532 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection neomycin / G418 reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-PURO ASCI - PUROMYCIN SELECTION PLASMID OGS21-5UG
NACRES: NA.85 General descriptionThis plasmid contains a Puromycin (Puro) resistance expression cassette under the transcriptional control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our other plasmids. The Ub Puro cassette is flanked by AscI sites but is not designed to be split up. If you would like the Puromycin gene alone or the Ub promoter alone please see the mammalian antibiotic selection gene section or the promoter section on our website. This plasmid can be used to force recombination into the genome of mammalian cells following transfection and selection although this method can be inefficient. It can also be used conjunction with the SV40 origin of replication in cells expressing the SV40 large T antigen (such as 293T cells) to make stable cell lines following transfection and selection.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6969 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-PURO ASCI-EMCV-FLUC - EMCV IRES LUCIFERASE PUROMYCIN SELECTION VECTOR OGS602-5UG
General descriptionThis vector contains the CMV promoter upstream of the internal ribosoms entry site (IRES) from Encephalomyocarditis virus (EMCV) which is driving the expression of the firefly luciferase reporter gene. The vector also contains a puromycin selection cassette that allows the production of stable cell lines in mammalian cells. Transgenes can be inserted upstream of teh EMCV IRES sequence using our standard multiple cloning site. This will allow both the gene of interest and the luciferase to be produced from the same mRNA molecule.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types. The IRES that is used to drive luciferase expression from the CMV promoter is considerably weaker than the CMV promoter and typically yields 15-20 fold lower protein levels.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 8368 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-PURO-SV40 ORI SBFI - PUROMYCIN / SV40 ORI PLASMID OGS136-5UG
NACRES: NA.85 General descriptionThis vector contains a puromycin (puro) resistance expression cassette under the Ub promoter. It also contains the Simian Virus 40 (SV40 origin of replication. This can be used to increase transgene signal following transient transfection. It is also reported that it is possible to make cell lines containing the plasmid as a multi-copy episome however we have not validated it for this purpose. The Ub puro cassette is flanked by AscI sites in this plasmid and is not designed to be split up. If you would like the Puromycin gene alone or the Ub promoter alone please see the mammalian selection gene section or the promoter section on our website. This vector can also be used to force recombination into the genome of mammalian cells not expressing SV40 large T antigen following transfection and puromycin selection although this method can be inefficient. If cells are expressing the SV40 large T antigen those that integrate the plasmid would likely experience chromosomal instability because the large T antigen would initiate DNA replication from within the host chromosome.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6342 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-RLUC ASCI - RENILLA LUCIFERASE PLASMID OGS156-5UG
NACRES: NA.85 General descriptionThis plasmid contains a Renilla reniliformis (Sea Pansy) luciferase expression cassette under the control of the Ubiquitin promoter that is designed as an add-on to any of our other plasmids. The Ub luciferase cassette is flanked by AscI sites but is not designed to be split up. If you would like the luciferase gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus promoter driven plasmid in the same format if you require an expression vector with lower levels of reporter gene activity.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6486 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-SEAP ASCI - ALKALINE PHOSPHATASE PLASMID OGS157-5UG
NACRES: NA.85 General descriptionThis plasmid contains a Secreted Alkaline Phosphatase (SEAP) expression cassette under the control of the Ub promoter that is designed to be used as an add-on to any of our other plasmids. The Ub expression cassette is flanked by AscI sites but is not designed to be split up. If you would like the SEAP gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus promoter driven plasmid in the same format if you require an expression vector with lower levels of reporter gene activity.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 7113 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene SEAP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-ZEO ASCI - ZEOCIN SELECTION PLASMID OGS23-5UG
NACRES: NA.85 General descriptionThis vector contains a Zeocin (Zeo) resistance expression cassette under control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our vectors. The entire Ub Zeo cassette is flanked by AscI site. This vector can be used to force recombination into the genome of mammalian cells following transfection and selection although this method can be inefficient. It can also be used conjunction with the SV40 origin of replication in cells expressing the SV40 large T antigen (such as 293T cells) to make stable cells lines following transfection and selection.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteLegal InformationZeocin is a registered trademark of Cayla SarlПараметрыform buffered aqueous solution mol wt size 6342 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection Zeocin® reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-UB-ZEO-SV40 ORI SBFI - ZEOCIN RESISTANT / SV40 ORIGIN PLASMID OGS137-5UG
