Molecular Biology &amp; Functional Genomics

3.4.24.3 ( BRENDA | IUBMB )

Collagenase D is prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.

Collagenase from C. histolyticum is widely used for the disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors) and for the preparation of single cell suspensions for the establishment of primary cell culture systems. Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.
Clostridium collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc. However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically.

Collagenase from Roche is assayed in Wnsch units (1 µmol of product formed per minute at +25 °C with Wnsch substrate).
Frequently, collagenase activities are given in Mandl units (1 µmol leucine liberated from collagen in 5 hours at +37 °C).
Unfortunately, there is no consistent conversion factor between the two units of activity, since the Mandl unit depends, in part, on the concentration of contaminating proteases in the collagenase preparation, an indefinable variable. A purer collagenase preparation would actually give a lower specific activity in Mandl units than a crude preparation. Clostridium preparations typically give conversion factors of approximately 1:1800 (e.g., a particular lot of Clostridium collagenase contained approximately 0.15 Wnsch U/mg and 250 Mandl U/mg).

Contents
Lyophilizate, nonsterile

Unit Definition

Lyophilizate, nonsterile.

Activator: Ca2+
Working concentration: 0.5 - 2.5mg/ml
Storage conditions (working solution): -15 to -25°C
Roche recommends reconstituting only the amount of lyophilizate needed for immediate use. The reconstituted solution can be stored at -15 to -25°C for up to one week. Avoid repeated freezing and thawing since activity decreases after reconstitution.

Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Enzyme activity:
Collagenase activity: >0.15 U/mg (according to Wunsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase D has a normal to high collagenase activity and very low tryptic activity (usually <0.2 units/mg, BAEE as substrate).

Reconstitution in any balanced salt solution (e.g., HBSS)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
sterility	non-sterile
form	lyophilized
packaging	pkg of 100 mg (11088858001), pkg of 2.5 g (11088882001), pkg of 500 mg (11088866001)
Торговая марка	Roche
concentration	(working concentration: 0.5 - 2.5 mg/ml)
optimum pH	6.0-8.0
shipped in	wet ice
storage temp.	2-8°C

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Collagenase H

3.4.24.3 ( BRENDA | IUBMB )

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.
Specificity
Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.
Physical form
Lyophilizate, nonsterile
Preparation Note
Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wunsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca2+
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.
Reconstitution
Reconstitution in any balanced salt solution (e.g., HBSS)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	non-sterile
form	lyophilized
packaging	pkg of 100 mg (11074032001), pkg of 2.5 g (11087789001), pkg of 500 mg (11074059001)
Торговая марка	Roche
optimum pH	6.0-8.0
shipped in	wet ice
storage temp.	2-8°C

The most common technique in affinity purification of proteins involves engineering a sequence of 6 to 14 histidines into the N- or C-terminal (or even on an exposed loop) of the protein. Such polyhistidine stretches bind strongly to divalent metal ions such as nickel and cobalt. This effect can be used to separate proteins. Metal ions can be immobilized on a matrix using a chelator, which still allows the ion to interfere with the polyhistidine tag of the protein. When these his-tagged proteins are passed through a column containing immobilized metal ions, the proteins bind via the tag to the column. Nearly all untagged proteins pass through the column. The protein is released from the column by elution with either imidazole, which competes with the polyhistidine tag for binding to the column, or by a decrease in pH, which decreases the affinity of the tag for the resin. While this procedure is generally used for the purification of recombinant proteins with an engineered affinity tag, it can also be used for natural proteins with an inherent affinity for divalent cations.
Features and Benefits
cOmplete His-Tag Purification Columns are available in 2 sizes and prepacked with cOmplete His-Tag Purification Resin. The cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purifications of His-tagged proteins from total lysates. Roches propriety nickel-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The wide choice of compatible ingredients allows optimization of buffers for maximum protein stability and solubility.

cOmplete His-Tag Purification Columns are fully compatible with standard purification systems such as KTA Systems (Cytiva).

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cOmplete™ His-Tag Purification Column

Use the buffer conditions best suited to your protein

Keep your protein comfortable and let it, not your purification resin, determine whether you use DTT, EDTA, or other buffer substances.

Repeatedly obtain highly pure protein

Single step purification without resin recharging.

Protect your protein from toxic nickel

Reduce protein oxidation and aggregation caused by resins that leach nickel.

Work in a safe and eco-friendly environment

Avoid handling of toxic nickel and completely eliminate disposal costs.
Packaging

06781535001 (5 mL column; 1 column containing 5 ml of cOmplete His-Tag Purification Resin)
06781543001 (5 x 1 mL columns; 5 columns containing 1 ml of cOmplete His-Tag Purification Resin)

Compatibility
Compatibility for long term storage: 20% ethanol, pH 4.0 to pH 9.0
Compatibility during chromatography: The resin is compatible with 10mM EDTA, 10mM DTT during the purification (1 hour incubation), 6M guanidinium-HCl, 8M urea, pH 2.0 to pH 14.0.
Compatibility during cleaning: 4% SDS Form cOmplete His-Tag Purification Resin filled in columns, pre-charged with Ni2+ stored in 20% ethanol.

packaging	pkg of 5 x 1 mL (columns (06781543001)), pkg of 5 mL (column (06781535001))
Торговая марка	Roche
parameter	1,420 cm/hr max. flow rate
capacity	40 mg/mL, resin binding capacity
shipped in	wet ice
storage temp.	2-8°C

Bead size: 45 to 165µM
Binding capacity: 40mg protein per ml bed volume of resin. The binding capacity of the resin to various types of proteins may vary according to the protein characteristics such as the size of the protein. cOmplete His-Tag Purification Columns bind with a high specificity to the polyhistidine-tagged protein. As a consequence, the binding kinetics may appear to be different when compared to conventional metal chelate matrices. Full capacity of cOmplete His-Tag Purification Columns can be achieved by allowing more time for the protein to bind to the resin by lowering the flow rate during the chromatography purification procedure.
Maximal linear flow rate: 1,420cm/hour
Recommended volumetric flow rate: The volumetric flow rate is a function of the cross section of the column. Using the following formula, a linear flow rate can be converted to a volumetric flow (mL/min.): Linear flow rate (cm/hour) x column cross sectional area (cm2)/60. The column cross sectional area is defined as x r2, whereas is the constant pi and r is the inner radius of the column.
Recommended imidazole concentration for load/wash: Nonspecific binding of proteins without a His-tag is low. Use up to 5mM imidazole in load and/or wash buffers. If establishing a new assay for purification of His-tag proteins with cOmplete His-Tag Purification Columns, do not use imidazole. If, e.g., the purity of the His-tag protein needs to be improved following this first step, use imidazole in a final concentration of up to 5mM in a second step.
Recommended imidazole concentration for elution: Up to 500 mM. Please note: Contrary to other available resins, bound His-tagged protein typically elutes from cOmplete His-Tag Purification Columns with a lower imidazole concentration, e.g., 25 to 45mM.
Compatibility for long term storage: 20% ethanol, pH 4.0 to pH 9.0
Compatibility during chromatography: The resin is compatible with 10mM EDTA, 10mM DTT during the purification (1 hour incubation), 6M guanidinium-HCl, 8M urea, pH 2.0 to pH 14.0.
Compatibility during cleaning: 4% SDS
The cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for the purification of histidine-tagged proteins via batch or liquid chromatography procedures from total lysates. In contrast to all currently available resins, which are primarily based on NTA or IDA chelator chemistry, this resin is the only resin on the market which tolerates buffers that contain EDTA. Because EDTA is a known inhibitor of metalloproteases–which are frequently present in any cell type–this chromatography material provides the option to increase the protection of your target protein by supplementing your buffers with EDTA. This resin therefore allows increased protection of your proteins against degradation and, at the same time, enriches the protein using the well-established affinity purification method.
In addition, the resin maintains its binding capacity whether you are using low- or high-molecular weight proteins, and its specificity allows a one-step purification. By using one of the strongest chelators available, minimal contamination of your flow-through fractions with metal ions is ensured, safeguarding your downstream applications.
The cOmplete His-Tag Purification Resin is also available as prepacked format: with 1mL or 5mL resin.

