Molecular Biology &amp; Functional Genomics

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Quality
Performance test: incubation with AP
Substrates
Histochemical substrate for alkaline phosphatase.
Analysis Note
Absorption: Absorption maximum in the visible light is not determined.

For life science research only. Not for use in diagnostic procedures.

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	100,
assay	99% (HPLC)
form	powder
mol wt	M_r 326.44 (BCIP), M_r 433.6 (BCIP toluidine salt)
packaging	pkg of 1 g (11585002001), pkg of 250 mg (10760994001)
Торговая марка	Roche
solubility	DMF: soluble
shipped in	wet ice
storage temp.	2-8°C
SMILES string	Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI	1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key	QEIFSLUFHRCVQL-UHFFFAOYSA-N

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Bestatin 10874515001

Empirical Formula (Hill Notation):	C₁₆H₂₄N₂O₄ · HCl
CAS Number:	65391-42-6
Molecular Weight:	344.83
Beilstein/REAXYS Number:	4628066
MDL number:	MFCD00058004
PubChem Substance ID:	329749214

Bestatin has been used as a protease inhibitor for the purification of his-tagged Tau protein.
Biochem/physiol Actions
A metalloprotease inhibitor selective for aminopeptidase. Bestatin is a competitive and specific inhibitor of leucine aminopeptidase, aminopeptidase B, and triamino peptidase. It inhibits aminopeptidase B at 60 nM (using arginine--naphthylamide as substrate) and leucine aminopeptidase at 20 nM (leucine--naphthylamide as substrate). It showed no inhibition of aminopeptidase A, trypsin, chymotrypsin, elastase, papain, pepsin, or themolysin. It offers promise as a novel analgesic because it protects endogenous opioid peptides against degradation.
Bestatin exhibits anti-angiogenic property and inhibits new blood vessel formation at the early stage of tumor growth and in the metastatic sites.
Preparation Note
Working concentration: 40 µg/ml (130 µM)
Working solution: Recommended solvents are 1 M HCl, methanol or 0.15 M NaCl.
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl.
For best results, use a stock solution of 2–5 mg/ml in methanol.
Storage conditions (working solution): -15 to -25 °C
Avoid repeated freezing!
Reconstitution
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl. Roche recommends preparing a stock solution of 2 to 5 mg/ml in methanol.
Solutions are stable for 6 months when stored in aliquots at -15 to -25 °C.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	95% (HPLC)
form	crystalline powder
packaging	pkg of 10 mg
Торговая марка	Roche
storage condition	protect from light
mp	195-205 °C
solubility	water: soluble 25 g/L, alcohol: soluble 5 g/L
_max	255-260 nm at 1 N in hydrochloric acid
shipped in	wet ice
storage temp.	2-8°C
SMILES string	O=C(N[C@@H](CC(C)C)C(O)=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1.Cl[H]
InChI	1S/C16H24N2O4.ClH/c1-10(2)8-13(16(21)22)18-15(20)14(19)12(17)9-11-6-4-3-5-7-11;/h3-7,10,12-14,19H,8-9,17H2,1-2H3,(H,18,20)(H,21,22);1H/t12-,13+,14+;/m1./s1
InChI key	XGDFITZJGKUSDK-UDYGKFQRSA-N

Bln I recognizes the sequence CCTAGG and generates fragments with 5-cohesive termini.
Compatible ends
Bln I ends are compatible with ends generated by Nhe I, Spe I and Xba I.
Isoschizomers
Bln I is an isoschizomer of Avr II.
Note: The complete 13 site Avr II restriction map of the E.coli genome has been reported.
Methylation sensitivity
The enzyme is not known to be affected by methylation.
Specificity
Recognition sites: CCTAGG
CCTAGG
Restriction site: CCTAGG
CCTAGG
Heat inactivation: No inactivation of Bln I after incubation at 65 °C for 15 minutes.
Quality
Absence of nonspecific endonuclease activities
1 µg DNA is incubated for 16 hours in 50 µl SuRE/Cut Buffer H with an excess of Bln I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5 µg [3H] labeled calf thymus DNA are incubated with 3 µl Bln I for 4 hours at +37°C in a total volume of 100 µl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Typical ligation and recutting assay
Bln I fragments obtained by complete digestion of 1 µg x EcoR I DNA ligated for 16 hours at +4°C with 1 U T4 DNA Ligase in 10 µl buffer that contains 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Bln I and EcoR I (yielding the typical pattern of x EcoR I x Bln I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Bln I (Avr II)

: 2
X174: 0
Ad2: 2
M13mp7: 0
M13mp18:0
pBR322: 0
pBR328: 0
pUC18: 0
SV40: 2

Unit Definition
One unit is the enzyme activity that completely cleaves 1 µg x EcoR I DNA fragments in one hour at +37 °C in a total volume of 25 µl (1x) SuRE/Cut Buffer H.
Analysis Note
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 25-50%
B: 50-75%
H: 100%
L: 0-10%
M: 25-50%

Activity in PCR buffer: Not tested
PFGE tested
Bln I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E.coli C 600) embedded in agarose for PFGE analysis, we recommend using 10 U of enzyme/µg DNA and 4 hour incubation.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1,000 U (11558170001 [10 U/µl]), pkg of 200 U (11558161001 [10 U/µl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

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BM Blue POD Substrate, soluble 11484281001

Empirical Formula (Hill Notation):	C₁₆H₂₀N₂
CAS Number:	54827-17-7
Molecular Weight:	240.34
Beilstein/REAXYS Number:	2808541
MDL number:	MFCD00007748
PubChem Substance ID:	329749453

BM Blue POD Substrate, soluble, is ideal for ELISA applications. It has been used in fluorescein-tagged exendin-4 binding assay.
Specifications
Formula: C16H20N2
Color: blue; yellow after reaction is stopped
Evaluation: photometric (450 nm)
Physical form
Solution, ready-to-use
Analysis Note
Absorption: Identical to TMB absorption spectrum

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution (ready-to-use)
mol wt	M_r 240.35
packaging	pkg of 100 mL
Торговая марка	Roche
solubility	water: soluble
shipped in	wet ice
storage temp.	2-8°C
SMILES string	Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key	UAIUNKRWKOVEES-UHFFFAOYSA-N