General descriptionThis vector contains a zeocin (zeo) resistance expression cassette under the Ub promoter. It also contains the Simian Virus 40 (SV40 origin of replication. This can be used to increase transgene signal following transient transfection. It is also reported that it is possible to make cell lines containing the plasmid as a multi-copy episome however we have not validated it for this purpose. The Ub zeo cassette is flanked by AscI sites in this plasmid and is not designed to be split up. If you would like the zeocin gene alone or the Ub promoter alone please see the mammalian selection gene section or the promoter section on our website. This vector can also be used to force recombination into the genome of mammalian cells not expressing SV40 large T antigen following transfection and zeocin selection although this method can be inefficient. If cells are expressing the SV40 large T antigen those that integrate the plasmid would likely experience chromosomal instability because the large T antigen would initiate DNA replication from within the host chromosome.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteLegal InformationZeocin is a registered trademark of Cayla SarlПараметрыform buffered aqueous solution mol wt size 6117 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection Zeocin® reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-VSVG - VSV G EXPRESSION PLASMID OGS592-5UG
NACRES: NA.85 General descriptionIn this vector the Vesicular Stomatitis Virus G (VSVG) protein is under transcriptional regulation of the CMV promoter
PSF-CMV-VSVG - VSV G expression plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: PSF-CMV-VSVG - VSV G expression plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.Analysis NoteПараметрыform buffered aqueous solution mol wt size 5746 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV-ZEO - CMV PROMOTER ZEOCIN SELECTION VECTOR OGS587-5UG
NACRES: NA.85 General descriptionThe CMV promoter drive expression of Zeocin resistance in this resistance construct providing a simple means for drug-based selection of transfected mammalian cells.
Promoter Expression Level: This plasmid vector contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: PSF-CMV-ZEO - CMV PROMOTER ZEOCIN SELECTION VECTOR contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the multiple cloning site (MCS) to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteLegal InformationZeocin is a registered trademark of Cayla SarlПараметрыform buffered aqueous solution mol wt size 4585 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection Zeocin® reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMV/T7 - DUAL PROMOTER CMV T7 PLASMID OGS147-5UG
NACRES: NA.85 General descriptionA versatile expression vector for use in mammalian cells and those cells expressing the T7 bacteriophage polymerase. This vector also contains a Kanamycin resistance cassette for growth and maintenance in E. coli.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4274 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: T7
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMVE - CMV ENHANCER EXPRESSION PLASMID OGS378-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of a small multiple cloning site (SalI to BstBI) that allows the insertion of a promoter of choice. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus however this is not always the case and will vary depending on the promoter being used.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4294 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMVE-BETA-D96GAL - CMV ENHANCER BETA GAL PLASMID OGS366-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the beta galactosidase (beta gal) reporter gene. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus however this is not always the case and will vary depending on the promoter being used.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 7406 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene beta Gal shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMVE-FLUC - CMV ENHANCER LUCIFERASE PLASMID OGS363-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the Photinus pyralis (FLuc) luciferase reporter. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus however this is not always the case and will vary depending on the promoter being used.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5912 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMVE-RLUC - CMV ENHANCER LUCIFERASE PLASMID OGS364-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the Renilla reniformis (RLuc) luciferase reporter. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus however this is not always the case and will vary depending on the promoter being used.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5198 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-CMVE-SEAP - CMV ENHANCER SEAP PLASMID OGS365-5UG
NACRES: NA.85 General descriptionThis plasmid contains the major immediate early cytomegalovirus (CMV) enhancer region upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. The enhancer can be used to increase the strength of weaker promoters whilst often retaining any regulation that they already have such as tissue specificity or transcription factor activation in response to stimulus however this is not always the case and will vary depending on the promoter being used.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5825 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: enhancer
Promoter type: mammalianbacteria selection kanamycin reporter gene SEAP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-Core Basic Cloning Vector OGS1-5UG