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cOmplete™ His-Tag Purification Resin

Recombinant Protein Expression

Purifying a protein of interest is often essential for determining its function, structure, or interactions, for raising specific antibodies, or preparing enzymes for practical applications. Isolation of naturally expressed proteins from their original source can be a complex process involving numerous chromatographic steps. Recombinant protein expression in dedicated host organisms can greatly simplify this task. Such expression systems generally ensure higher expression levels. Fusing the target protein to a tag also confers advantageous binding ability to an affinity matrix.
Protein Purification using Immobilized Ni2+
The most common technique in affinity purification of proteins involves engineering a sequence of 6 to 14 histidines into the N- or C-terminal (or even on an exposed loop) of the protein. Such polyhistidine stretches bind strongly to divalent metal ions such as nickel and cobalt. This effect can be used to separate proteins. Metal ions can be immobilized on a matrix using a chelator, which still allows the ion to interfere with the polyhistidine tag of the protein. When these his-tagged proteins are passed through a column containing immobilized metal ions, the proteins bind via the tag to the column. Nearly all untagged proteins pass through the column. The protein is released from the column by elution with either imidazole, which competes with the polyhistidine tag for binding to the column, or by a decrease in pH, which decreases the affinity of the tag for the resin. While this procedure is generally used for the purification of recombinant proteins with an engineered affinity tag, it can also be used for natural proteins with an inherent affinity for divalent cations.
His-Tags

Ideally, the His-tagged target protein binds much stronger to the Ni2+ chelate matrix than endogenous histidine-containing protein of the expression host. Relative binding strength depends on how many histidines can bind simultaneously to the matrix (avidity effect). Longer His-tags confer stronger binding and better separation of the target from potentially contaminating host proteins. The classic His-tag has six consecutive histidines. Tags with 10 to 14 histidines may produce a better purification. Most importantly, His-tagged proteins can be purified using Ni2+ chelate matrices under both native and denaturing conditions. Due to their hydrophilic and flexible nature, these matrices increase the solubility of the target proteins and only rarely interfere with protein function. This unique combination of features enables the His-tag to be a versatile tool for a wide range of protein purification applications.
Features and Benefits
cOmplete His-Tag Purification Resin is the only resin for purifying large amounts of protein without compromises.

Use the buffer conditions best suited to your protein: Keep your protein comfortable and let it, not your purification resin, determine whether you use DTT, EDTA, or other buffer substances.
Repeatedly obtain highly pure protein: Single step purification without resin recharging.
Protect your protein from toxic nickel: Reduce protein oxidation and aggregation caused by resins that leach nickel.
Work in a safe and eco-friendly environment: Avoid handling of toxic nickel and completely eliminate disposal costs.

Physical form
50% resin in suspension, pre-charged with Ni2+

For life science research only. Not for use in diagnostic procedures.
EDTA-compatible resin for the purification of poly-histidine-tagged proteins.

packaging	pkg of 200 mL (05893801001 settled resin volume), pkg of 25 mL (05893682001 settled resin volume)
mfr. no.	Roche
matrix	Sepharose-CL 6B
shipped in	ambient
storage temp.	2-8°C

cOmplete™ Lysis-M 4719956001

The cOmplete™ Lysis-M mammalian cell protein extraction reagent contains a mild ready-to-use detergent (in 25mM bicine buffer, pH 7.6) that efficiently lyses mammalian cells and eliminates the need for scraping, sonication, or freeze-thaw cycles. It is required for the gentle extraction of proteins from both the cytoplasm and the nucleus of cultured mammalian cells. Protein yields obtained with this kit are 20 to 25% higher compared to three cycles of freeze-thaw, and approximately 20% higher than 2 minutes of sonication (with 50% pulse). Extracted proteins can be further purified or analyzed in downstream applications. Efficient lysis of mammalian cells occurs in only 5 minutes at room temperature.
The ready-to-use cOmplete™ Mini Protease Inhibitor Cocktail Tablets, included in the kit, function optimally with the kits Lysis-M reagent. The cOmplete™ Mini Protease Inhibitor Tablets allow the inhibition of a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. cOmplete™ Lysis-M reagent helps in protein extraction and simultaneously inhibit protease activity during the initial extraction steps. cOmplete™ Mini Protease Inhibitor Tablets contain both irreversible and reversible protease inhibitors.

Lysis-M reagent is compatible with many different applications:

reporter assays (e.g., -galactosidase, luciferase, chloramphenicol acetyltransferase)
immunoassays (e.g., Western blots, ELISAs, RIAs)
protein assays (e.g., protein kinase A, protein kinase C, and tyrosine kinase)
protein purification

Furthermore, the cell lysate is compatible with protein assays such as Coomassie staining and BCA (2-Benzoyloxycinnamaldehyde) protein assays, and the reagent can be removed by dialysis.
Note: cOmplete™ Mini tablets (included in the kit) contain EDTA (ethylenediaminetetraacetic acid). If the protein of interest is to be purified by IMAC (immobilized metal-chelate affinity chromatography), e.g., Poly-His tagged recombinant proteins, EDTA has to be removed (e.g., by dialysis) prior to the chromatography. Alternatively, the cOmplete™ Lysis-M EDTA-free product can be used.
Features and Benefits

Preserves protein structure, since reagents will not denature or otherwise alter proteins.
Saves money, since one product protects against many proteases.
Saves steps, since extracted proteins may be used directly in many downstream procedures.

Packaging
1 kit containing 2 components. Each tablet is sufficient for 10 mL of extract. For the lysis of up to 100 g of mammalian cells.
Preparation Note
Intended use: Simultaneous extraction and protection of soluble proteins from mammalian cells.
Sample material: Mammalian cells in culture (e.g., Cos-7 cells, harvested at confluency).
Time required: 5 minutes (cell lysis at room temperature).
Number of extractions: 20 (10 ml each).
Effective against: Serine proteases, cysteine proteases, metalloproteases.
Note: Each cOmplete, Mini tablet is concentrated enough to inhibit proteases in 10 ml of extract.

For life science research only. Not for use in diagnostic procedures.

Торговая марка	Roche
shipped in	ambient
storage temp.	room temp

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™ Lysis-M EDTA-free 4719964001

The cOmplete® Lysis-M EDTA-free mammalian cell protein extraction reagent contains a mild detergent (in 25mM bicine buffer, pH 7.6). cOmplete Lysis-M EDTA-free is intended for the efficient and gentle extraction of proteins from both the cytoplasm and the nucleus of cultured mammalian cells. Efficient lysis of mammalian cells occurs in only 5 minutes at room temperature, eliminating the need for scraping, sonication, or freeze-thaw cycles.
The ready-to-use cOmplete Mini EDTA-free Protease Inhibitor Cocktail Tablets, included in the kit, work optimally with the kit s Lysis-M reagent. cOmplete Lysis-M EDTA-free provides a very convenient and efficient way to extract proteins and simultaneously inhibit protease activity during the initial extraction steps. cOmplete Mini EDTA-free Protease Inhibitor Cocktail Tablets contains both irreversible and reversible protease inhibitors. cOmplete Mini EDTA-free tablets efficiently inhibit a wide range of serine and cysteine proteases, but not metalloproteases.
The extracted proteins can be further purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed in downstream applications.

Lysis-M reagent is compatible with many different applications, including reporter assays (e.g., -galactosidase, luciferase, chloramphenicol acetyltransferase), immunoassays (e.g., Western blots, ELISAs, RIAs), protein assays (e.g., protein kinase A, protein kinase C, and tyrosine kinase), and protein purification. Furthermore, the cell lysate is compatible with protein assays such as Coomassie staining and BCA (2-benzoyloxycinnamaldehyde) protein assays, and the reagent can be removed by dialysis.
Note: cOmplete Mini EDTA-free tablets (included in the kit) are employed to stabilize those extracts where the activity of metal-containing proteins must not be affected. Since EDTA interferes with IMAC (immobilized metal-chelate affinity chromatography), cOmplete Mini EDTA-free is preferentially used in the isolation process of Poly-His tagged fusion proteins or subsequent assays.
Features and Benefits

Compltely and gently lyses mammalian cells in just 5 minutes.
Saves time by eliminating scraping, sonication, and freeze-thaw cycles.
Produce higher yields of extracted protein than sonication.
Preserves protein structure, since reagents will not denature or otherwise alter proteins.
Saves money, since one product protects against many proteases.
Saves steps, since extracted proteins may be used directly in many downstream procedures (even those that would be affected by EDTA, such as IMAC).
Intended use: Simultaneous extraction and protection of soluble proteins from mammalian cells.
Sample material: Mammalian cells in culture (e.g., Cos-7 cells, harvested at confluency).
Time required: 5 minutes (cell lysis at room temperature).
Number of extractions: 20 (10 ml each).
Effective against: Serine proteases, cysteine proteases (but not metalloproteases).

Packaging
1 kit containing 2 components. Each tablet is sufficient for 10 mL of extract. For the lysis of up to 100 g of mammalian cells.
Storage and Stability
Store cOmplete Protease Inhibitor Tablets at 2–8 °C, Lysis-M-reagent at 15–25 °C.

For life science research only. Not for use in diagnostic procedures.