Packaging
1 kit containing 4 components.
Principle
Each lot is function tested on a dot blot.
The BM Chemiluminescence Western blotting System is designed for peroxidase-labeled secondary antibodies and the substrate luminol. In the presence of hydrogen peroxide (H202), horseradish peroxidase (HRP) catalyzes the oxidation of diacylhydrazides, such as luminol. An activated intermediate reaction product is formed, which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by 4-iodophenol. This acts as a radical transmitter between the formed oxygen radical and luminol.
Preparation Note
Working solution: Preparation of additional reagents and solutions
The table inside the PI describes the preparation of working solutions. For reproducible results equilibrate all solutions to 20 to 25 °C before use.
Storage conditions (working solution):

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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1% Blocking solution: -15 to -25 °C
0.5% Blocking solution: -15 to -25 °C
POD-labeled secondary antibody stock solution: 12 month at 2 to 8 °C
POD-labeled secondary antibody working solution: prepare always fresh
Detection solution: 1 week at 2 to 8 °C

Reconstitution
Reconstitute lyophilizate in 100 µl double-dist. water. This solution is stable for 12 months at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

BM chemiluminescence western blotting substrate (POD) has been used along with horseradish peroxidase-coupled secondary antibodies, to visualize cells on the membranes.
Features and Benefits
Sensitivity: <10pg antigen in western blots. Shows at least 10-fold higher sensitivity than other nonradioactive or radioactive detection systems on PVDF membranes (using -galactosidase, anti--galactosidase, or anti-mouse IgG-POD, Fab fragments).

Packaging
1 kit containing 3 components
Quality
Each lot is function tested in a dot blot.

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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BM Chemiluminescence Western Blotting Substrate (POD)

For life science research only. Not for use in diagnostic procedures.

packaging	pkg of 1 set (11500694001 [4,000 cm² membrane]), pkg of 1 set (11500708001 [1,000 cm² membrane])
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

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BM Condimed H1 11088947001

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Cell Line Origin
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
Features and Benefits
Contents
Solution in RPMI 1640, filtered through 0.2µm pore size membrane
Physical form
Solution in RPMI 1640, filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.
Analysis Note
Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	0.2 μm filtered
form	solution
packaging	pkg of 100 mL (10x)
Торговая марка	Roche
application(s)	cell culture \| hybridoma: suitable
solubility	water: miscible
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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BM-Condimed 10663573001

BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.

BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.
Physical form
Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate.
Preparation Note
Working concentration: 1 to 100µg/ml
1 to 100µg/ml
Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
Storage and Stability
Store at 2 to 8 °C. (Store in dark!)

For life science research only. Not for use in diagnostic procedures.

sterility	0.2 μm filtered
Quality Level	100,
form	solution
packaging	pkg of 100 mL
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
solubility	water: miscible
shipped in	wet ice

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

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BM-Cyclin 10799050001

BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.
Specificity
Sensitive Organisms: Mycoplasmas and bacteria

BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
Mycoplasma arginini,
Mycoplasma hyorhinis, and
Mycoplasma orale

from experimentally contaminated and chronically infected cell lines.
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.

BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.
Packaging
Set of two different antibiotics
Physical form
Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Preparation Note
Working concentration: 10µg/ml pleuromutilin derivative (BM-cyclin 1), 5µg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1µg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 37.5 mg (for 2 x 2.5 l medium)
Торговая марка	Roche
Mode of action	protein synthesis \| interferes
antibiotic activity spectrum	mycoplasma
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H302 - H315 - H319 - H335
pcodes	P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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BM-Purple 11442074001

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purple

Components
Ready-to-use solution.
Preparation Note
Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.

Colorimetric AP substrate for: Immunohistocytochemistry
Western blot
Southern blot
Northern blot
Colony and plaque hybridization
In situ hybridization

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 100 mL
Торговая марка	Roche
color	dark purple
solubility	H₂O: insoluble
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Bovine Serum Albumin 10711454001

The product, bovine serum albumin, is of special quality for molecular biology. Bovine serum albumin has homology with human serum albumin. Serum albumins are soluble proteins of the circulatory system and participate in various cellular processes.
Contents
Solution of 20mg/ml Bovine Serum Albumin in 50mM Tris-HCl, 0.1M NaCl, 0.25mM EDTA, 1mM 2-mercaptoethanol, 50% glycerol (v/v), pH approximately 7.6.

Bovine Serum Albumin supports enzyme stability. It has been used for the:

preparation of circular DNA molecules
amplification of bacterial 16S rRNA genes
BOX-PCR fingerprinting

Quality
Contaminants: Albumin is checked for the absence of DNases, nicking activity, RNases, and proteases according to the current Quality Control procedures.
Physical form
Solution of 20 mg/ml Bovine Serum Albumin in 50 mM Tris-HCl, 0.1 M NaCl, 0.25 mM EDTA, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), pH approx. 7.6.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	bovine
form	solution
packaging	pkg of 1 mL (20 mg/ml)
Торговая марка	Roche
pH	4.8-7.5
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

For standard applications.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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Bovine Serum Albumin Fraction V

Bovine Serum Albumin Fraction V is suitable for standard applications such as:

Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
Reducing agent
Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
Media supplement for media that require addition of protein
Carrier protein
Standard protein in gel electrophoresis and protein determination

Quality
Purity: 95% protein, 5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)
Preparation Note
Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	bovine
assay	98.5%
form	lyophilized
mol wt	M_r =68 kDa
packaging	pkg of 1 kg (10735108001)","pkg of 100 g (10735086001)","pkg of 50 g (10735078001)","pkg of 500 g (10735094001)
Торговая марка	Roche
application(s)	ELISA: suitable","Southern blotting: suitable","western blot: suitable
absorption	0.4 at 550 nm
UniProt accession no.	P02769,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	bovine ... ALB(280717)

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Bovine Serum Albumin Fraction V, fatty acid free 10775835001