NACRES: NA.85 General descriptionA versatile cloning plasmid backbone. All of our inserts are compatible with this plasmid.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteOther NotesThis plasmid contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.Параметрыform buffered aqueous solution Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection ampicillin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-EF1 ALPHA - ELONGATION FACTOR ALPHA PROMOTER PLASMID OGS43-5UG
NACRES: NA.85 General descriptionPSF-EF1 ALPHA - ELONGATION FACTOR ALPHA PROMOTER PLASMID contains the EF1 ± promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4872 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: EF1-alpha
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-EF1-PURO - ELONGATION FACTOR 1 ALPHA PROMOTER PUROMYCIN PLASMID OGS599-5UG
NACRES: NA.85 General descriptionHere the puromycin resistance gene is under transcription regulation of the human Elongation Factor 1 alpha promoter giving long term expression that can be useful for selection of transfected mammalian cells to make stable cell lines
Promoter Expression Level: Molecular cloning vector contains the human Elongation factor 1 promoter to drive protein expression. The EF1 alpha promoter contains a large intron that helps to maintain expression of the promoter in long term culture. Although it shows signficantly lower expression in most cell types compared to the CMV promoter by transient transfection the promoter can maintain activity for longer in many cell types when making stable cell lines.ApplicationCloning in a gene: PSF-EF1-PURO - ELONGATION FACTOR 1 ALPHA PROMOTER PUROMYCIN PLASMID contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5437 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: EF1-alpha
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-EF1-UB-NEO/G418 ASCI - EF1 ALPHA PROMOTER G418 SELECTION PLASMID OGS606-5UG
NACRES: NA.85 General descriptionThis plasmid vector contains the EF1alpha promoter upstream of the MCS and Neomycin/G418 resistance driven by the ubiquitin promoter
Promoter Expression Level: This plasmid contains the human Elongation factor 1 promoter to drive protein expression. The EF1 alpha promoter contains a large intron that helps to maintain expression of the promoter in long term culture. Although it shows signficantly lower expression in most cell types compared to the CMV promoter by transient transfection the promoter can maintain activity for longer in many cell types when making stable cell lines. The ubiquitin promoter driving the G418 resistance gene demonstrates similiar levels of expression to the EF1 alpha promoter in most cell types. The Ubiquitin promoter contains a large intron that helps to maintain promoter activity.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6969 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: EF1-alpha
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-GAL1-URA3 - GALACTOSE INDUCIBLE YEAST PLASMID OGS536-5UG
General descriptionThis vector contains a galactose inducible yeast promoter that allows for regulated and induced expression of a protein of interest in Saccharomyces cerevisiae yeast. The vector also contains a uracil metabolite selection gene that allows the plasmid to be maintained in cell that are deficient in this gene when the cells are grown in media without uracil.
Promoter Expression Level: This plasmid contains the yeast inducible galactose transport gene GAL1 promoter. It can be used to induce protien expression in Saccharomyces cerevisiae using galactose containing media.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 6807 bp Origin of replication 2Micron, pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: GAL1
Promoter activity: inducible
Promoter type: yeastbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C yeast selection uracil
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-H1 - SIRNA/RNAI EXPRESSION PLASMID OGS52-5UG
NACRES: NA.85 General descriptionThe expression of short hairpin RNA in eukaryotic cells. The H1 is transcribed by RNA polymerase III (RNA pol III) which terminates transcription at a string of thymidine residues typically a minimum of five residues in mammalian cells. Unlike most SnapFastTM vectors this plasmid contains an RNA pol III terminator downstream of the XhoI site although it is more common to insert a terminator sequence at the end of the sequence you insert into the MCS that encodes a shRNA.
Promoter Expression Level:ApplicationMultiple cloning site notes: This vector has a modified MCS compared to standard plasmids. The ATG start codon in the NcoI site has been removed as have the Shine-Dalgarno and KOZAK sequences as these are not required for RNA Pol3 expressed transcripts.
The terminator for RNA polymerase III has been positioned immediately downstream of the XhoI site. There is an additional string of Thymidine residues (the terminator sequence for RNA Pol III) at the end of the SV40 polyA region however using this site will produce a long transcript and has not been validated. We recommend inserting your hairpin DNA sequence between Hind3 and XhoI. The theoretical RNA position +1 is the first base of the NotI restriction site.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3801 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: H1
Promoter activity: siRNA
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-LACI - LACI PROMOTER BACTERIAL VECTOR OGS502-5UG
NACRES: NA.85 General descriptionThis vector is designed for the expression of proteins in E. coli. It contains the promoter from the Lac operon to control the expression of transgenes. In order to use this plasmid you will need an E. coli cell line that expresses the LacI repressor this is a common feature of most cloning and protein expression E. coli strains.