Roche is a registered trademark of Roche Molecular Systems, Inc.
cOmplete is a trademark of Roche

Торговая марка	Roche
shipped in	ambient

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.

cOmplete™ Protease Inhibitor Cocktail

cOmplete™ Protease Inhibitor Cocktail has been used as a component of

lysis buffer for the homogenisation of rat cavernosal smooth muscle (CSM) for obtaining membranous and cytosolic fractions
cold buffer A for preparing cytosolic and nuclear extracts
lysis buffer for homogenisation of tissues for determining protein expression by western blot
phosphate buffered saline (PBS) for the lysis of rat retinae for enzyme linked immunosorbent assay (ELISA)

cOmplete™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete contains EDTA (1 tablet yields a 1 mM EDTA solution in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+ , Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete EDTA-free can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is present.
cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet is identical to the normal cOmplete Tablet.

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Working concentration: 1 tablet per 50 ml extraction solution
Working solution: Preparation of Working Solution:

One cOmplete Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.
Preparation of Stock solution (25 X conc.):

Dissolve one cOmplete Protease Inhibitor tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0. The stock solution can also be prepared in 10 mM Tris buffer, pH 7.2 to 7.5.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can also be used in thiol-containing solutions at 15 to 25 °C.

Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate. For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	vial of 20 tablets (11697498001), vial of 3 x 20 tablets (11836145001)
Торговая марка	Roche
optimum pH	<7.5
solubility	aqueous buffer: soluble
absorption	0.08 at 280 nm
shipped in	wet ice
storage temp.	2-8°C

cOmplete™ Protease Inhibitor Cocktail 4693116001

cOmplete Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete tablets contain EDTA and both irreversible and reversible protease inhibitors. Protease inhibitors present in cOmplete Tablets do not form irreversible complexes with the SH groups of proteins.
Specificity
Inhibits a broad spectrum of serine proteases, cysteine proteases, and metalloproteases, as well as calpains.

cOmplete™ Protease Inhibitor Cocktail has been used as an component in

extraction buffer for protein extraction from Arabidopsis seedling
in radioimmunoprecipitation assay (RIPA) buffer for the isolation of protein from lymphocytes
the extraction buffer for the lysis of protoplast.

cOmplete™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete contains EDTA (1 tablet yields a 1 mM EDTA solution in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography. Alternatively, the product cOmplete, EDTA-free, can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is present.
cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet, is identical to the normal cOmplete Tablet.
Features and Benefits
Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

Packaging
20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in foil blister packs
Preparation Note
Working concentration: 1 tablet per 50ml extraction solution
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not finger nail) to prevent the breakage of tablets.
Preparation of Working Solution
One cOmplete tablet is sufficient for the inhibition of the proteolytic activity in 50ml extraction solution. When a very high proteolytic activity is present, one tablet should be used for 25ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.
Preparation of Stock solution (25 X conc.)
Dissolve one cOmplete tablet in 2ml dist. water or in 2ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8°C for 1 to 2 weeks, or at least 12 weeks at -15 to -25°C.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.
Storage and Stability
Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pkg of 20 tablets
Торговая марка	Roche
solubility	water: soluble
absorption	0.08 at 280 nm
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H314
pcodes	P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete ULTRA Tablets contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. As this tablet contains EDTA, metalloproteases are also inhibited. One cOmplete ULTRA Tablet is sufficient for a volume of 50 ml extraction solution. cOmplete ULTRA Tablets contain both irreversible and reversible protease inhibitors. Tablets are supplied in glass vials. Alternatively, these tablets are also available in EASYpack blister packs. EASYpacks are the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer.
Specificity
Inhibits a broad spectrum of serine proteases, cysteine proteases, aspartic proteases, and metalloproteases.

cOmplete™ ULTRA Tablets, glass vials Protease Inhibitor Cocktail

During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended variety of proteases. This new formulation shows high inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for improved efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
cOmplete™ ULTRA Tablets, glass vials Protease Inhibitor Cocktail has been used as a component of lysis buffer for the extraction of recombinant proteins from Escherichia coli.
Features and Benefits

Protect your precious proteins.

Choose cOmplete ULTRA for even more efficient protease inhibition in eukaryotic, yeast, and bacterial cell lysates.

Why settle for less.

Benefit from 8 powerful protease inhibitors including EDTA – more than in other inhibitor tablets.

Defend against a broad range of proteases.

Reliably inhibit serine, cysteine, metallo-, and aspartic proteases.

Convenient and safe tablet format.

Eliminate weighing small amounts of individual inhibitors which might be hazardous. Simply drop a tablet into your buffers.

Suitable for IMAC purification

Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in a glass vial. Each tablet is sufficient for a volume of 50 ml extraction solution.
Preparation Note
Working concentration: 1 Tablet per 50 ml extraction solution.
1 Tablet contains 18.5 mg EDTA, yielding a 1 mM solution of EDTA in the 50 ml solution.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet to 25 ml double-distilled water in a PE or PP tube.
• Vortex 4 min, until the tablet is dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.

For life science research only. Not for use in diagnostic procedures.

form	tablet
usage	sufficient for 50 mL (05892988001), sufficient for 50 mL (06538304001)
packaging	pkg of 2 x 10 tablets (05892988001), pkg of 6 x 10 tablets (06538304001)
Торговая марка	Roche
solubility	aqueous buffer: soluble
absorption	0.4 at 280 nm (1x solution)
shipped in	ambient
storage temp.	2-8°C

cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail 5892970001

cOmplete ULTRA Tablets contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. As this tablet contains EDTA, metalloproteases are also inhibited. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA, Mini Tablet is sufficient for a volume of 10 ml extraction solution.
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. Metalloproteases, which are inhibited by EDTA, are fairly prevalent in, for example, bacteria.
You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
Specificity
Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases completely when divalent cations like Ca2+, Mg2+, or Mn2+ are present

cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay buffer (RIPA buffer). It has also been used to prevent protein degradation and coagulation in blood samples.
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).
Features and Benefits

Ultra efficient protection

The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.

Convenient tablet format

Eliminate weighing small amounts, and instantly protect your proteins against an exceptionally wide range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.

Suitable for IMAC purification

Purify poly-histidine tagged proteins.

Ensure laboratory safety

Avoid contact with hazardous compounds using cOmplete ULTRA Tablets.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add cOmplete ULTRA Tablet to 5 ml double-distilled water in a PE or PP tube.
• Vortex 3 min or until the tablet is completely dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• Stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media.
For the stock solution, use water only.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pkg of 30 tablets (individually packed in foil blister packs)
Торговая марка	Roche
solubility	water: soluble
absorption	0.4 at 280 nm (1x solution)
shipped in	ambient
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H314 - H412
pcodes	P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™ ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail 5892791001

Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. However, metalloproteases are prevalent in (for example) bacteria and some techniques require that EDTA be omitted.
cOmplete ULTRA Tablets, Mini, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA Tablet, Mini, EDTA-free is sufficient for a volume of 10 ml extraction solution.
cOmplete ULTRA Tablets, Mini, EDTA-free are identical to cOmplete ULTRA Tablets, Mini, the only difference being that no EDTA or other chelating agents (e.g. EGTA) are included.
Specificity
Inhibits serine proteases, cysteine proteases, and aspartic proteases. Does not inhibit metalloproteases.

During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete™ ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
This tablet does not contain EDTA; therefore, metalloproteases are not inhibited.
For the purification of histidine-tagged proteins using Immobilized Metal Ion Affinity Chromatography (IMAC), this EDTA-free tablet is recommended for applications using non-EDTA compatible IMAC resins. Alternatively, an EDTA-containing cOmplete ULTRA, Mini tablet may be used in combination with Roche s new EDTA-compatible chromatography material, cOmplete His-Tag Purification Resin.
cOmplete™ ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail has been used as a component of

homogenization buffer for muscle samples for immunoblotting sample preparation
radioimmunoprecipitation assay buffer (RIPA buffer) for protein extraction from brain samples for western blotting
lysis buffer for the human embryonic kidney 293 (HEK293) lysate preparation for western blotting

Choose cOmplete ULTRA for even more efficient protease inhibition in eukaryotic, yeast, and bacterial cell lysates.

Protect your precious proteins.

Why settle for less.

Benefit from 7 powerful protease inhibitors – more than in other EDTA-free inhibitor tablets.

Defend against a broad range of proteases.

Reliably inhibit serine, cysteine, and aspartic proteases.

Convenient and safe tablet format.