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Bovine Serum Albumin, Fraction V, fatty acid free is used:

in lipid research to study lipid metabolism in biological systems
to identify transport mechanisms in living organisms
to study ligand binding
to investigate ionic binding
in the preparation of solutions during isolation of cardiomyocytes
as a blocking solution to incubate embryos for immunofluorescence
in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Features and Benefits

Free from fatty acids according to current quality control procedures (suitable for lipid research)
High quality
Low content of heavy metal ions
Long-term stability

Quality
Purity: 98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), 5% H2O,30 ppm heavy metals

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	bovine
assay	98.5% (electrophoresis)
form	lyophilized
mol wt	M_r 68 kDa
packaging	pkg of 50 g
Торговая марка	Roche
application(s)	immunofluorescence: suitable","ligand binding assay: suitable
UniProt accession no.	P02769,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	bovine ... ALB(280717)

For life science research only. Not for use in diagnostic procedures.
Loss on drying: ≤5.0%

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Bovine Serum Albumin Fraction V, heat shock

Quality Level	100,
biological source	bovine
assay	98.5%
form	lyophilized
mol wt	M_r =68,000
packaging	pkg of 1 kg (03116964001), pkg of 250 g (03116956001)
Торговая марка	Roche
solubility	0.40 (A₅₅₀)
absorption	0.40 at 550 nm
shipped in	wet ice
storage temp.	2-8°C

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Bovine Serum Albumin Fraction V, heat shock, fatty acid free 3117057001

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Quality
Purity: 98% albumin (electrophoresis), 98% protein
Contaminants: 1.2mg total fatty acids/g dry powder, 30ppm heavy metals, immunoglobulins are not detectable (by Ouchterlony)
Specifications
Moisture: 5%

Pharmacological assays; ion-binding, transport, or immunological studies
Fatty acid and lipid research
Stabilization of proteins and enzymes against proteolysis and denaturation

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	bovine
assay	98%
form	lyophilized
packaging	pkg of 100 g
Торговая марка	Roche
pH	~7.0
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Bovine Serum Albumin Fraction V, protease-free 3117332001

Bovine Serum Albumin Fraction V, protease-free is used:

for stabilization of purified enzymes
for site-blocking reagent in ELISA techniques
as a protein standard for determination of protein concentration

Quality
Contaminants: 0.001% heavy metals; proteases not detectable (casein digest)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	bovine
assay	≥=98.5% albumin basis (electrophoresis)
form	lyophilized
packaging	pkg of 50 g
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Buffers in a Box, Premixed PBS Buffer, 10x 11666789001

The premixed PBS (phosphate buffered saline) buffer is provided as a 10x solution.

Buffers in a Box, Premixed PBS Buffer, 10x has been used in immunohistochemistry and fluorescence in situ hybridization.
Quality
Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.

For life science research only. Not for use in diagnostic procedures.

form	solution (clear, colorless)
packaging	pkg of 4 L
Торговая марка	Roche
concentration	10 x
optimum pH	~7.0
shipped in	ambient
storage temp.	20-25°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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Buffers in a Box, Premixed SSC Buffer, 20x 11666681001

20x solution
Quality
Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.

For life science research only. Not for use in diagnostic procedures.

form	solution
packaging	pkg of 4 L
Торговая марка	Roche
pH	7.0
shipped in	ambient
storage temp.	20-25°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Buffers in a Box, Premixed TAE Buffer, 10x 11666690001

10x solution
Quality
Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.
Physical form
Appearance: clear, colorless solution

For life science research only. Not for use in diagnostic procedures.

form	solution
packaging	pkg of 4 L
Торговая марка	Roche
pH range	8.4
shipped in	ambient
storage temp.	20-25°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Buffers in a Box, Premixed TBE Buffer, 10x 11666703001

10x solution
Quality
Absence of contaminants: free of DNase and RNase according to the current Quality Control procedures.
Physical form
Solution, clear, colorless

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 4 L
Торговая марка	Roche
color	clear colorless
pH	~8.4
shipped in	ambient
storage temp.	20-25°C

pictograms	GHS08
signalword	Danger
hcodes	H360FD
pcodes	P201 - P202 - P280 - P308 + P313 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Calpain Inhibitor I 11086090001

Empirical Formula (Hill Notation):	C₂₀H₃₇N₃O₄
CAS Number:	110044-82-1
Molecular Weight:	383.53
Beilstein/REAXYS Number:	7656053
MDL number:	MFCD00065505
PubChem Substance ID:	329749147

Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I and calpain II.
Specificity
Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I (requiring 2–75 µM Ca2+ for activation) and calpain II (requiring 200–800 µM) with a Ki-value between 0.12 µM and 0.23 µM. Calpain II is inhibited to a lesser extent than calpain I. Calpain Inhibitor I is also a strong inhibitor of papain and cathepsin B and L. It shows only weak inhibition of cathepsin H and -chymotrypsin and does not inhibit trypsin. ID50 for 0.02 U platelet calpain is 0.05 µM.

Calpain inhibitor I inhibits calpain, which activates myosin light chain kinase and protein kinase C by partial proteolysis. The inhibitor should be membrane permeable due to low molecular weight and lack of charged residues.
Calpain Inhibitor I is used for western blotting methods.
Quality
Purity: 98% (from C); chromatographically homogeneous
Formula variant
C20H37N3O4
Preparation Note
Working concentration: 0.1 - 10 µM
The suggested starting concentration is 17 µg/ml. This is the concentration at which half maximal inhibition of calpain I is observed.
Working solution: Recommended solvent is DMF, ethanol, or methanol up to 10 mg/ml.
Storage conditions (working solution): -15 to -25 °C
Solutions in DMF, methanol, ethanol are stable for 4 weeks at -15 to -25 °C.
Reconstitution
Soluble in DMF, ethanol, or methanol to 10 mg/ml. For a stock solution, Roche recommends dissolving 1 mg of the inhibitor in 100 μl DMF, methanol, or ethanol. Before use, dilute with water or phosphate buffer (0.1 M, pH 7.5) to desired concentration. Solutions in DMF, ethanol, or methanol are stable for 2 to 3 days at 2 to 8 °C, and approximately 4 weeks at -15 to -25 °C. For best results, prepare solutions fresh before use.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	98% (chromatographically homogeneous)
form	powder
mol wt	M_r = 383.5
packaging	pkg of 25 mg
Торговая марка	Roche
mp	182 °C
solubility	DMF: 10 mg/mL, ethanol: 10 mg/mL, methanol: 10 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[H]C(=O)[C@H](CCCC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O
InChI	1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
InChI key	FMYKJLXRRQTBOR-BZSNNMDCSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Carboxypeptidase B, recombinant 8039852001