Promoter Expression Level: This plasmid contains the Lac operon promoter which is inducible using IPTG to regulate transgene expression.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3897 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterialbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-LENTI - LENTIVIRUS PUROMYCIN CMV PLASMID OGS269-5UG
NACRES: NA.85 General descriptionPSF-LENTI - lentivirus puromycin CMV plasmid contains a HIV1 based lentivirus expression vector that can be used for the stable inteagration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pCCL lentivirus backbone and contains a range of modifications to make cloning easier. The plasmid vector contains an expanded multiple cloning site and is compatible with most of the plasmids we provide in our product range which allows sections to compiled to create complex expression vectors. This plasmid contains the CMV promoter to drive the expression of a gene of interest followed by a multiple cloning site and the PGK promoter to drive puromycin resistance.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The murine phosphoglycerate kinase (PGK) promoter driving puromycin expression allows for long term stable expression in vitro and in vivo in most types.ApplicationCloning in a gene: PSF-LENTI - lentivirus puromycin CMV plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.Analysis NoteПараметрыform buffered aqueous solution mol wt size 33440 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage bacteria selection ampicillin reporter gene none shipped in ambient storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV - MINIMAL CMV EXPRESSION PLASMID OGS613-5UG
General descriptionMinimal CMV promoter expression plasmid that allows the insertion of transcription factor binding sites upstream of the minimal promtoer to create promoters with novel functions such as responsiveness to transciption factors or drugs. Your gene of interest can be inserted into the MCS under control of the new promoter.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3765 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-BLAST - BLASTICIDIN SELECTION MINIMAL CMV VECTOR OGS583-5UG
NACRES: NA.85 General descriptionHere the blasticidin resistance gene is under transcriptional regulation of the minimal CMV promoter giving only low level expression in most mammalian cells. Expression of the resistance gene can be regulated by inserting additional regulatory components upstream of the promoter using a specially-positioned MCS for example sites that respond to specific transcription factors or to externally-applied drugs such as doxycycline. In this way sophisticated constructs can be prepared that provide blasticidin resistance only under certain pre-defined conditions.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4159 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection blasticidin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-FLUC - MINIMAL CMV PROMOTER LUCIFERASE PLASMID OGS570-5UG
NACRES: NA.85 General descriptionThe expression vector contains the minimal CMV promoter driving expression of Firefly luciferase. The vector normally mediates low levels of expression in most mammalian cells however there is an MCS immediately upstream of the minimal promoter that allows additional regulatory components to be inserted. These can include cell- or tissue-selective enhancers or repressors such as transcription factor binding sites or can be made responsive to external stimuli such as drugs. In this way a range of tuneable reporter systems can be prepared.
Promoter Expression Level: This cloning vector contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: PSF-MINCMV-FLUC - MINIMAL CMV PROMOTER LUCIFERASE PLASMID contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5383 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene firefly luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-FRCFP - MINIMAL CMV CFP CLONING PLASMID OGS575-5UG
NACRES: NA.85 General descriptionThe Frosty Cyan Fluorescence Protein (FrCFP) is under regulatory control of the minimal CMV promoter in this plasmid giving low level expression of the FrCFP transgene in most types of mammalian cells. Positive or negative regulatory elements can be introduced upstream of the minimal promtoer using a specially-positioned MCS to endow your required responsiveness. For example transcription factor binding sites could be introduced to provide tissue-selective transgene expression. Similarly drug-responsive sites could be introduced to provide expression regulated by externally-applied drugs.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4441 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene CFP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-HYGRO - HYGROMYCIN MINIMAL CMV PLASMID OGS580-5UG
NACRES: NA.85 General descriptionIn this plasmid the hygromycin resistance gene is regulated by the minimal CMV promoter giving low level expression in most mammalian cells. An MCS upstream of the promoter enables simple introduction of additional regulatory elements to provide responsiveness (for example) to cell-associated transcription factors or to externally-applied stimuli such as doxycycline. In this way a range of sophisticated constructs can be prepared that provide hygromycin resistance only under specific conditions.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4762 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-ILUMENA - SECRETED LUCIFERASE MINIMAL CMV PLASMID OGS578-5UG
NACRES: NA.85 General descriptionHere the iLumena (secreted) luciferase gene is under transcriptional regulation of the minimal CMV promoter giving low level expression of secreted luciferase in most types of mammalian cells. Levels of the reporter protein activity can be measured without lysing the cells. The activity of the minimal CMV promoter can be tuned using an upstream MCS to introduce sites that respond to cell-associated transcription factors (either stimulatory or repressive) or to exogenously-applied drugs. In this way a diverse range of promoter systems can be prepared using this plasmid.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4366 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene iLumena luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-KRYFP - MINIMAL CMV YFP EXPRESSION VECTOR OGS574-5UG