Eliminate weighing small amounts of individual inhibitors which might be hazardous. Simply drop a tablet from the blister into your buffers.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add one cOmplete ULTRA Tablet, Mini, EDTA-free to 5 ml double-distilled water in a PE or PP tube.
• Vortex 3 min or until the tablet is completely dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pkg of 30 tablets (individually packed in foil blister packs)
Торговая марка	Roche
solubility	water: soluble
absorption	0.4 at 280 nm (1x solution)
shipped in	ambient
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H314 - H412
pcodes	P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete EDTA-free Tablets are identical to cOmplete Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

cOmplete™, EDTA-free Protease Inhibitor Cocktail

Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.

cOmplete™, EDTA-free is used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10 ml), we recommend to use cOmplete, Mini, EDTA-free.
cOmplete™, EDTA-free Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay (RIPA) lysis buffer for resuspending Chinese hamster ovary (CHO) cells. It has also been used as a component of resuspension buffer for HCT116 colorectal cancer cells.
cOmplete™, EDTA-free Protease Inhibitor Cocktail has been used as as a component of

immunoprecipitation lysis buffer (IPLS) for lysis of human embryonic kidney cells 293 (HEK293T cells) for glutathione co-precipitation
radioimmunoprecipitation assay (RIPA) buffer for lysis of cells for western blotting
buffer A for the lysis of bacterial cells

Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Working concentration: 1 tablet per 50 ml extraction solution
Working solution: Preparation of Working Solution

One cOmplete EDTA-free Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 50 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.
Preparation of Stock solution (25 X conc.)

Dissolve one cOmplete EDTA-free tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	vial of 20 tablets (11873580001), vial of 3 x 20 tablets (05056489001)
Торговая марка	Roche
solubility	aqueous buffer: soluble
absorption	0.08 at 280 nm
shipped in	wet ice
storage temp.	2-8°C

cOmplete™, EDTA-free Protease Inhibitor Cocktail 4693132001

cOmplete, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA - no other chelating agents, e.g. EGTA as well - leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete EDTA-free Tablets are identical to cOmplete Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.

cOmplete™, EDTA-free Protease Inhibitor Cocktail has been used as a component in

lysis buffer for the lysis of transfected human embryonic kidney (HEK293T) cells and in radioimmunoprecipitation assay (RIPA) lysis buffer for the homogenization of zebrafish embryo
tissue protein extraction reagent (T-PER) for the homogenization of hippocampus tissue
sodium dodecyl sulphate (SDS) lysis buffer for the lysis of hepatocytes

cOmplete™, EDTA-free, efficiently inhibits serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts. Metallo- and aspartic proteases are not inhibited.
cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10ml), we recommend to use cOmplete, Mini, EDTA-free.
One cOmplete, EDTA-free tablet was added per 50 ml incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete, EDTA-free tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25°C.

20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.

Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

Packaging

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Tablets in foil blister packs

Working concentration: 1 tablet per 50 ml extraction solution
Working solution: Handling

Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution

One cOmplete EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 50 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.
Preparation of Stock solution (25 X conc.)

Dissolve one cOmplete EDTA-free tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; an be used in thiol-containing solutions at 15 to 25 °C.

Storage and Stability

Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pkg of 20 tablets (individually packed in foil blister packs)
mfr. no.	Roche
solubility	aqueous buffer: soluble, water: soluble
absorption	0.08 at 280 nm
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H314
pcodes	P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™, Mini Protease Inhibitor Cocktail 11836153001

cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains.
Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete, Mini Protease Inhibitor Tablets contain EDTA and both irreversible and reversible protease inhibitors.

For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.

cOmplete™, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete, Mini contains EDTA (1 tablet yields a 1 mM EDTA solution in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete, Mini EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is present.
cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml) we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore comparable results are achieved with both product types.
cOmplete™, Mini Protease Inhibitor Cocktail has been used as a
component of

lysis buffer for lysing glioma tumor samples
radioimmunoprecipitation (RIPA) protein extraction buffer for homogenization of heart tissues
resuspension buffer for retinal ganglion cells homogenization

Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Preparation of Working Solution
One cOmplete Mini Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)

Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	vial of 25 tablets
Торговая марка	Roche
solubility	water: soluble
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™, Mini Protease Inhibitor Cocktail 4693124001

cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete, Mini, contains EDTA and both irreversible and reversible protease inhibitors.
Specificity
For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.

cOmplete™, Mini Protease Inhibitor Cocktail has been used as a component in

mammalian-protein extraction reagent (M-PER) for lysis of glioma stem cells (GSCs)
sodium dodecyl sulphate-tris buffered saline (SDS-TBS) for the homogenisation of brain tissues
radioimmunoprecipitation assay (RIPA) buffer for lysis of human embryonic stem cells (hESCs)

cOmplete™, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.
cOmplete, Mini, contains EDTA (1 tablet yields a 1 mM EDTA solution in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete, Mini, EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is present.
cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml), we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore, comparable results are achieved with both product types.
Features and Benefits

Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

Packaging
30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Quality
Performance-tested with pancreas extract.
Physical form
Tablets in foil blister packs
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution
Preparation of Working Solution:
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent breakage of tablets.
One cOmplete Mini tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. The tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.):
Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pack of 30 tablets (individually packed in foil blister packs)
Торговая марка	Roche
solubility	aqueous buffer: soluble, water: soluble
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail 11836170001

cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets, they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

For the inhibition of serine, cysteine, but not metalloproteases.

cOmplete™Mini, EDTA-free Protease Inhibitor Cocktail has used as a component of

resuspension buffer for nuclear lysates preparation from prostate cancer cell lines for chromatin and RNA immunoprecipitation
resuspension buffer for protein extraction from neurospheres for western blot analysis
homogenization buffer for protein extraction from rat left ventricle samples and immunoprecipitaion buffer for human embryonic kidney 293 (HEK293) cells

cOmplete™, Mini, EDTA-free protease inhibitor cocktail has been used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.

Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.

Proprietary mixture of several protease inhibitors.

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Preparation of Working Solution:

One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.):

Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	vial of 25 tablets
Торговая марка	Roche
solubility	aqueous buffer: soluble
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail 4693159001

cOmplete, Mini, EDTA-free tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).
Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.

cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail has been used as a component of

nonidet P-40 (NP-40) buffer for the preparation of cell lysate of neuroblastoma cells
tissue lysis buffer for homogenisation and lysis of prefrontal cortex tissues
ice-cold radioimmunoprecipitation assay (RIPA) buffer for the lysis of Chinese hamster ovary (CHO-K1) cells

cOmplete™, Mini, EDTA-free, is used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.

30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.

Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

Packaging

Tablets in foil blister packs

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Handling

Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution

One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)

Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C. Can be used in thiol-containing solutions at 15 to 25 °C.

Storage and Stability

Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)

For life science research only. Not for use in diagnostic procedures.

form	tablet
packaging	pkg of 30 tablets (individually packed in foil blister packs)
Торговая марка	Roche
concentration	(Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10 ml cell extract.)
solubility	aqueous buffer: soluble, water: soluble
absorption	0.08 at 280 nm
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

COT Human DNA 11581074001

The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
Repetitive elements (IRS) present in a probe (e.g., cosmids, YACs, chromosome painting probes) generate nonspecific hybridization signals that are distributed over the whole chromosome or genome. To enable specific hybridization of the probe to the chromosomal target site (e.g., single-copy sequences or low-copy repeats) the probe must be denatured in the presence of excess unlabeled COT Human DNA. This DNA serves as a competitor. In a subsequent preannealing step, the repetitive probe elements rapidly hybridize to excess repeats in the COT Human DNA, while most of the specific probe sequences remain single-stranded and thus can be hybridized to their chromosomal targets. This technique is known as chromosomal in situ suppression (CISS) hybridization.

COT Human DNA is used in chromosome in situ suppression (CISS) hybridization. Cosmid or YAC probes contain repetitive elements that result in monospecific hybridization signals distributed over the entire chromosome. To enable specific hybridization to the chromosomal target site, the probe is denatured together with an excess of unlabeled COT Human DNA as a competitor. COT Human DNA can be used to suppress nonspecific hybridization to human repetitive sequences in microarray analysis, and in filter and in fluorescent in situ hybridization experiments.
Sequence
In agarose gel electrophoresis the length distribution of the COT Human DNA fragments shows a maximum in the range of 50 to 300 nucleotides.
Physical form
Solution, 1 mg/ml, 10 mM Tris-HCl, 1 mM EDTA, pH 7.4

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 500 µg
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

COT Human DNA, Fluorometric Grade 5480647001

COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich highly repetitive elements. It can be used to suppress cross-hybridization to human repetitive DNA when human DNA probes are hybridized in situ. General applications are FISH and DNA microarray hybridizations such as comparative genome hybridization as well as all applications where complex human DNA is used as a target or probe during hybridization experiments.