3.4.17.2 ( BRENDA | IUBMB )

Use Carboxypeptidase B for the sequence analysis of proteins by successive cleavage of basic amino acids from the C-terminus of proteins.
Peptidyl-L-lysine (-L-arginine) hydrolase
Biochem/physiol Actions
Carboxypeptidase B is a Zn2+-metalloprotease, catalyzing the hydrolysis of the basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides.
Quality
Contaminants: Contaminants expressed as percentage of carboxypeptidase B activity.
Carboxypeptidase A 2%
Trypsin (with Chymotrypsin as substrate) 0.005 U/mg

For life science research use only. Not for use in diagnostic precedures.

form	solution
Quality Level	100,
specific activity	~150 units/mg protein (at +25°C with hippuryl-L-arginine as the substrate)
shelf life	A decrease in activity of approx. 10% may occur within 6 months.
mol wt	34.6 kDa
packaging	pkg of 1 mL (2.8 mg)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
optimum pH	7.0-9.0
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash

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Carnitine Acetyltransferase 10103241001

2.3.1.7 ( BRENDA | IUBMB )

Carnitine acetyltransferase is a mitochondrial enzyme essential for fuel utilization. It is present in the mitochondrial matrix and peroxisome. It is involved in the fatty acid metabolism. It facilitates conversion of acetyl-CoA and carnitine to acetylcarnitine and CoA respectively. This conversion facilitates peroxisomal β-oxidation by importing acetyl group for mitochondrial oxidation.
Acetyl-CoA:carnitine O-acetyltransferase
Quality
Contaminants: <0.01% acetyl-CoA-deacylase ion in storage buffer.
Physical form
Suspension in 2.9 M ammonium sulfate solution, 50 mM K-phosphate, 1 mM EDTA, pH approximately 7.5

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~80 units/mg protein (At 25 °C with CoA and acetyl-D,L-carnitine as the substrates.)
packaging	pkg of 1 mL (5 mg)
Торговая марка	Roche
optimum pH	7.3-8.0
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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CAT ELISA 11363727001

Promoter activity in transfected eukaryotic cells is generally studied by linking the promoter sequence to a gene that encodes an easily detectable “reporter” protein, such as chloramphenicol acetyltransferase (CAT), -galactosidase (-Gal), or luciferase (Luc).
The bacterial enzyme chloramphenicol acetyltransferase (CAT, type I), encoded by Tn9 and having no eukaryotic equivalent, has become one of the standard markers used in transfection experiments. Traditionally, CAT activity is measured using a radioisotopic CAT assay.
The CAT ELISA is specific for CAT (type I) (Acetyl-CoA:chloramphenicol acetyltransferase, EC 2.3.1.28) from E. coli (Tn9-encoded).

For research use only. Not for use in diagnostic procedures.
The CAT ELISA is used to quantitatively measure CAT expression in eukaryotic cells that are transfected with a plasmid bearing a CAT-encoding reporter gene.
Features and Benefits
The CAT ELISA is specific for CAT (type I) (Acetyl-CoA:chloramphenicol acetyltransferase, EC 2.3.1.28) from E. coli (Tn9-encoded).

Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used
Faster: Approximately 4hours from start to finish
Safer: No radioisotopes are used
More accurate: Measures the actual amount of CAT protein synthesized, not just CAT enzyme activity
Sensitive: Equivalent to the sensitivity of the radioactive CAT assay
Easy to perform: Follows a standard ELISA protocol in microplates; allows Triton lysis of the transfected cells (CAT activity in the radioisotopic assay is rapidly inhibited by Triton)
More flexible: Allows the analysis of promoters in plant protoplasts (alternative method to the GUS assay) and animal cells

Packaging
1 kit containing 10 components.
Principle
The CAT ELISA is based on the sandwich ELISA principle. Following lysis of the transfected cells, the cell extracts (that contain CAT enzyme) are added to the wells of the microplate, which is precoated with a polyclonal antibody to CAT (anti-CAT). All CAT contained in the cell extracts binds to the anti-CAT antibody that is bound to the plate surface. Next, a digoxigenin-labeled antibody to CAT (anti-CAT-DIG) is added and binds to CAT. In the next step, an antibody to digoxigenin, conjugated to peroxidase (anti-DIG-POD), is added and binds to digoxigenin. In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate, yielding a colored reaction product. The absorbance of the sample is determined using an ELISA reader and is directly correlated to the level of CAT present in the cell extracts. The sensitivity of the assay can be enhanced using ABTS with the substrate enhancer.
Preparation Note
Working solution: CAT enzyme working dilution
Add 40 µl CAT enzyme stock solution (solution 1) to 3.96 ml Sample buffer (solution 7) to obtain a CAT enzyme working dilution (final concentration approx. 1 ng/ml), sufficient to produce a calibration curve in duplicate.
Handling instructions

The CAT enzyme standard dilutions should be prepared freshly before use and should not be stored.
Prepare the standard dilution series in reaction tubes in 1:2 dilution steps as described in the table below.
To obtain a calibration curve, we recommend using the five concentrations listed.
200 µl of each dilution is needed per well.
To ensure that the measurements and the calibration curve are accurate, we recommend preparing two samples of each concentration for measurement.
To avoid carryover of the higher conc. solution to the lower conc. samples, use a fresh pipette tip for each dilution step.
Each dilution must be measured in duplicate.