NACRES: NA.85 General descriptionIn this plasmid Kringle Yellow Fluorescence Protein (YFP) is under transcriptional control of the minimal CMV promoter giving low level expression in many mammalian cells. An MCS upstream of the minimal promoter can be used to introduce regulatory elements such as transcription factor-binding sites to endow tissue-selective expression or responsiveness to exogenously-applied drugs. This permits a range of sophisticated reporter systems to be prepared using this vector.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4441 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene YFP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-NEO/G418 - G418 SELECTION MINIMAL CMV VECTOR OGS581-5UG
General descriptionHere the neomycin/G418 resistance gene is under transcriptional control of the minimal CMV promoter giving basal levels of expression in most mammalian cells. An upstream MCS is positioned to allow inclusion of additional regulatory elements upstream of the minimal promoter allowing high levels of resistance to be conditional upon binding of specific transcription factors or on the presence of externally-applied drugs such as doxycycline. In this way the plasmid allows production of a range of sophisticated constructs providing G418/neomycin resistance only under specified conditions.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Quick-reference Plasmid Map,
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4525 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection neomycin / G418 reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-PURO - MINIMAL CMV PUROMYCIN VECTOR OGS579-5UG
NACRES: NA.85 General descriptionIn this plasmid the minimal CMV promoter regulates expression of the puromycin resistance gene providing basal levels of expression in most mammalian cells. An upstream MCS enables additional regulatory elements to be introduced upstream of the minimal promoter to make it responsive to cell-associated transcription factors (either stimulatory or repressive) or externally-applied drugs. In this way a range of sophisticated vectors can be prepared that provide puromycin resistance under carefully-defined conditions.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4330 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection puromycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-RLUC - MINIMAL CMV PROMOTER RENILLA PLASMID OGS571-5UG
General descriptionIn this plasmid the Renilla luciferase gene is under transcriptional regulation of the minimal CMV promoter. This system gives only low level expression in most mammalian cells however an MCS upstream of the minimal promoter can be used to insert positive- or negative- regulatory elements for example to introduce responsiveness to cell-associated transcription factors or exogenously-added drugs. In this way a range of reporter systems can be prepared.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 4669 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene Renilla luciferase shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-SEAP - ALKALINE PHOSPHATASE MINIMAL CMV PLASMID OGS577-5UG
NACRES: NA.85 General descriptionThis plasmid has the minimal CMV promoter regulating expression of secreted alkaline phosphatase (SEAP) allowing low levels of expression in most mammalian cells. The activity of the minimal promoter can be tuned using a specially-positioned upstream MCS to introduce sites that respond to externally-applied drugs or cell selective (stimulatory or repressive) transcription factors providing a diverse range of possible reporter systems. Transgene expression can be measured without cell lysis as the SEAP is secreted from the transfected cell.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 5296 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene SEAP shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID OGS582-5UG
NACRES: NA.85 General descriptionPSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID has the Zeocin resistance gene under transcriptional control of the minimal CMV promoter giving basal expression in most mammalian cells. Expression levels can be influenced by inserting additional regulatory elements upstream of the minimal promoter using a specially-positioned MCS for example to provide dependence on cell-associated transcription factors or externally applied drugs such as doxycycline. In this way a range of sophisticated constructs can be prepared that endow Zeocin resistance only under specific conditions.
Promoter Expression Level: This plasmid vector contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.ApplicationCloning in a gene: PSF-MINCMV-ZEO - MINIMAL CMV ZEOCIN RESISTANT PLASMID contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
Sequence
Quick-reference Plasmid Map,
Analysis NoteLegal InformationZeocin is a registered trademark of Cayla SarlПараметрыform buffered aqueous solution mol wt size 4105 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin mammalian cells selection Zeocin® reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PSF-MINPROM - MINIMAL PROMOTER EXPRESSION PLASMID OGS377-5UG
General descriptionThis plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of a gene of interest. The promoter multiple cloning site extends from the SalI restriction site to the BstBI restriction site. Downstream sites have other functions in our plasmid system for example adding n-terminal peptide tags.
Promoter Expression Level:ApplicationCloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Sequence
Analysis NoteПараметрыform buffered aqueous solution mol wt size 3885 bp Origin of replication pUC (500 copies) Peptide cleavage no cleavage Promoter Promoter name: HSV-TK
Promoter activity: minimal promoter
Promoter type: mammalianbacteria selection kanamycin reporter gene none shipped in ambient storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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