COT Human DNA is used in the following applications:

Nucleic acid labeling and detection
DNA Microarray applications such as comparative genome hybridization and sequence capture
Complex hybridization of human nucleic acids like FISH

In microarray applications, COT Human DNA is used in hybridization solutions to block repetitive DNA sample sequences from nonspecific hybridizations. In filter and other hybridization techniques, COT Human DNA is also used in pre-hybridization solutions to inactivate nonspecific target binding sites. In all applications customers would like to suppress nonspecific signals most efficiently. The scientific field targeted with this product is for scientists interested in the analysis of genomic variations within the human genome.
Features and Benefits

Improve specificity by blocking unspecific nucleic acid motifs using high concentrations of double-stranded, repetitive, human DNA.
Achieve consistent and reliable results using fluorometrically quantified COT Human DNA, for preferentially detecting dsDNA.
Use COT Human DNA that has been tested using DNA microarrays and comparative genome hybridization (CGH).

Repetitive elements (IRS) present in a probe (e.g., cosmids, YACs, chromosome painting probes) generate nonspecific hybridization signals that are distributed over the whole chromosome or genome. To ensure specific hybridization of the probe to the chromosomal target site (e.g., single-copy sequences or low-copy repeats), the probe must be denatured in the presence of excess unlabeled COT Human DNA. COT Human DNA serves as a competitor. In a subsequent pre-annealing step, the repetitive probe elements rapidly hybridize to excess repeats in the COT Human DNA, while most of the specific probe sequences remain single-stranded, and can thus be hybridized to their chromosomal targets. This technique is known as chromosomal in situ suppression (CISS) hybridization.
Quality
The concentration of the COT Human DNA, Fluorometric Grade, is accurately measured to provide a consistent 1.0 to 1.5mg/ml by fluorometric determination using the Hoechst Dye 33258. Fluorometric analysis ensures a high concentration of repetitive DNA for a consistent, high concentration of active repetitive sequence motifs and optimal suppression of unspecific signals in complex nucleic acid hybridization experiments. Each lot is performance tested in a standard DNA microarray comparative genomic hybridization (CGH) experiment, which makes this product especially suitable for CGH and other DNA microarray applications. COT Human DNA, Fluorometric Grade has a shelf-life of 18 months.
For quality assessment in the CGH application, target sequences located on the X-chromosome are compared in male and female genomic DNA samples. The donor material for this product was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, HCV, and found to be negative.
Sequence
Maximum of length distribution in the range 50 to 300 nucleotides
Physical form
Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.2
Storage and Stability
Store at -15–-25 °C. (repeated freezing and thawing does not deteriorate the product)

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 1 mg
Торговая марка	Roche
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

CPRG 10884308001

Empirical Formula (Hill Notation):	C₂₅H₂₂Cl₂O₁₀S
CAS Number:	99792-79-7
Molecular Weight:	585.41
MDL number:	MFCD05863726
PubChem Substance ID:	329749219

Chlorophenol red--D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Colorimetric determination of β-Gal activity in cell extracts and ELISA.
Quality
Contaminant: <0.1% chlorophenol red
Sequence
CPRG is an approximately 1:1 mixture of the isomers.
Preparation Note
Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C
Storage and Stability
Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	96% (HPLC)
form	powder
packaging	pkg of 250 mg
Торговая марка	Roche
storage condition	protect from light
pH range	6 - 8
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)
SMILES string	OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
InChI	1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
InChI key	YOUWVNCQJOMMEU-AETRGLENSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Creatine Kinase (CK)

2.7.3.2 ( BRENDA | IUBMB )

ATP:creatine N-phosphotransferase
Creatine kinase (CK) has a molar mass of 85kDa. It exists as a dimer in the cytosolic form and as a dimer and an octamer in the mitochondrial form. The cytosolic forms are named as MM, BB, and MB, where M and B correspond to muscle and brain, respectively. CK from rabbit muscle is a dimer containing two identical polypeptide chains of 380 amino acids. Each subunit has four cysteines, Cys73, Cys145, Cys253 and Cys282, but lacks inter- and intra-chain disulfide bonds.

Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.
Biochem/physiol Actions
Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.
Quality
Contaminants: <0.001% ATPase, HK, and myokinase, each
Specifications
Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.
Unit Definition
One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.
Physical form
Lyophilizate, stabilized
Preparation Note
Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.
Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~350 units/mg protein (at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.)
packaging	pkg of 100 mg (10127566001), pkg of 500 mg (10736988001)
Торговая марка	Roche
optimum pH	6.0-7.0(reverse reaction), 9.0(forward reaction)
shipped in	wet ice
storage temp.	2-8°C

Creatine kinase is responsible for the production of ATP (adenosine triphosphate) by transferring phosphate from creatine phosphate to ADP (adenosine diphosphate). Creatine phosphate is provided as a disodium salt.

Creatine phosphate

Empirical Formula (Hill Notation):	C₄H₈N₃Na₂O₅P · 4H₂O
Linear Formula:	Na₂O₃PNHC(=NH)N(CH₃)CH₂COOH · 4H₂O
CAS Number:	71519-72-7
Molecular Weight:	327.14
MDL number:	MFCD00150192
PubChem Substance ID:	329798458

Creatine phosphate has been used in the energy regeneration mix for sperm, spermatids and egg extracts.
Quality
Purity: >97% creatine-P-Na2 x 4 H2O

For life science research only. Not for use in diagnostic procedures.

assay	>97%
Quality Level	100,
mol wt	211.1
packaging	pkg of 10 g (10621722001), pkg of 5 g (10621714001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C
SMILES string	O.O.O.O.[Na+].[Na+].CN(CC(O)=O)C(=N)NP([O-])([O-])=O
InChI	1S/C4H10N3O5P.2Na.4H2O/c1-7(2-3(8)9)4(5)6-13(10,11)12;;;;;;/h2H2,1H3,(H,8,9)(H4,5,6,10,11,12);;;41H2/q;2+1;;;;/p-2
InChI key	HUWYWJSJJDCZRQ-UHFFFAOYSA-L

Cytotoxicity Detection Kit (LDH) 11644793001

A nonradioactive alternative to the [51Cr] and [3H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.

The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:

Detection and quantification of cell-mediated cytotoxicity
Determination of mediator-induced cytolysis
Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries
Determination of cell death in bioreactors
Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Packaging
1 kit containing 2 components.
Preparation Note
Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 µl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 2,000 tests
Quality Level	100,
packaging	pkg of 1 kit
Торговая марка	Roche
application(s)	cell analysis: suitable, detection: suitable, protein quantification: suitable
_max	490 nm
detection method	colorimetric
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Nonradioactive colorimetric assay suitable for high-throughput quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant. The Cytotoxicity Detection KitPLUS (LDH) can be performed in a homogeneous format and requires no transfer and centrifugation steps to separate the supernatant from the cells. The color reaction can be stopped for defined assay conditions.

Cytotoxicity Detection KitPLUS (LDH)

The assay measures LDH activity released from damaged cells. It is performed in 96-well plates.
Features and Benefits

The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format. The kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example: Detection and quantification of cell-mediated cytotoxicity.
Determination of mediator-induced cytolysis.
Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries.
Determination of cell death in bioreactors.

Flexible, since the assay can determine the degree of plasma membrane damage in many different in vitro cell systems.
Safe, because the assay does not use radioactive isotopes.
Accurate, since assay results strongly correlate to the number of lysed cells.
Sensitive, since the assay can detect even low cell numbers.
Fast, since assay results can be determined on a multi-well ELISA reader, which allows simultaneous processing of many samples.
Convenient, since the assay does not require a prelabeling step.

Packaging

04744926001 (1 kit containing 4 components)
04744934001 (1 kit containing 4 components)

1 kit containing 4 components
Preparation Note
Working solution: Catalyst:
Dissolve the lyophilizate in 1 ml double dist. water for 10 min, then mix thoroughly.
Reaction mixture:

For 100 tests: Shortly before use, mix 250 µl of reconstituted catalyst with 11.25 ml dye solution
For 400 tests: Shortly before use, add the total volume of reconstituted catalyst (1 ml) to the total volume of dye solution (45 ml) and mix well.
Note: In case you observe crystals in “Dye solution”, shake the bottle at least 1 hour at 37 °C. Remaining precipitates will NOT influence the performance.
Note: The precipitates were formed during the freezing of the product. Repeated freezing and thawing should be avoided.
Note: Prepare immediately before use. Do not store the Reaction mixture.
Storage conditions (working solution): The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution, the dye solution the lysis solution and the stop solution are stable for

Four weeks when stored at 2 to 8 °C
Two days at 15 to 25 °C
For long-term storage (up to 3 months), store bottles at -15 to -25 °C.
Freezing and thawing the solutions up to three times will not significantly reduce their performance. When crystals are seen in Dye solution, shake the bottle at least one hour at 37 °C. Remaining precipitates will not influence performance. As the precipitates can be formed during freezing of the product, repeated freezing and thawing should be avoided.