Storage conditions (working solution): Sample Buffer 2 to 8 °C
Stability of ready-to-use sample buffer: 2 months at 2 to 8 °C, otherwise aliquoted at - 15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 192 tests
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H317 - H319 - H340 - H350 - H412
pcodes	P201 - P261 - P273 - P280 - P308 + P313 - P333 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Cell Death Detection ELISA 11544675001

The Cell Death Detection ELISA serves as photometric enzyme immunoassay for the qualitative and quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death. This assay is based on the quantitative sandwich-enzyme immunoassay-principle using mouse monoclonal antibodies directed against DNA and histones, respectively. The antibodies used are not species specific.
Specificity
Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, and Xenopus). Anti-DNA-POD antibody binds to ss- and dsDNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.

Cell Death Detection ELISA has been used to measure Caspase-3 activity, intracellular reactive oxygen species (ROS) and cell apoptosis.
For research use only. Not for use in diagnostic procedures.
Specific determination of mono- and oligonucleosomes in the cytoplasmic fraction of cell lysates.
Packaging
1 kit containing 9 components.
Preparation Note
Working solution: Coating solution
Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer.
Washing solution
Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly.
Sample solution
Preparation of the sample solution depends on the cell system used and the extent of cell death.
Example: Dilute 25 µl of sample in 225 µl Incubation buffer.
Conjugate solution
Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer.
Substrate solution
Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer.
Allow to come to 15 to 25 °C before use.
Note: The ABTS solution is light sensitive over extended periods of time.
Storage conditions (working solution): Anti-histone
2 to 8 °C for 2 months
Anti-DNA-POD
2 to 8 °C for 2 months
Coating solution
Prepare immediately before use!
Washing solution
2 to 8 °C for 2 months
Sample solution
2 to 8 °C for 2 months
Conjugate solution
Prepare immediately before use!
Substrate solution
2 to 8 °C for 1 month, protected from light.
Reconstitution
Anti-histone
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly.
Anti-DNA-POD
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 96 tests
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317 - H319
pcodes	P261 - P280 - P333 + P313 - P337 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Cell Proliferation ELISA, BrdU (chemiluminescent) 11669915001

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Specificity
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:

for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:

Detection and quantification of cell proliferation induced by growth factors and cytokines
Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Packaging
1 kit containing 7 components.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤1,000 tests
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS07
signalword	Danger
hcodes	H225 - H317 - H319
pcodes	P210 - P233 - P261 - P280 - P303 + P361 + P353 - P370 + P378
RIDADR	UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany	WGK 2

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Cell Proliferation ELISA, BrdU (colorimetric) 11647229001

Colorimetric immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Specificity
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

For research use only. Not for use in diagnostic procedures.
The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:

Detection and quantification of cell proliferation induced by growth factors and cytokines
Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Packaging
1 kit containing 6 components.
Preparation Note
Working concentration: The kit antibody (Anti-BrdU-peroxidase) has, after reconstitution, a concentration of 7.5 U/ml, after dilution 0.075 U/ml. Instead of the kit antibody, you can also use the Anti-BrdU-Antibody, Fab fragments.This antibody is double-concentrated, so you have to dilute it after reconstitution in 1 ml with 1:200.
Working solution: BrdU labeling solution

Dilute BrdU labeling reagent 1:100 with sterile culture medium (resulting concentration: 100 µM BrdU).
For one 96-well MP, 1 ml BrdU labeling solution is required if the cells were cultured in 100 µl /well (10 µl/well) and 2 ml BrdU labeling solution is required if the cells were cultured in 200 µl/well (20 µl/well).
Anti-BrdU-peroxidase stock solution

Dissolve Anti-BrdU-peroxidase in 1.1 ml double-dist. water for 10 minutes and mix thoroughly.
Anti-BrdU-peroxidase working solution

Dilute Anti-BrdU-peroxidase stock solution 1:100 with antibody dilution solution. For one 96-well MP dilute 100 µl Anti-BrdU-peroxidase stock solution in 10 ml antibody dilution solution
Washing solution

Dilute Washing buffer concentrate 1:10 with double-dist. water.
For one 96-well MP dilute 10 ml Washing buffer concentrate with 90 ml double-dist. water.
Storage conditions (working solution): BrdU labeling solution
The undiluted BrdU labeling reagent (1000x): At 2 to 8 °C for several months protected from light.
The diluted BrdU labeling reagent: At 2 to 8 °C stable for several weeks. Store protected from light. For long-term storage it is recommended to store the BrdU labeling solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase stock solution
At 2 to 8 °C for several months. For long-term storage it is recommended to store the solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase working solution
Prepare shortly before use. Do not store.
Washing solution
At 2 to 8 °C for several weeks.
Reconstitution
The working solution for the antibody should be phosphate buffered saline containing 1% BSA, pH 7.4

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤1,000 tests
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS07
signalword	Danger
hcodes	H225 - H317 - H319
pcodes	P210 - P233 - P261 - P280 - P303 + P361 + P353 - P370 + P378
RIDADR	UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany	WGK 2

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Cell Proliferation Kit I (MTT) 11465007001

The Cell Proliferation Kit I (MTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
MTT was the first tetrazolium salt described. It is cleaved to formazan by enzymes of the endoplasmic reticulum. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Colorimetric assay (MTT based) for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity.

The Cell Proliferation Kit I (MTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:

Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients
Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
Assessment of growth-inhibitory antibodies and physiological mediators
Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Features and Benefits

Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
Accurate: The absorbance obtained strongly correlates to the cell number.
Sensitive: Detect low cell numbers.
Fast: Process a large number of samples using a multi-well ELISA reader.

The assay is based on the cleavage of the tetrazolium salt MTT in the presence of an electron-coupling reagent. The water-insoluble formazan salt produced must be solubilized in an additional step. Cells grown in a 96-well tissue culture plate are incubated with the MTT solution for approximately 4 hours. After this incubation period, a water-insoluble formazan dye is formed. After solubilization, the formazan dye is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells (see below).
Packaging
1 kit containing 2 components.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
After thawing, the MTT labeling reagents may be stored protected from light at 2 to 8 °C for up to 4 weeks, in which case reagent filtration through a 0.2 µm pore size membrane is recommended.