For life science research only. Not for use in diagnostic procedures.

form	liquid
Quality Level	100,
usage	sufficient for 2,000 multiwell tests (04744934001), sufficient for 400 multiwell tests (04744926001)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
application(s)	cell analysis: suitable, detection: suitable, protein quantification: suitable
_max	490-492 nm
detection method	colorimetric
shipped in	dry ice
storage temp.	20°C

DAB Substrate 11718096001

The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3 diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.

DAB substrate has been used in immunofluorescence and western blot.
Packaging
1 set containing 2 components.
Preparation Note
Working solution:

DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
In situ hybridization
Western blot
Dot blot

Determine the volume of substrate required for the development of peroxidase.
Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
Note: Do not bring to 15 to 25 °C.
Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 µl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
Mix well.
Add the 1x working solution to the tissue or blot until desired substrate development.
Note: The 1x solution is stable over many hours.
For best results, store the working solution at 2 to 8 °C when not using.

Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
color	dark brown, /black precipitate; Visually evaluated
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS02,GHS06,GHS08,GHS09
signalword	Danger
hcodes	H225 - H301 + H311 + H331 - H317 - H350 - H350i - H360FD - H370 - H373 - H411
pcodes	P201 - P210 - P260 - P273 - P280 - P301 + P310 + P330 - P308 + P311 - P370 + P378 - P391 - P403 + P233
RIDADR	UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany	WGK 3

DAPI 10236276001

Empirical Formula (Hill Notation):	C₁₆H₁₅N₅ · 2HCl
CAS Number:	28718-90-3
Molecular Weight:	350.25
Beilstein/REAXYS Number:	4894417
MDL number:	MFCD00012681
PubChem Substance ID:	329748990

DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Quality
Purity: >90% (from N)
Principle
The fluorescent dye DAPI binds selectively to DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces.

Preparation Note
Working solution: Solubility: 25 mg/ml in water

Preparation of stock solution

Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.

Preparation of working solution

Dilute the stock solution with methanol to a final concentration of 1 µg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1µg/ml) at 2 to 8 °C for about 6 months.
Reconstitution
In 2 to 10 ml double-dist. water; 1 to 5 mg/ml final concentration.
Note: Prepare aliquots and store at -15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>90%
form	powder
packaging	pkg of 10 mg
Торговая марка	Roche
_max	340 nm in aq. suspension
fluorescence	_ex 340 nm; _em 488 nm (nur DAPI), _ex 364 nm; _em 454 nm (DAPI-DNA-Komplex)
shipped in	ambient
storage temp.	room temp
SMILES string	Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI	1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key	FPNZBYLXNYPRLR-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

Dideoxynucleoside Triphosphate Set 3732738001

The Dideoxynucleoside Triphosphate Set, Sequencing Grade, sodium salt solution, consists of dideoxynucleotides of very high purity (ddNTP HPLC, area% 98%; ddNDP HPLC, area% 1.5%), specifically manufactured and tested for sequencing reactions.
The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).

2,3-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase (e.g., Klenow enzyme) and are therefore used for DNA sequencing according to Sanger (Sanger, F. et al. (1977) PNAS USA 74, 5463).
Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T).
Packaging
The set contains 4 individual vials of ddATP, ddCTP, ddGTP and ddTTP each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).
Quality
Each lot of dideoxynucleotide solution is assayed for function in sequencing reaction.
Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Preparation Note
Activator: Mg2+, Co2+ and Mn2+ ions
Working solution: Preparation of Termination Mix
Before preparing the termination mix, dilute an appropriate volume of each dideoxynucleotide in water to a concentration of 10 µM.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	1.5% (ddNDP, HPLC), 98% (ddNTP, HPLC)
packaging	pkg of 4 x 100 μL (4 x 1 μmol; 10 mM, each)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DIG Easy Hyb™ 11603558001

DIG Easy Hyb is a nontoxic, ready-to-use hybridization buffer. DIG Easy Hyb does not contain formamide, but facilitates lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DIG Easy Hyb can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. DIG Easy Hyb is ready-made, and can be used without any additions. It is specifically designed for use with DIG kits.
Quality
Absence of contaminants: DNase- and RNase-free.

For life science research only. Not for use in diagnostic procedures.

DIG Easy Hyb is a trademark of Roche

form	solution
Quality Level	100,
packaging	pkg of 500 mL
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H318
pcodes	P280 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	No data available
Flash Point C	No data available

DIG Easy Hyb™ Granules 11796895001

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.
Preparation Note
Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

For life science research only. Not for use in diagnostic procedures.

DIG Easy Hyb is a trademark of Roche

form	powder
Quality Level	100,
usage	sufficient for 6 x 100 mL
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	ambient
storage temp.	20-25°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

DIG RNA Labeling Kit (SP6/T7) 11175025910

Kit for the labeling of RNA with digoxigenin-UTP by in vitro transcription with SP6 and T7 polymerases. By this method, single-stranded RNA probes of known length are produced, which can be used in a variety of hybridization techniques.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Assay Time: 145minutes
Sample Materials

Linearized plasmid DNA
PCR product

Principle
The DIG RNA Labeling Kit produces DIG-labeled, single-stranded RNA probes of known length. Either SP6 or T7 RNA polymerase transcribes these probes in vitro from template DNA (in the presence of digoxigenin-UTP).
RNA Labeling by in vitro Transcription
The DNA to be transcribed is cloned into the polylinker site of appropriate transcription vectors (e.g., pSPT 18 or 19), which contain promoters for SP6 and T7 RNA polymerases. Adjacent template DNA is linearized at a suitable site and the RNA polymerases are used to produce "run off" transcripts. DIG-UTP is incorporated into the transcript. Every 20 to 25th nucleotide of the newly synthesized RNA is a DIG-UTP. Since the nucleotide concentration does not become limiting in the standard transcription reaction, this reaction can generate large amounts of labeled RNA.
Specificity
Sensitivity and Specificity
DIG-labeled RNA probes can detect single-copy genes in as little as 1 µg of mammalian DNA under the following assay conditions: The hybridization mix contains 20 to 100 ng labeled probe/ ml, and the bound probe is detected with anti-DIG-AP and visualized with the chemiluminescent substrate CDP-Star.
Heat inactivation: Stop the reaction by adding 2 µl 0.2 M EDTA (pH 8.0).

For RNA labeling with DIG-11-UTP by in vitro transcription with SP6 and T7 RNA Polymerases. DIG-labeled "run off" transcripts are synthesized with high efficiency and can be used in a variety of hybridization techniques:

Northern blots
Southern blots
In situ hybridizations
Plaque or colony lifts
RNase protection experiments

Due to highly specific and sensitive detection systems, DIG-labeled probes can be used for single-copy gene detection in 1µg total human DNA.
Note: Since the linkage between DIG and UTP is resistant to alkali, DIG-labeled RNA can be fragmented by alkaline treatment. Slightly reducing the size of the DIG-labeled RNA probe may make it more suitable for certain applications in in situ hybridization.
Packaging
1 kit containing 12 components.
Quality
Test principle: The DNA template to be transcribed is cloned into the polylinker site of appropriate transcription vectors, which contain promoters for SP6 and/or T7 RNA Polymerases. After linearization at a suitable site, RNA is transcribed in the presence of DIG-UTP. Under standard conditions, approximately 10μg of full-length DIG-labeled RNA is transcribed from 1μg template.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 2 x 10 labeling reactions
Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H302 - H319
pcodes	P264 - P270 - P280 - P301 + P312 + P330 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

DIG RNA Labeling Mix 11277073910

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Labeling efficiency: Approximately 10µg of full-length digoxigenin-labeled RNA is transcribed from 1µg linear template DNA.
Assay Time: 135 minutes
Sample Materials
Linearized plasmid DNA:
The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of ‘run off transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5-overhangs should be used; 3 overhangs should be avoided. The linearized template DNA should be purified by phenol chloroform extraction and ethanol precipitation, to avoid RNase contamination. For run around transcription circular plasmid DNA is used.
PCR product:
PCR-fragments which contain RNA polymerase promoter sequences can also be used as templates for transcription. Purification of the PCR fragment by HighPure column purification prior to transcription is recommended.
DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. DIG-11-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The DIG RNA Labeling Mix is specifically designed for the use with SP6, T7, and T3 RNA polymerases, which are supplied with an optimized transcription buffer.
Convenient nucleotide mixture for the labeling of RNA with Digoxigenin-11-UTP.
Contents
10x solution with: 10 mM ATP, CTP, GTP (each), 6.5 mM UTP, 3.5 mM DIG-11-UTP.
Specificity
Heat inactivation: Stop the reaction by adding 2 µl 0.2 M EDTA (pH 8.0).