For life science research only. Not for use in diagnostic procedures.

form	liquid
Quality Level	100,
usage	sufficient for 2,500 tests
packaging	pkg of 1 kit
Торговая марка	Roche
storage condition	protect from light
application(s)	cell analysis: suitable, detection: suitable, tissue culture: suitable
_max	550-600 nm
detection method	colorimetric
shipped in	dry ice
storage temp.	20°C

pictograms	GHS05
signalword	Danger
hcodes	H318
pcodes	P280 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Cell Proliferation Kit II (XTT) 11465015001

The Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
More recently, the tetrazolium salt XTT was described. In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required. The tetrazolium salt XTT is cleaved to formazan by a complex cellular mechanism. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.

The Cell Proliferation Kit II (XTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:

Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds.
Assessment of growth-inhibitory antibodies and physiological mediators that inhibit cell growth.
Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth.
cell viability assay.

Features and Benefits

Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
Accurate: The absorbance obtained strongly correlates to the cell number.
Sensitive: Detect low cell numbers.
Fast: Process a large number of samples using a multi-well ELISA reader.

Packaging
1 kit containing 2 components.
Principle
The assay is based on the cleavage of the tetrazolium salt XTT in the presence of an electron-coupling reagent, producing a soluble formazan salt. This conversion only occurs in viable cells. Cells grown in a 96-well tissue culture plate are incubated with the XTT labeling mixture for 2 - 20 hours. After this incubation period, the formazan dye formed is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
Preparation Note
Working solution: Preparation of solutions
Thaw XTT labeling reagent and electron-coupling reagent, respectively in a water bath at 37 °C. Mix each vial thoroughly to obtain a clear solution.
XTT labeling mixture
To perform a cell proliferation assay (XTT) with one microplate (96 wells) mix 5 ml XTT labeling reagent with 0.1 ml electron coupling reagent.
Note: To obtain reliable results thaw and mix XTT labeling reagent and electron coupling reagent immediately before use.
Working instruction
Cells are grown in microplates (tissue culture grade, 96 wells, flat bottom) in a final volume of 100 µl culture medium per well, according to the media needs of the cells in a humidified atmosphere (e.g., 37 °C, 6.5% CO2).
The incubation period of the cell cultures depends on the particular experimental approach and on the cell line, used for the assay. For most experimental setups, the incubation of cells for 24 to 96 hours is appropriate.
After the incubation period, add to each well 50 µl of the XTT labeling mixture, prepared as described above (final XTT concentration 0.3 mg/ml).
Incubate the microplate for 4 to 24 hours in a humidified atmosphere (e.g., 37 °C, 6.5% CO2).
Note: The incubation time varies with the individual experimental setup (e.g., cell type and cell concentration, used). Therefore, we recommend to measure the absorption as described at different time points after addition of XTT labeling mixture (e.g., 4, 6, 8, 12, and 18 hours) using one and the same microplate to determine the optimal incubation period for the particular experimental setup.
Storage conditions (working solution): Thaw reagents immediately before use. It is recommended to prepare appropriate aliquots [5 ml XTT labeling reagent and 0.1 ml electron coupling reagent are required for the performance of the assay with one microplate (96 wells)]
Note: Avoid repeated thawing and freezing.

For life science research only. Not for use in diagnostic procedures.

form	liquid
Quality Level	100,
usage	sufficient for 2,500 tests
packaging	pkg of 1 kit
Торговая марка	Roche
storage condition	protect from light
application(s)	cell analysis: suitable, detection: suitable, tissue culture: suitable
_max	450-500 nm
detection method	colorimetric
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

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Cell Viability Imaging Kit 5x96 6432379001

The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.
The kit is designed for 5 x 96 reactions for use in medium to high throughput analysis of cell behavior.

Determine total cell number using fluorescence staining of cell nuclei.
Fluorimetrically determine the percentage of dead cells based on membrane permeability.
Verify the labeling of viable cells quantified by fluorescence staining using a vital dye

Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.
Features and Benefits
Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:

No risk of losing cells in the one step assay: There are no fixation or washing steps.
Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
Obtain highly reproducible results using automated imaging and data evaluation with Roches Cellavista System.

Packaging
1 kit containing 3 components.
Preparation Note
Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.
Storage and Stability
Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 5 96-well plate(s)
packaging	pkg of 1 kit
Торговая марка	Roche
storage condition	protect from light
fluorescence	_ex 361 nm; _em 486 nm (nuclei dye), _ex 490 nm; _em 515 nm (viable dye), _ex 535 nm; _em 617 nm (dead dye)
detection method	fluorometric
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

pictograms	GHS08
signalword	Warning
hcodes	H341
pcodes	P201 - P202 - P280 - P308 + P313 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Cellular DNA Fragmentation ELISA 11585045001

The Cellular DNA Fragmentation ELISA is a photometric enzyme-linked immunosorbent assay (ELISA) for the detection of 5-bromo-2-deoxy-uridine (BrdU)-labeled DNA fragments in culture supernatants and cell lysates. BrdU is used as a metabolic labeling agent by the nuclear DNA of target cells. This BrdU-labeled DNA can be detected easily and quantified using a monoclonal antibody against BrdU in an ELISA. This kit is a non-radioactive alternative to the [3H]-thymidine release assay, the [51Cr]-release assay, and the [3H]-thymidine DNA fragmentation assay.

For research use only. Not for use in diagnostic procedures.
The Cellular DNA Fragmentation ELISA is for the quantification of BrdU-labeled DNA fragments either released from cells during necrosis or cell-mediated cytotoxicity, or within the cytoplasm of apoptotic cells.
Features and Benefits

Nonradioactive assay system
Sensitive (1 x 103 cells/well)
Low assay background
No additional dilution steps required
No washing of the cells required
Results obtained correlate to those obtained by standard methods
Denaturation/fixation of DNA in the microplate by microwave irradiation
Fast (4.5 - 5.5 hours)

Packaging
1 kit containing 8 components.
Preparation Note
Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Storage conditions (working solution): Storage of coated plates: 1 week at 2 to 8 °C.
The anti-BrdU-POD stock solution is stable at 2 to 8 °C for several months; for long- term storage it is recommended to store the solution in aliquots at -15 to -25 °C;
The anti-BrdU-working solution should not be stored.
For the anti-DNA-clone MCA-33 we do no have stability data. However it should be no problem to aliquot and store this non-conjugated antibody at -15 to -25 °C for longer storage.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤500 tests
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

3-[(3-Cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate

pictograms	GHS07
signalword	Warning
hcodes	H317 - H319 - H412
pcodes	P261 - P273 - P280 - P333 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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CHAPS

Empirical Formula (Hill Notation):	C₃₂H₅₈N₂O₇S · xH₂O
CAS Number:	75621-03-3
Molecular Weight:	614.88 (anhydrous basis)

Solubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.
Physical form
Crystals
Preparation Note
Working concentration: PAGE: 6.5 to 13 mM
Storage conditions (working solution): Do not store in solution, because precipitation may occur.

For life science research only. Not for use in diagnostic procedures.

assay	98% (from N)
mol wt	614.88 g/mol (anhydrous basis)
packaging	pkg of 10 g (10810118001), pkg of 50 g (10810126001)
Торговая марка	Roche
application(s)	dialysis: suitable
CMC	4 mM
mp	157 °C (dec.) (lit.)
solubility	>50% (Aqueous solutions)
absorption	<0.06% at 278 nm in water at 1 % (w/v) (Ease of removal: ++ Note:* * Arbitrary scale ranges from ++ to )
shipped in	wet ice
storage temp.	2-8°C
SMILES string	O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI	1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key	SJCUTFKCLFLIFE-JWTJKVBLSA-N

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Chromozym PK 10378445001

Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
Quality
Contaminant: <0.5% free 4-nitraniline
Sequence
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
Principle
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Chromozym is a registered trademark of Pentapharm AG, Basel

description	Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
Quality Level	100,
assay	90% (enzymatic)
form	powder
mol wt	M_r 702.9
packaging	pkg of 20 mg
Торговая марка	Roche
shipped in	ambient
storage temp.	room temp

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Chromozym PL 10378461001

Chromozym® PL is a chromogenic substrate. It is a synthetic tripeptide substrate and is more plasmin-specific.

Chromozym® PL has been used:

to determine the plasmin enzymatic activity using human umbilical vein endothelial cells (HUVEC) cell extracts
as a chromogenic substrate for proteolytic activity in murine lung homogenates
as a substrate of plasmin in plasminogen activation assays

Quality
Purity: 90% Tosyl-Gly-Pro-Lys-4-nitranilide acetate (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Formula: C26H35N6O7SCOOCH3
Sequence
Tosyl-glycyl-polyl-lysine-4-nitranilide-acetate
Physical form
Powder
Preparation Note
Working concentration: Approximately 0.3 to 0.6 mM
Storage conditions (working solution): 2 to 8 °C
A substrate solution (3 mM) is stable for at least two weeks if stored at 2 to 8 °C. Avoid contamination with germs.

For life science research only. Not for use in diagnostic procedures.

Chromozym is a registered trademark of Pentapharm AG, Basel

assay	90%
Quality Level	100,
form	powder
packaging	pkg of 20 mg
Торговая марка	Roche
shipped in	ambient
storage temp.	room temp

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Chromozym t-PA 11093037001

N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide acetate

Use Chromozym® t-PA (tissue plasminogen activator) (C24H32N8O7S x CH3COOH) as a substrate for the determination of t-PA, both in purified preparations and in cell culture supernatants. Moreover, it can be applied to evaluate the content of one-chain and two-chain t-PA.
Quality
Purity: >90% (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Sequence
N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide-acetate
Preparation Note
Working concentration: Approximately 0.25 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym t-PA (4mM) is stable for at least two weeks if stored at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Chromozym is a registered trademark of Pentapharm AG, Basel

Quality Level	100,
assay	90% (enzymatic)
form	powder
mol wt	636.7
packaging	pkg of 20 mg
Торговая марка	Roche
concentration	0.25 mM
shipped in	wet ice
storage temp.	20-25°C

Chromozym® TH is a chromophoric substrate mostly used to detect thrombin activity†
Tosyl-Gly-Pro-Arg-4-nitranilide acetate

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Chromozym TH

Use Chromozym® TH as a substrate for the determination of serine proteases, specifically thrombin in aqueous solutions.
Quality
Contaminant: 1% free 4-nitraniline
Sequence
Tosyl-glycyl-polyl-arginine-4-nitranilideacetate
Preparation Note
Working concentration: Approximately 0.2 mM
Storage conditions (working solution): 2 to 8 °C
A substrate solution (approx. 2 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Chromozym is a registered trademark of Pentapharm AG, Basel

assay	90% (Tosyl-Gly-Pro-Arg-4-nitranilide acetate (enzymatic)), 90% (enzymatic)
Quality Level	100,
form	powder
mol wt	M_r 662.62
packaging	pkg of 100 mg (11585398001), pkg of 20 mg (10206849001)
Торговая марка	Roche
shipped in	ambient
storage temp.	20-25°C

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Chymostatin 11004638001

CAS Number:	9076-44-2
MDL number:	MFCD00071059
PubChem Substance ID:	329749270

Chymostatin is a mixture of three components, A, B, and C. The component A being N-[((S)-1-carboxy-2-phenylethyl)-carbamoyl]--[2-iminohexahydro-4(S)-pyrimidyl]-L-glycyl-L-leucyl-phenylalaninal. The other two components B and C differ in that the L-leucyl residue is substituted by L-valine and L-isoleucine, respectively.