RNA labeling with Digoxigenin-11-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. DIG-labeled RNA is used in a variety of hybridization techniques:

Northern blots
Southern blots
Dot blots
Plaque or colony lifts
RNase protection experiments
Chromosomes, cells, and tissue sections in situ

Features and Benefits
The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer.
Quality
Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 20 reactions
packaging	pkg of 40 µL
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H302
pcodes	P264 - P270 - P301 + P312 + P330 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

DIG Wash and Block Buffer Set 11585762001

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.
Packaging
1 set containing 4 components.
Quality
The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.
Preparation Note
Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 µl dATP, (vial 2)
1 µl dGTP, (vial 4)
1 µl dTTP, (vial 5)
to a reaction vial.
Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):

After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

DIG-High Prime DNA Labeling and Detection Starter Kit I 11745832910

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DIG-High Prime DNA Labeling and Detection Starter Kit I has been used in a variety of hybridization techniques:

in Southern blots
in northern blots
in dot blots
in colony and plaque hybridizations
for all types of filter hybridization
for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley and wheat

DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
Features and Benefits
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 principles of greener chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG system, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Packaging
1 kit containing 7 components.
Quality
Function tested in a dot blot.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 12 labeling reactions, sufficient for 24 blots
Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

DIG-High Prime DNA Labeling and Detection Starter Kit II 11585614910

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:

in Southern blots
in northern blots
in dot blots
in colony and plaque hybridizations
for all types of filter hybridization
for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Packaging
1 kit containing 7 components.
Principle
The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 12 labeling reactions (10 ng to 3 µg per assay), sufficient for 24 blots (blots of 100 cm²)
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Digoxigenin-11-ddUTP 11363905910

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Digoxigenin-11-ddUTP has been used:

for labeling of DNA fragments in linker histone binding assay
for end labeling of NF-B (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions
Quality
Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C43H61N4O20P3Li4

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	85% (HPLC)
form	solution
mol wt	(Molecular weight: M_r = 1074.7 (DIG-11-ddUTP-Li₄))
packaging	pkg of 25 µL (25 nmol; 1mM)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.

Digoxigenin-11-dUTP, alkali-stable

Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Features and Benefits
Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage.
Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.
Quality
Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%).
Function tested by RPL.
Preparation Note
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 µl nucleotide mix add 10 µl of dATP, dCTP, dGTP, dTTP (each) to 60 µl Water, PCR Grade).
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	85% (HPLC)
form	solution
mol wt	M_r 1090.7
packaging	pkg of 125 µL (11558706910 [1 mM]), pkg of 5 x 125 µL (11570013910 [5x1 mM]), pkg of 25 µL (11093088910 [1 mM])
Торговая марка	Roche
_max	222 and 292 nm, 22430 and 8780
shipped in	dry ice
storage temp.	20°C

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH2-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Digoxigenin-3-O-methylcarbonyl--aminocaproic acid-N-hydroxysuccinimide ester

Empirical Formula (Hill Notation):	C₃₅H₅₀N₂O₁₀
CAS Number:	129273-26-3
Molecular Weight:	658.78
MDL number:	MFCD02093394
PubChem Substance ID:	329749387

Digoxigenin-3-O-methylcarbonyl--aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).
Biochem/physiol Actions
Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.
Principle
The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.
Preparation Note
Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- -aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 µl DMSO or ethanol.

For life science research only. Not for use in diagnostic procedures.

assay	>90% (elementary analysis)
Quality Level	100,
form	powder
mol wt	M_r 658.8
packaging	pkg of 5 mg
Торговая марка	Roche
pH range	2 - 13
solubility	DMF: soluble, DMSO: soluble, ethanol: soluble
shipped in	dry ice
storage temp.	20°C
SMILES string	[H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
InChI	1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
InChI key	KHNDABJZSPPYLE-FUGFVFQCSA-N

pictograms	GHS06,GHS08,GHS09
signalword	Danger
hcodes	H300 - H319 - H331 - H373 - H410
pcodes	P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
RIDADR	UN 2811 6.1 / PGI
Flash Point F	Not applicable
Flash Point C	Not applicable

Dispase® I (neutral protease, grade I) 4942086001

3.4.24.4 ( BRENDA | IUBMB )

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.
Specificity
Dispase® is a non-specific metalloprotease.

Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
Features and Benefits

Rapid, effective, yet gentle agent that liberates intact cells
Maintains cell membrane integrity
Non-mammalian source - free of mycoplasma and animal virus contamination
Extremely stable to influences of temperature, pH, and interference by serum components
Easily inactivated by chelating agents or by dilution
Delivers higher activity and convenience

Contents
10 vials with approximately 2mg each (= 20U/vial).
Specifications
Enzyme activity: =10U/mg (+37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Unit Definition
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides from casein corresponding to 1 µmol (181 µg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of RAS units of Dispase® with those cited in the Japanese literature (where concentrations of 1,000 to 2,000 Dispase® units /ml are not uncommon) suggests one Roche Applied Science unit of Dispase® I equals approximately 416 Japanese units of Dispase®.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 µg tyrosine per min and ml at pH 7.5 and +37 °C.
Physical form
white lyophilizate, before lyophilization filtered through 0.2 μm pore size membrane
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® I enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 µm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months, the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing.
The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Quality Level	100,
form	lyophilized
specific activity	10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5))
packaging	pkg of 10 x ~2 mg
Торговая марка	Roche
optimum pH	6.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Dispase® II (neutral protease, grade II) 4942078001

3.4.24.4 ( BRENDA | IUBMB )

Unit Definition
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol (181 µg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 µg tyrosine per min and ml at pH 7.5 and +37 °C.
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 µm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
Maintains cell membrane integrity
Non-mammalian source - free of mycoplasma and animal virus contamination
Extremely stable to influences of temperature, pH, and interference by serum components
Easily inactivated by chelating agents or by dilution
Delivers higher activity and convenience

For life science research only. Not for use in diagnostic procedures.

Dispase is a registered trademark of Godo Shusei Co., Ltd.

sterility	non-sterile
Quality Level	100,
form	lyophilized
specific activity	0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)
packaging	pkg of 5 x 1 g
Торговая марка	Roche
optimum pH	6.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

DNA Fragmentation Imaging Kit 6432344001

The DNA Fragmentation Imaging Kit is a simple and rapid TUNEL assay for detecting apoptosis induction in mammalian cells. DNA fragmentation, detected using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP, is measured by fluorescence microscopy or an automated imaging platform, such as the Cellavista System. The kit uses a dye to stain the nuclei of living and dead cells for quantifying total and apoptotic cell numbers, and calculating the percentage of apoptotic cells.

Programmed cell death (apoptosis) is a key pathway in mammalian cells during tissue homeostasis. Cell death malfunction has been implicated in pathology. Quantification of caspase-triggered DNA fragmentation is one of the principal techniques used to detect apoptosis induction. The DNA Fragmentation Imaging Kit performs the TUNEL assay for accurate and fast quantitative fluorescence detection using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP. The kit is ideal for measuring apoptosis in medium to high throughput cellular workflows.

Quickly determine apoptosis induction for cell-based workflows in life science research.
Reliably perform screening assays in product development, e.g., cancer research, pre-clinical safety.
Accurately measure malignant cell sensitivity in cancer research.

Packaging
1 kit containing 3 components
Quality
The kit is function tested using a cellular model (HeLa cells treated with antimycin A).
Principle
Labeling of apoptotic cells
Genomic DNA cleavage during apoptosis produces double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), as well as single strand breaks (nicks) in high molecular weight DNA. These DNA strand breaks are identified by labeling free 3-OH termini with modified nucleotides in an enzymatic assay known as the TUNEL reaction. The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. TUNEL assays discriminate apoptosis from necrosis, and from primary DNA strand breaks induced by cytostatic drugs or irradiation. The DNA Fragmentation Imaging Kit labels DNA strand breaks using terminal deoxynucleotidyl transferase (TdT). TdT catalyzes polymerization of labeled nucleotides to free 3-OH DNA ends in a template-independent manner using the TUNEL reaction. Fluorescein incorporated in nucleotide polymers is detected and quantified using fluorescence microscopy.
Staining of nuclei
Hoechst 33342 is a cell-permeable stain that binds DNA. It is used to stain nuclei of living or dead cells.
Preparation Note
Storage conditions (working solution): The Reaction Solution created by mixing the Enzyme Solution and Label Solution (Vial 2 and Vial 3), in Step 6 of the experiment protocol, cannot be stored. The Reaction Solution should be prepared just before use. Discard remaining unused Reaction Solution.
Storage and Stability
Store at 15 to 25 °C. (Protect from exposure to light. Store dry in dark.)