Chymostatin is a specific inhibitor of α-, β-, γ-, and δ-chymotrypsin.
Biochem/physiol Actions
Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts.
Quality
Performance tested.
Formula variant
C31H41N7O6
Preparation Note
Working concentration: 6 to 60 µg/ml (10 to 100 µM)
1 U chymotrypsin is inhibited to 49% of the original activity by 1.8 µg of chymostatin.
Thin-layer chromatography: butanol/methanol/H2O = 4 / 1 / 2
Working solution: Soluble in glacial acetic acid or DMSO to 20 mg/ml. Sparingly soluble in water, methanol, or ethanol. Insoluble in ethyl acetate, petroleum and ethyl ethers, hexane, or chloroform (CHCl3).
It is recommended to dissolve the inhibitor in 1% acetic acid in higher concentration and to adjust the concentration wanted with phosphate buffer, 0.05 M, pH 7.0, which is common for chymotrypsin assay.
CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc.
Storage conditions (working solution): -15 to -25 °C
Dilute solutions should be stored frozen in aliquots at -15 to -25 °C and are stable for approximately one month. Avoid repeated freezing. Growth of microorganisms should be avoided as proteases from microbial origin may hydrolyze the peptides.
Solubility testing in glacial acetic acid at 10 mg/ml yields a clear solution, which is usually colorless, but can be yellow in appearance. It is reportedly also soluble in DMSO; only slightly soluble in water and short-chain alcohols; insoluble in ethyl acetate, butyl acetate, ether, hexane, and petroleum ether. Stock solutions (10 mM) can be prepared in DMSO and are stable for months at -20 °C. Stock solutions can also be made in 0.1 M HCl. Dilute solutions (10-100 μM) are only stable for several hours, due to oxidation of the terminal aldehyde.

For life science research only. Not for use in diagnostic procedures.

form	powder
Quality Level	100,
mol wt	M_r = 607.71
packaging	pkg of 10 mg
Торговая марка	Roche
mp	205 °C
solubility	acetic acid: soluble 20 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OC(C(NC(NC(C1NC(NCC1)=N)C([F,Cl,Br,I]C)=O)=O)CC2=CC=CC=C2)=O
InChI key	MRXDGVXSWIXTQL-HYHFHBMOSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Chymotrypsin Sequencing Grade 11418467001

3.4.21.1 ( BRENDA | IUBMB )

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.
Specificity
Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.
Quality
Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.
Preparation Note
Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, 2-macroglobulin, 1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.
Reconstitution
Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	90%
form	lyophilized (salt-free)
specific activity	70 units/mg protein (at 25 °C, with ATEE as a substrate)
mol wt	M_r 25 kDa
packaging	pkg of 4 x 25 µg
Торговая марка	Roche
optimum pH	7.0-9.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Citrate Lyase (CL) 10354074001

4.1.3.6 ( BRENDA | IUBMB )

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

It was used in enzymatic assay to measure citrate concentration using microtiter plates.
Quality
Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each
Physical form
Lyophilizate, stabilized with BSA, sucrose, MgSO4 and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	0.25 U/mg (At 25 °C with citrate as the substrate.)
packaging	pkg of 120 U
Торговая марка	Roche
optimum pH	8.0-9.0
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Colcemid 10295892001

Empirical Formula (Hill Notation):	C₂₁H₂₅NO₅
CAS Number:	477-30-5
Molecular Weight:	371.43
Beilstein/REAXYS Number:	2822892
MDL number:	MFCD00075459
PubChem Substance ID:	329749009

Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.
Biochem/physiol Actions
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Physical form
Solution (10 μg/ml), filtered through 0.2 μm pore size membrane.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	sterile; 0.2 µm filtered
form	solution
packaging	pkg of 20 mL (10 µg/ml)
Торговая марка	Roche
application(s)	blocking: suitable
impurities	microbial, tested
solubility	water: miscible
shipped in	wet ice
storage temp.	2-8°C
SMILES string	CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
InChI	1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
InChI key	NNJPGOLRFBJNIW-HNNXBMFYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

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Collagen 11179179001

Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.
Specificity
Active on most mammalian cells.

Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.
Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.
Biochem/physiol Actions
Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.
Features and Benefits
Contents
Lyophilizate, cell culture grade, 3 vials of 10mg
Specifications
Biological activity: Tested for the promotion of adherence of HUV-EC cells.
Preparation Note
Working concentration: 5 µg/cm2
5 µg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
Reconstitution
IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.

For life science research only. Not for use in diagnostic procedures.

biological source	rat tail
Quality Level	100
sterility	sterile
form	lyophilized (clear, colorless solution after reconstitution)
packaging	pkg of 30 mg
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	P02454,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	rat ... Col1a1(29393)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Collagenase B

3.4.24.3 ( BRENDA | IUBMB )

Collagenase B is prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization. Collagenase from Clostridium histolyticum is a collagenolytic enzyme. It interacts with collagen-like substances and is responsible for the degradation of collagen into small peptides. It is widely used for the disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors) and for the preparation of single-cell suspensions for the establishment of primary cell culture systems. Collagenase B is recommended when yield and viability are important.
Specificity
Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.

Clostridium collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc. However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically.
Collagenase B has been used for preparation of cells from mouse aortas and colorectal cancer tissue.
Specifications
Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wunsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase B has a normal to high collagenase activity and a higher than average clostripain activity (usually >10U/mg).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean), serum
Unit Definition
Collagenase from Roche is assayed in Wnsch units (1 µmol of product formed per minute at +25 °C with Wnsch substrate).
Frequently, collagenase activities are given in Mandl units (1 µmol leucine liberated from collagen in 5 hours at +37 °C).
Unfortunately, there is no consistent conversion factor between the two units of activity, since the Mandl unit depends, in part, on the concentration of contaminating proteases in the collagenase preparation, an indefinable variable. A purer collagenase preparation would actually give a lower specific activity in Mandl units than a crude preparation. Clostridium preparations typically give conversion factors of approximately 1:1800 (e.g., a particular lot of Clostridium collagenase contained approximately 0.15 Wnsch U/mg and 250 Mandl U/mg).
Preparation Note
Activator: Ca2+
Working concentration: 0.5 to 2.5 mg/ml
Storage conditions (working solution): -15 to -25 °C
Roche recommends reconstituting only the amount of lyophilizate needed for immediate use. The reconstituted solution can be stored at -15 to -25 °C for up to one week. Avoid repeated freezing and thawing since activity decreases after reconstitution.
Reconstitution
Reconstitution in any balanced salt solution (e.g., HBSS)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	non-sterile
form	lyophilized
packaging	pkg of 100 mg (11088807001), pkg of 2.5 g (11088831001), pkg of 500 mg (11088815001)
Торговая марка	Roche
concentration	0.5-2.5 mg/mL
optimum pH	6.0-8.0
shipped in	wet ice
storage temp.	2-8°C