Optimized for data evaluation with Roches Cellavista System. Obtain reproducible results using automated imaging with this system.
Saves time and resources: Total assay time, including analysis, for a 96-well microplate is 85 minutes.
Reduced risk of losing cells with fewer washing steps in an improved protocol.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice

pictograms	GHS08,GHS09
signalword	Danger
hcodes	H341 - H350i - H411
pcodes	P201 - P273 - P280 - P308 + P313 - P391 - P501
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

DNA Isolation Kit for Cells and Tissues 11814770001

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:

Genomic Southern blotting
Sequencing
Restriction digestion
PCR/long PCR
Cloning

Features and Benefits
Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.
Components

Cellular Lysis Buffer
Proteinase K Solution
RNase Solution
Protein Precipitation Solution

Quality
Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.
Preparation Note
Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.

Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse 2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse -2 collagen gene fragments (5.6 kb and 10.4 kb) and human -globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
For life science research only. Not for use in diagnostic procedures.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 10 isolations

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

DNA Isolation Kit for Mammalian Blood 11667327001

Isolation of DNA from whole blood can be difficult because blood is a complex mixture containing cells, proteins, metabolites, etc. Most of the cells (>99%) are erythrocytes (red blood cells) which lack nuclei, and therefore, possess no DNA. Leukocytes (white blood cells) contain nuclei and DNA. Therefore, DNA from blood must be isolated from one of three types of leukocytes: monocytes, lymphocytes, or granulocytes.
Sample material:
Human whole blood (treated with sodium heparin, EDTA or sodium citrate): 1 to 10 mL
Isolated lymphocytes: 1 to 10 mL
Isolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples.

DNA Isolation Kit for Mammalian Blood has been used in isolation of genomic DNA from blood of asthmatic patients for single nucleotide polymorphisms genotyping.
The DNA Isolation Kit for Mammalian Blood purifies DNA that is suitable for most molecular biology applications:

PCR/long PCR
Sequencing
Restriction digestion
Southern blotting
Cloning

DNA is easily purified in <90 minutes (plus 30 to 60 minutes resuspension time).

Process multiple samples simultaneously.

Use a cost-effective kit.

Achieve faster DNA purification for the cost of most homebrew methods.

Obtain consistent and reliable results.

Analyze different sample volumes (1 to 10 mL) with varying amounts of leukocytes.

Improve safety.

Eliminate the use of chaotropic salts, hazardous organic solvents, and column purification steps.
Components

Red Blood Cell Lysis Buffer
White Blood Cell Lysis Buffer
Protein Precipitation Solution

Quality
Each lot of kits is function tested for the ability to purify DNA from human whole blood, followed by specific amplification of a 4.8 kb tPA fragment via PCR using the Expand Long Template PCR System. The 4.8 kb tPA product is visualized by electrophoresis on an agarose gel and compared to appropriate positive and negative controls. A single 4.8 kb tPA band of equivalent intensity to the positive control is visible. The kit buffers are also tested for the absence of DNase contamination. Each solution is incubated with 1 μg pBR322 DNA for 6 hours at +37°C. The DNA is then visualized by electrophoresis on an agarose gel, and then compared to a positive control to determine if any linear or nicked plasmid DNA is visible.
Preparation Note
The DNA Isolation Kit for Mammalian Blood procedure relies on the separation of white blood cells from whole blood via a preferential red blood cell lysis. In the presence of a strong anionic detergent, the white blood cells are lysed, and then the proteins are removed by dehydration and precipitation. The purified DNA is subsequently recovered via ethanol precipitation.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 25 reactions

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker II 10236250001

DNA Molecular Weight Marker II is a ready-to-use solution, 250µg/ml, in TE buffer (Tris-HCl, 1mM EDTA, pH 8.0). The bacteriophage DNA is fragmented in a restriction digestion with Hind III endonuclease. The digestion reaction results in eight double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130. The fragments have 5-protruding ends and can be labeled with radioactive nucleotides (e.g., [32P]-dTTP or [32P]-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase. The product is also available as DNA Molecular Weight Marker II, Digoxigenin-labeled.
The High Fidelity PCR Master is a ready-to-use, double-concentrated master mix that contains all the reagents (PCR-Grade dNTPs, MgCl2, and an optimized reaction buffer) required to perform PCR (except template and primer). PCR grade water is also supplied.
The special enzyme blend is composed of two thermostable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. The 53 polymerase activity of Taq DNA Polymerase and the 35 exonuclease of the proofreading polymerase combine for improved performance. It gives PCR products with high yield, high fidelity, and high specificity from episomal and genomic DNA. Due to the inherent 35 exonuclease activity of the proofreading polymerase, the High Fidelity PCR Master results in approximately three times the fidelity and approximately twice the yield of Taq DNA Polymerase alone. The product is ideal for blunt end cloning or T/A cloning. It can amplify longer fragments (up to 5kb) with high fidelity and also minimize contamination with the help of two pipetting steps.

DNA Molecular Weight Marker II provides accurate sizing of fragments over a broad range of sizes. The fragments have 5-protruding ends and can be labeled with radioactive nucleotides (e.g. [32P]-dTTP or [32P]-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

DNA molecular weight marker II has been used to estimate the nucleic acid concentrations corresponding to the intensities of fluorescence obtained.
The High Fidelity PCR Master is used for:

PCR up to 5kb
RT-PCR up to 5kb

DNA Molecular Weight Marker II provides accurate sizing of fragments over a broad range of sizes. It is used as a DNA Molecular Weight Marker for agarose gels and simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

Features and Benefits
The High Fidelity PCR Master reduces the setup time and can be immediately used without thawing.
Sequence
The bacteriophage lambda () DNA is fragmented in a restriction digestion with Hind III endonuclease. The digestion reaction results in eight double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 µg = 1A260 unit
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed store at 2 to 8 °C.
Storage and Stability
Store at -15–-25 °C. (unopened vial)

Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker III 10528552001

Contents
Ready-to-use solution, 250 µg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
The bacteriophage DNA is fragmented in a restriction digestion with EcoR I and Hind III endonuclease.
The product is also available as DNA Molecular Weight Marker III, Digoxigenin-labeled.
Size Range: 0.12 to 21.2 kbp

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

DNA Molecular Weight Marker III provides accurate sizing of fragments over a broad range of sizes. The fragments have 5-protruding ends and can be labeled with radioactive nucleotides (e.g., [32P]-dTTP or [32P]-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
Sequence
The digestion reaction results in thirteen double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 125, 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, and 21226 as determined by computer analysis of the DNA sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 µg = 1A260 unit
Preparation Note
Working concentration: 1 to 3 mg/ml buffer
Working solution: Recommended solvent is 25 mM Tris/HCl, 300 mM NaCl, 1mM EDTA pH 8.0.
Product is stable in 5 mM urea and 0.1% SDS (w/v).
Storage conditions (working solution): 2 to 8 °C
Once thawed, store at 2 to 8 °C.
Storage and Stability
Store at -15–-25 °C. (until use)

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker IV 11418009001

Fragment mixture prepared by cleavage of equimolar amounts of DNA and pSPTBM20 DNA with restriction endonucleases Sty I and Sau I.
Ready-to-use solution, 250 µg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Size Range: 0.07 to 19.3 kbp

DNA Molecular Weight Marker IV is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

(Use this marker to size DNA fragments between 0.07 and 19.3 kbp).
Sequence
The mixture contains 14 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 74, 421, 697, 925, 1150, 1489, 1882, 2322, 2690, 3140, 4254, 5526, 7743, and 19329 as determined by computer analysis of DNA and pSPTBM 20 sequences.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed, store at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker IX 11449460001

DNA Molecular Weight Marker IX contains a mixture of eleven DNA fragments. The mixture is obtained by cleavage of X 174 DNA with restriction endonuclease Hae III.
Size Range: 72 to 1353 bp

DNA Molecular Weight Marker IX is especially designed for the analysis of mid-size PCR products in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

Sequence
The mixture contains eleven DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 µg = 1A260 unit
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker V 10821705001

Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III.
Size Range: 8 to 587 bp

DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
<UL><LI>Restriction digests</LI>
<LI>PCR</LI>
<LI>RT-PCR</LI></UL>
Components
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)
Sequence
The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 µg = 1A260 unit
Preparation Note
Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Storage and Stability
Store at -15–-25 °C. (until use)

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

DNA Molecular Weight Marker VI 11062590001

DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
Specificity
Specificity: Product has 5-phosphorylated ends (restriction fragments).
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp

DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

Restriction digests
PCR
RT-PCR

Sequence
As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 µg = 1A260 unit
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash