Molecular Biology &amp; Functional Genomics

Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.
Ribonucleoside vanadyl complexes has been used:

as a component of lysis buffer for cell lines prior to Poly(U) pull down assay
to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment
as a component of hybridization buffer for human retinal slices

Biochem/physiol Actions
Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.

Quality Level	200,
concentration	200 mM
shipped in	dry ice
storage temp.	−20°C

RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.

pictograms	GHS07
signalword	Warning
hcodes	H302 - H412
pcodes	P264 - P270 - P273 - P301 + P312 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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RNAzol® RT

Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.
Features and Benefits

Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
High yields and increased quality of isolated RNA
Completed at room temperature in less than an hour starting with fresh tissue or cells

RNAZol is a registered trademark of Molecular Research Center, Inc.

Quality Level	100,
usage	1 mL sufficient for 10⁷ cells, 1 mL sufficient for 100 mg tissue (or)

SYBR® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling.
Features and Benefits

pictograms	GHS05,GHS07,GHS08,GHS09
signalword	Danger
hcodes	H302 + H312 + H332 - H314 - H341 - H373 - H411
pcodes	P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
RIDADR	UN 1760 8 / PGII
WGK Germany	WGK 3
Flash Point F	230.0 °F
Flash Point C	110 °C

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SYBR® Green JumpStart™ Taq ReadyMix™

MDL number:	MFCD03458666
NACRES:	NA.55

This master mix allows consistency from one reaction to the next
Designed to minimize contamination
Reduced primer dimers
Reduced set-up time as compared to manual or wax HotStart® methods
Allows for room temperature set-up and contains a fluorescent dye; Ideal for qPCR applications

Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield.
SYBR Green JumpStart Taq ReadyMix for SYBR based QPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments.

Packaging
Default reaction volume is 50 µL
20RXN is packaged as 1 X 500 µL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

SYBR Green JumpStart Taq ReadyMix combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2x concentrate for ease of use. Simply add 25 µL of the 2x mix to DNA template, primers and water. The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.
Principle
SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigmas SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Quality Level	200,
form	liquid
feature	hotstart
concentration	1.25 units/reaction (50 µL reaction volume)
application(s)	qPCR: suitable
Featured Industry	Agriculture
compatibility	for use with ABI 5700
detection method	SYBR^® Green
shipped in	wet ice
storage temp.	20°C

SYBR® Green JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains SYBR® Green I, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable

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SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR).
Features and Benefits

The master mix allows consistency and reproducibility from one reaction to the next
Reduced preparation time and the risk of contamination from multiple pipetting steps
Reduced set-up time as compared to manual or wax Hot Start methods
Increased specificity and target yield
Reduced primer dimers
Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application

SYBR Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.

Packaging
Default reaction volume is 50 µL
20RXN is packaged as 1 X 500 µL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Quality Level	200,
form	liquid
feature	hotstart
concentration	1.25 units/reaction (50 µL reaction volume)
application(s)	qPCR: suitable
color	colorless
compatibility	for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus
detection method	SYBR^® Green
shipped in	wet ice
storage temp.	20°C

For SYBR based capillary qPCR, optimized for use on Roche Lightcycler
Features and Benefits

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

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SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation

Convenient 2x concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler and is ideal for high throughput applications
Increased specificity & target yield - JumpStart Taq polymerase prevents non-specific product resulting in more accurate CT values and improved quantitation

Packaging
Default reaction volume is 20 µL
100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL
A tube of 25 mM MgCl2 is provided for easy optimization of the QPCR reaction.

SYBR Green JumpStart Taq ReadyMix, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2x concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.
SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.
Principle
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigmas SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.
To prepare a reaction, 10 µL of ReadyMix is added to primers, template and water for a final reaction volume of 20 µL.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LightCycler is a registered trademark of Roche
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Quality Level	200,
form	liquid
feature	hotstart
concentration	1 units/reaction (20 µL reaction volume)
application(s)	qPCR: suitable
color	colorless
compatibility	for use with Roche LightCycler 480
detection method	SYBR^® Green
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable

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SYBR® Green Quantitative RT-qPCR Kit QR0100-1KT

NACRES:

NA.55

The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

SYBR® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay to detect specific genetic clusters of genogroup I and II noroviruses, for chikungunya viral (CHIKV) RNA quantification, and to detect mRNA expression levels.
The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression. Sigma′s QRT-PCR ReadyMix combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix.
Features and Benefits

The master mix allows consistency and reproducibility from one reaction to the next
Reduced preparation time and the risk of contamination from multiple pipetting steps
Reduced set-up time as compared to manual or wax Hot Start methods
JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
Broad instrument compatibility
Includes a separate ROX reference dye vial for reaction normalization

Packaging
1 kit sufficient for 100 reactions at 50 μL each

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Quality Level	200,
feature	hotstart
application(s)	RT-qPCR: suitable
color	colorless
detection method	SYBR^® Green
shipped in	wet ice
storage temp.	20°C

T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 µg/100 µl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
Specificity
T4 gene 32 protein is a single-strand specific, helix-destabilizing protein encoded by gene 32 of the phage T4 genome. It is required for the replication of DNA and genetic recombination in Escherichia coli cells that are infected with T4 bacteriophage.

pictograms	GHS05,GHS07,GHS09
signalword	Danger
hcodes	H314 - H317 - H410
pcodes	P261 - P272 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
RIDADR	UN 3316 9
Flash Point F	Not applicable
Flash Point C	Not applicable

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T4 Gene 32 Protein

T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:

Optimization of PCR (See "Product Description" below.)
Stimulation of in vitro DNA synthesis
Stabilization of single-stranded regions of DNA or RNA
Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
Helping restriction enzyme digestions go to completion
q-PCR

Quality
Absence of contaminants
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:

nonspecific exo- and endonucleases
single-strand DNases (nicking activities)
single-strand-specific exonucleases
RNases

Physical form
Protein solution, 1 - 10mg/ml, in storage buffer (20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 0.5mM DTT, 50% glycerol [v/v], pH approximately 8.0)
Preparation Note
Working concentration: 1 to 10 mg/ml

For life science research only. Not for use in diagnostic procedures.

biological source	Escherichia coli (infected with phage T4amN134/amBL292/amE218)
Quality Level	100,
form	solution
packaging	pkg of 100 µg (10972983001), pkg of 500 µg (10972991001)
Торговая марка	Roche
optimum pH	~8.0
shipped in	dry ice
storage temp.	20°C

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Taq DNA Polymerase (1 U/l), dNTPack

2.7.7.7 ( BRENDA | IUBMB )

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.

Taq DNA Polymerase (1 U/µl), dNTPack may be used for:

PCR
RT-PCR
Other primer-extension reactions, such as sequencing and labeling

Packaging
1 kit containing 5 components
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/µl

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤2,000 reactions (04738241001), sufficient for ≤500 reactions (04738225001)
Quality Level	100,
feature	dNTPs included, hotstart: no
packaging	pkg of 1,000 U (04738241001 [4 x 250 U]), pkg of 250 U (04738225001)
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
optimum pH	~9.0 (20 °C)
shipped in	dry ice
storage temp.	−20°C

Taq DNA Polymerase from Thermus aquaticus has been used:

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Taq DNA Polymerase from Thermus aquaticus

CAS Number:	9012-90-2
Enzyme Commission (EC) Number:	2.7.7.7 ( BRENDA \| IUBMB )
MDL number:	MFCD00166026
NACRES:	NA.55

in the quantification of fungal growth by PCR (polymerase chain reaction) and photometric assay
in conventional RT(reverse transcriptase)-PCR
in SSR (simple sequence repeats) genotyping

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Taq DNA Polymerase with 10x reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10x reaction buffer including MgCl2 (D1806) or a 10x reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Low per unit cost of Taq

Packaging

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 53 polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Quality Level	200
recombinant	expressed in E. coli
form	liquid
mol wt	94 kDa
feature	dNTPs included: no, hotstart: no
concentration	5 units/µL
application(s)	PCR: suitable
color	colorless
suitability	suitable for PCR and automated sequencing reactions
Featured Industry	Agriculture
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1

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Taq DNA Polymerase, dNTPack

2.7.7.7 ( BRENDA | IUBMB )

Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.
Taq DNA Polymerase is a highly processive 53 DNA polymerase that lacks 35 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Contents
Taq DNA Polymerase, 5 U/µl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.

Achieve the most consistent results in simple, routine PCR by using Roche Applied Science Taq DNA Polymerase, which is held to our rigorous purity and quality standards. The Taq DNA Polymerase, dNTPack, combines our standard preparation (5 U/µl) of recombinant Taq DNA Polymerase with our convenient, ready-to-use PCR Nucleotide Mix. In all other respects, this preparation is identical to our standard preparation and can be used for:

PCR
RT-PCR
Other primer-extension reactions, such as sequencing and labeling

Packaging
1 kit containing 3 components
Quality
Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/µl

Reliable reproducible results: High lot-to-lot consistency.
No need to test each lot: Taq DNA Polymerase is rigorously tested.
Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

For life science research only. Not for use in diagnostic procedures.
Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.

Quality Level	100,
description	Number of Reactions: If 1.25 U are used per 50 µL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage.
usage	sufficient for 2,000 reactions (04728904001), sufficient for 4,000 reactions (04728858001), sufficient for 400 reactions (04728874001), sufficient for 80 reactions (04728866001), sufficient for 800 reactions (04728882001)
feature	dNTPs included, hotstart: no
packaging	pkg of 1,000 U (04728882001 [4 x 250 U]), pkg of 2,500 U (04728904001 [10 x 250 U]), pkg of 5,000 U (04728858001 [20 x 250 U]), pkg of 100 U (04728866001), pkg of 500 U (04728874001 [2 x 250 U])
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
optimum pH	~9.0 (20 °C)
shipped in	dry ice
storage temp.	20°C

ThermaGo™ are modified nucleic acids for error free PCR amplification, which improves PCR specificity, suppresses mis-priming and prevents non-specific PCR products.

ThermaGo™ interacts with DNA polymerase during PCR in the annealing and extension steps to increase specificity and inhibit non-specific amplification.

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ThermaGo™

ThermaStop™ and ThermaGo™ act together during PCR amplification at different temperatures to increase specificity and prevent errors.
Reconstitution
Dissolve in Tris pH 8.3 buffer

ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.

Quality Level	100,
assay	(Dual, HPLC)
form	lyophilized powder
packaging	pkg of One unit of ThermaGo^™ is defined as the amount required for maximum improvements in amplification reactions containing 1 unit of Taq DNA polymerase in a volume of 25 µl. A
scale	500 and 2500 (units)
application(s)	PCR: suitable
color	opaque
shipped in	ambient
storage temp.	2-8°C

ThermaStop™ is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop™ by cooling the reaction. Adding ThermaStop™ to your PCR reactions improves sensitivity and total product yield.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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ThermaStop™

Reconstitution
Dissolve in Tris pH 8.3 buffer

For PCR that requires reduced non-specific amplification, including for downstream applications with specific amplification products for NGS and Gibson assembly.
For highly multiplex PCR
For prevention of primer dimer formation

ThermaStop is a trademark of ThermaGenix Inc.

assay	(Dual, HPLC)
form	lyophilized powder
packaging	pkg of One unit of ThermaStop^™ is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 µl.
scale	500 and 2500 (units)
application(s)	PCR: suitable
color	opaque
shipped in	ambient
storage temp.	2-8°C

ThermaStop™-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcriptase-PCR assays containing gene-specific primers. ThermaStop™-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors. ThermaStop™-RT improves detection sensitivity and specific product yield by eliminating priming errors at low temperature.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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ThermaStop™-RT

RT-PCR, *Please note ThermaStop™-RT should not be used in combination with ThermaStop™ or ThermaGo™.
Reconstitution
Dissolve in Tris pH 8.3 buffer

ThermaGo is a trademark of ThermaGenix Inc.
ThermaStop is a trademark of ThermaGenix Inc.

assay	(Dual, HPLC)
form	lyophilized powder
packaging	pkg of One unit of ThermaStop^™-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase (RT) and one unit of hot-start Taq Polymerase in a volume of 20 ?L and a temperature of 50 degrees C.
scale	250 and 1250 (units)
application(s)	RT-PCR: suitable
color	opaque
shipped in	ambient
storage temp.	2-8°C

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
Packaging
25 μmol in poly bottle
0.1 mmol in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Thymidine 5-triphosphate sodium salt solution

CAS Number:	18423-43-3
MDL number:	MFCD00080338
PubChem Substance ID:	24900595

For DNA chain termination during PCR
For sequencing applications

assay	99%
Quality Level	200,
form	liquid
concentration	100 mM in H₂O
color	colorless
foreign activity	DNase, RNase, none detected
shipped in	dry ice
storage temp.	20°C
SMILES string	[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
InChI	1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key	KAELIXTVSANDSE-UHFFFAOYSA-N

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Titan One Tube RT-PCR Kit 11939823001

Titan™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.
The Titan One Tube RT-PCR Kit is designed for fast, sensitive and reproducible analysis of RNA by high fidelity reverse transcription polymerase chain reaction (RT-PCR) in a one-step reaction.
Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Titan One Tube RT-PCR Kit has been used:

to complete cDNA synthesis in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
to amplify full-length and truncated cDNA fragments of C-X-C motif chemokine 5 (CXCL5)
to analyze RNA (total RNA, poly (A)+ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,† then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods
for cloning of RNA up to 6 kb
for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques

Is faster than conventional two-step RT-PCR.
Packaging
1 kit containing 9 components

Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparison to Taq DNA polymerase.
Delivers increased sensitivity due to the high efficiency of all three enzymes.

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche

Quality Level	100,
usage	sufficient for 50 reactions
feature	dNTPs included: no, hotstart: no
Торговая марка	Roche
parameter	42-60 °C optimum reaction temp.
application(s)	RT-PCR: suitable
input	purified RNA
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H319 - H412
pcodes	P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

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Titan One Tube RT-PCR System 11855476001

The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:

Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
Delivers increased sensitivity due to the high efficiency of all three enzymes.
Is faster than conventional two-step RT-PCR.

Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Titan™ One Tube RT-PCR System has been used:

to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
for determining the presence or absence of RNA templates
in cloning of rare messages without the need to construct cDNA libraries
for quantifying the levels of gene expression

Features and Benefits
Titan™ One Tube RT-PCR System:

High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
offers a threefold higher fidelity in comparison to Taq DNA polymerase
delivers increased sensitivity due to the high efficiency of all three enzymes
faster than conventional two-step reverse transcriptase (RT)-PCR
allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System

Packaging
1 kit containing 4 components

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 100 reactions
feature	dNTPs included: no, hotstart: no
Торговая марка	Roche
parameter	42-60 °C optimum reaction temp.
application(s)	RT-PCR: suitable
input	purified RNA
shipped in	dry ice
storage temp.	−20°C

In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66 kDa and 51 kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.

pictograms	GHS07
signalword	Warning
hcodes	H319 - H412
pcodes	P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

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Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications:

Synthesis of first-strand cDNA for use in subsequent amplification reactions
RT-PCR of GC-rich RNA templates
Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
Retrieving and cloning the 5 and 3 termini of mRNA by RACE
Generation of cDNA libraries with large inserts
Dideoxy DNA sequencing
RNA sequencing
3-end labeling of DNA fragments
Generation of single-stranded probes for genomic footprints
In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)

Biochem/physiol Actions
Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.
Features and Benefits

Achieve high sensitivity in two-step RT-PCR.

Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler®Instruments).

Obtain more full-length transcripts - up to 14 kb.

cDNA libraries with large inserts can be generated.

Reverse transcribe difficult templates.

The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.

Efficiently label cDNA.

Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.
Components

Transcriptor Reverse Transcriptase, in storage buffer
Transcriptor RT buffer, 5x concentrated

Quality
Each lot of Transcriptor Reverse Transcriptase is routinely function tested in RT-PCR:

with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
with the LightCycler® Instrument to detect 5 x 102 to 5 x 106 copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.

Preparation Note
Volume activity: 20 U/µl.
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:

long targets, because it can transcribe up to 14 kb RNA templates
rare targets, because it has high sensitivity
GC-rich targets, because it can operate at high temperatures (up to +65oC) to eliminate problems associated with extensive secondary structure

Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.

For life science research only. Not for use in diagnostic procedures.

LightCycler is a registered trademark of Roche

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 200 reactions (03531287001), pkg of 25 reactions (03531317001), pkg of 50 reactions (03531295001)
application(s)	RT-qPCR: suitable
detection method	probe-based

TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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TRI Reagent®

MDL number:	MFCD00213058
NACRES:	NA.52

TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Easily scalable RNA isolation
Works with many sources: human, plant, yeast, bacterial, or viral
Better yields than traditional guanidine thiocyanate/cesium chloride methods

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Quality Level	100
grade	for DNA, RNA and protein isolation
usage	1 mL sufficient for 10⁷ cells, 1 mL sufficient for 100 mg tissue
impurities	phenol

pictograms	GHS05,GHS06,GHS08,GHS09
signalword	Danger
hcodes	H301 + H311 + H331 - H314 - H341 - H373 - H411
pcodes	P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Supp Hazards	EUH032
Personal Protective Equipment	Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
RIDADR	UN 2922 6.1(8) / PGIII
WGK Germany	WGK 3
Flash Point F	174.2 °F - closed cup
Flash Point C	79 °C - closed cup

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Tth DNA Polymerase 11480022001

2.7.7.7 ( BRENDA | IUBMB )

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5-3 DNA polymerase, and lacks 3-5 exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).

Thermus thermophilus (Tth) DNA Polymerase might be used:

to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
for real time PCR

Biochem/physiol Actions
Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5-3 DNA polymerase and lacks 3-5 exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb.
Features and Benefits
Tth DNA Polymerase:

Ensures optimized polymerase chain reaction (PCR) product size for at least up to 1,000 bp in a RT-PCR reaction
Accepts modified desoxyribonucleoside triphosphates as substrates
Has no association with RNase H activity
Has high thermostability to overcome the problem, typically associated with the high degree of secondary structure present in RNA

Packaging
1 kit containing 3 components
Quality
Each lot of Tth DNA Polymerase is assayed for activity on activated DNA. A function test is performed using ?DNA, as well as human genomic DNA. Performance in RT/PCR is assayed using total mouse RNA and primers specific for ?-actin. Each lot of Tth DNA Polymerase is tested for the absence of contaminating activities like exo- and endonucleases and nicking activities according to the current Quality Control procedures. A unique self-priming assay ensures the absence of contaminating DNA according to the current Quality Control procedures.
Unit Definition
One unit of Tth DNA Polymerase is defined as the amount of enzyme which catalyses the incorporation of 10 nmol total dNTPs into acid precipitable DNA within 30 minutes at +70 °C under the assay conditions stated in "unit assay".
Unit Assay: Incubation buffer for assay on activated DNA
67 mM Tris-HCI, pH 8.8 (+25 °C), 16.6 mM (NH4)2SO4, 6.7 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM dATP, dCTP, dGTP, dTTP each.
Incubation procedure
12.5 µg activated herring sperm DNA and 0.1 µCi [32P] dCTP are incubated with 0.01 to 0.1 units Tth DNA Polymerase in 50 µl Incubation buffer with a paraffin oil overlay at +70 °C for 30 min.
The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 0.5 to 5 U per reaction of PCR (optimal)
2.5 U per reaction of PCR (standard)
Determined in the assay on activated DNA described under "unit assay".

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤200 reactions
Quality Level	100,
packaging	pkg of 500 U (2 x 250_U)
Торговая марка	Roche
parameter	75 °C optimum reaction temp.
optimum pH	~9.0 (25 °C)
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Universal ProbeLibrary

Кат. номер
4684974001
4685059001
4685067001
4685075001
4685091001
4685008001
4685016001
4685024001
4685032001
4684982001

Each Universal ProbeLibrary probe is a single hydrolysis probe that recognizes sequences from one or more of the following organisms: humans, chimpanzees and other primates, mice, rats, C. elegans, Drosophila, Arabidopsis.
Short hydrolysis probes for quantification of gene expression levels by real-time PCR.

Use the Universal ProbeLibrary Probes to obtain rapid, flexible quantification of expression levels for virtually any gene from the human, mouse, rat, chimpanzee, C. elegans, Drosophila, or Arabidopsis transcriptomes through qRT-PCR or Real-time RT-PCR in the NCBI Reference Sequence Database. Each probe can be used with standard reagents in real-time PCR assays:

on the LightCycler® Carousel-Based System (e.g., with the LightCycler® TaqMan® Master), or
on other commercially available real-time PCR instruments (e.g., with the FastStart TaqMan® Probe Master).

Note: You can easily design gene-specific quantification assays using these probes and the free web-based ProbeFinder software at the Assay Design Center.
Features and Benefits
Each of the Universal ProbeLibrary probes:

Significantly reduces assay design time and implementation time.
Includes features that make probe-based assays specific, flexible, and convenient.
Needs no special hardware or unusual reaction conditions.

Components
Two vials, each containing 125 μL of one probe solution [10 μM solution of a single probe, 5′ labeled with fluorescein (FAM) and 3′ labeled with a dark quencher dye].
Quality
Function test: All Universal ProbeLibrary probes are tested for performance in real-time PCR using primers and control templates from the Universal ProbeLibrary Control Set. Each probe must pass this test.
Purity: Each probe is analyzed by anion-exchange HPLC.
Composition: Each probe is analyzed by MALDI-MS.

Probe chemistry: Universal ProbeLibrary probes use a unique nucleotide chemistry called LNA (Locked Nucleic Acid), which allows very short (8-9 bases) oligonucleotides to be effective hybridization probes in real-time PCR assays. LNA allows the melting temperature of the short probes to be unusually high. Thus, they are fully compatible with commonly used PCR conditions and the hydrolysis-probe detection format. These probes are even sensitive enough to detect single-base mismatches.
Assay design: The free online ProbeFinder software, available at the Universal ProbeLibrary Assay Design Center,, designs one or more intron-spanning assays for a target gene, based on specific information (i.e., gene name, accession number, or sequence) that you submit. For each assay it designs, the software will specify a set of specific primers plus a probe from the Universal ProbeLibrary that will give the best results. The combination of primers and probe will provide specific amplification and detection of your target sequence in a standard real-time PCR assay.
Labeling: pre-labeled with a reporter fluorophore (FAM) and a dark quencher dye.
Instrumentation required: standard real-time PCR instrumentation.
Notes: In addition to being available individually, the Universal ProbeLibrary probes are available in sets (90 probes/set), each of which covers virtually all the transcripts from a single organism. Together, the sets cover approximately 99% of the human, primate, mouse, rat, C. elegans, Drosophila, and Arabidopsis gene transcripts listed in the NCBI Reference Sequence Database.
For more information, see the listings for these organism-specific sets.
For life science research only. Not for use in diagnostic procedures.
Universal ProbeLibrary System Performance Data,
Universal ProbeLibrary System User Statements and Application,
Universal ProbeLibrary System Technology,

LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of Probe #1 (04684974001), pkg of Probe #10 (04685091001), pkg of Probe #2 (04684982001), pkg of Probe #3 (04685008001), pkg of Probe #4 (04685016001), pkg of Probe #5 (04685024001), pkg of Probe #6 (04685032001), pkg of Probe #7 (04685059001), pkg of Probe #8 (04685067001), pkg of Probe #9 (04685075001)

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash

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Uridine 5-triphosphate trisodium salt solution U1006-25UMO

MDL number:	MFCD00044310
PubChem Substance ID:	24900622
NACRES:	NA.52

For use in DNA-dependent RNA polymerase transcription
Biochem/physiol Actions
P2Y receptor agonist

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
form	liquid
concentration	100 mM in H₂O (adjusted to pH 7 with Trizma^® base)
color	colorless
foreign activity	DNase and RNase, none detected
shipped in	dry ice
storage temp.	20°C
SMILES string	[Na+].[Na+].[Na+].O[C@H]1[C@@H](O)[C@@H](O[C@@H]1COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)N2C=CC(=O)NC2=O
InChI	1S/C9H15N2O15P3.3Na/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;;/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H,10,12,15)(H2,16,17,18);;;/q;3*+1/p-3/t4-,6-,7-,8-;;;/m1.../s1
InChI key	MMJGIWFJVDOPJF-LLWADOMFSA-K
Gene Information	human ... P2RY2(5029) rat ... P2ry2(29597)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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-Amylase 10102814001

3.2.1.1 ( BRENDA | IUBMB )

α-Amylase or 1,4-α-D-glucan-glucanohydrolase is a family of starch converting endoamylase enzymes. Endoamylases cleave at the endo or internal α,1-4 glycosidic bonds present in amylose and amylopectin. This reaction results in variable length oligosaccharides such as α-dextrins, which are branched oligosaccharides.
Specificity
Hydrolyzes α1,4-glucan bonds in polysaccharides with three or more α1,4-bound glucose units.

-amylase is used for the hydrolyzation of 1,4-glycosidic bonds in polysaccharides containing 3 or more 1,4-linked D-Glucose units. The enzyme produces -dextrins.
Packaging
50mg (5 mL)
Unit Definition
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Preparation Note
Activator: NaCI (10 mM) activates the pancreatic enzyme.
Stabilizers: Both, the bacterial and the pancreatic enzymes are stabilized by Ca2+ (bound Ca2+ is required for activity).
Working concentration: 2.5 to 5 mg/ml
Working solution: Phosphate buffer (100 mmol/l; pH 7.0).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~1000 units/mg protein (At 25 °C with soluble starch as the substrate, standardized with BSA.)
mol wt	M_r 50 kDa
packaging	pkg of 5 mL
Торговая марка	Roche
optimum pH	7
shipped in	wet ice

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 - P342 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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-Gal Reporter Gene Assay, chemiluminescent 11758241001

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.

The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Features and Benefits

Sensitive: Chemiluminescence technology allows detection of approximately 20 fg -galactosidase in cell extracts
High dynamic measuring range: Linear range over four to five orders of magnitude
Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
Safe: No radioactive isotopes are used
Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Packaging
1 kit containing 7 components.
Preparation Note
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 250 assays (tubes), sufficient for 500 assays (microplate)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS05,GHS07
signalword	Danger
hcodes	H225 - H290 - H314 - H317 - H336 - H412
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 2

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-Gal Staining Set 11828673001

The -Gal Staining Set detects bacterial -Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a -Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol).
For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial -galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of -Gal as described for transfected cells.
Specificity
Bacterial β-galactosidase

The β-Gal Staining Set is used for the histochemical staining of cells and tissue sections demonstrating β-galactosidase activity.
β-Gal staining set has been used in immunofluorescence analysis, senescence assay and histopathologic analysis of tissue samples.
Packaging
1 set containing 2 components.
Specifications
Stability: Stable at +2 to +8°C

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 100 tests (in 3.5 cm dishes)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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-Galactose Dehydrogenase

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase
Specificity
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Quality
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
Sequence
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
Unit Definition
One unit galactose dehydrogenase will oxidize 1 mol of -D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
Physical form
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
packaging	pkg of 1 mL (10104973001 [1 mg]), pkg of 1 mL (10104981001 [5 mg])
Торговая марка	Roche
optimum pH	8.4 (0.1 M in Tris buffer)
pH range	4.6 - 9.2
shipped in	wet ice
storage temp.	2-8°C

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-Galactose Dehydrogenase S 10662046001

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase

β-Galactose Dehydrogenase S has been used in the colorimetric microassay method to determine the level of galactose and galactose-1-phosphate in blood.
Quality
Contaminants: <0.01% ADH, <0.01% -galactosidase, <0.1% LDH, <0.05% NADH oxidase
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)
packaging	pkg of 50 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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-Galactosidase 10105031001

3.2.1.23 ( BRENDA | IUBMB )

-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.
Specificity
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.

β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays.
Quality
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
Physical form
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
Preparation Note
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of -galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM -mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl--D-galactopyranoside.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~300 units/mg protein (At 37 °C with 2-nitrophenyl--D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.)
mol wt	540 kDa
packaging	pkg of 1,500 U
Торговая марка	Roche
optimum pH	7
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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2-Macroglobulin 10602442001

α2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.

α2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.
Biochem/physiol Actions
α2-Macroglobulin is a plasma endopeptidase and glycoprotein which functions on proteinases, especially serine proteases. It interacts with and inhibits endopeptidases. However, it does not act on other proteases, including inactive proteinases.
α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.
Sequence
2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.
Preparation Note
Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use 2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. 2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Analysis Note
Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of 2-macroglobulin is recommended.

For life science research only. Not for use in diagnostic procedures.

biological source	bovine plasma
Quality Level	100
form	lyophilized (Contains 6% BSA)
packaging	pkg of 25 IU
Торговая марка	Roche
application(s)	blocking: suitable
pH range	4 - 7
UniProt accession no.	Q7SIH1,
shipped in	wet ice
Gene Information	bovine ... A2M(513856)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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3-Hydroxybutyrate Dehydrogenase (3-HBDH)

1.1.1.30 ( BRENDA | IUBMB )

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
Quality
Contaminants: <0.1% LDH, <5% MDH
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Stabilizers: NADH, Ca2+

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
packaging	vial of 2 mL (10127833001), vial of 5 mL (10127841001)
Торговая марка	Roche
concentration	5 mg/mL
impurities	<0.1% LDH, <5% MDH
optimum pH	6.2-6.9(for reduction), 7-9(for oxidation)
shipped in	wet ice
storage temp.	2-8°C

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5-Bromo-2-deoxy-uridine Labeling and Detection Kit I 11296736001

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.
Normally, binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid, base, or heat. These procedures result in destruction of cell integrity, including cell morphology and surface and cytoplasmatic markers.
The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.
Specificity
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU. The antibody to BrdU supplied with the kit contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments, which allow binding of the antibody to BrdU without destruction of the cellular morphology. A fluorescein-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. Subsequently, the sample is evaluated using an immunofluorescence microscope.

Safe: No radioisotopes are used
Easy to perform: Follows a standard immunofluorescence protocol
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
Flexible: Allows double-labeling protocols

BrdU Labeling and Detection Kit has been used for the detection of 5-bromo-2-deoxy-uridine (BrdU) incorporated into cellular DNA.
Packaging
1 kit containing 5 components.
Preparation Note
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [3H]-thymidine, into cellular DNA, followed by autoradiography. Alternatively, 5-bromo-2-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Working solution: BrdU labeling medium
Dilute BrdU labeling reagent 1:1000 with sterile cell culture medium (final concentration 10µM).
Note: For in vivo labeling undiluted BrdU labeling reagent (1 to 2ml/100 g body weight) is needed.
Prepare shortly before use.
Anti-BrdU working solution
Dilute anti-BrdU solution 1:10 with Incubation buffer.
Prepare shortly before use.
Anti-mouse-Ig-fluorescein stock solution
Dissolve anti-mouse-Ig-fluorescein solution in 1ml double-dist. water.
Anti-mouse-Ig-fluorescein working solution
Dilute anti-mouse Ig-fluorescein stock solution 1:10 with PBS. If an extended storage is desired, add BSA (bovine serum albumin), 10 mg/ml.
Prepare shortly before use.
Washing buffer
Dilute Washing buffer concentrate (10x) (bottle 2) 1:10 with double-dist. water.
Storage conditions (working solution): BrdU labeling medium
Store undiluted (1000x) medium in aliquots at -15 to -25°C.
Anti-BrdU working solution
Store undiluted antibody at -15 to -25°C.
Anti-mouse-Ig-fluorescein stock solution
Stable at 2 to 8°C
Washing buffer
Stable at 2 to 8°C
Sample material: Cell culture: adherent cells, suspension cells, organ, or explant cultures. Tissue sections (after in vivo labeling with BrdU).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for ≤100 tests
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H317 - H319 - H412
pcodes	P261 - P264 - P273 - P280 - P333 + P313 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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5-Bromo-2-deoxy-uridine Labeling and Detection Kit II 11299964001

Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Specificity
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.
5-Bromo-2-deoxy-uridine Labeling and Detection Kit II has been used in:

labeling of tooth roots for histology
immunostaining of mice frontal sections
immunofluorescence imaging of hepatocellular carcinoma sections

Packaging
1 kit containing 7 components.
Principle
Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.
Preparation Note
Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

Safe: No radioisotopes are used.
Easy to perform: Follows a standard immunohistochemistry protocol.
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
Flexible: Allows double-labeling protocols.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for ≤100 tests
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS02,GHS07,GHS08
signalword	Danger
hcodes	H226 - H312 + H332 - H317 - H319 - H360D
pcodes	P201 - P210 - P261 - P280 - P308 + P313 - P370 + P378
WGK Germany	WGK 2
Flash Point F	136.4 °F
Flash Point C	58 °C

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5-Bromo-2-deoxy-uridine Labeling and Detection Kit III 11444611001

Proliferation in cell populations may be studied by incorporating the radioisotope [3H]-thymidine into cellular DNA. The amount of radioactive thymidine incorporated is determined by scintillation counting. Alternatively , 5-bromo-2-deoxy-uridine may be incorporated into cellular DNA. The amount of BrdU incorporated is determined by a standard ELISA protocol, which involves "tagging" the incorporated nucleotide with an anti-BrdU antibody. 96-well microplate cell ELISA for the detection of 5-bromo-2-deoxy-uridine (BrdU) incorporated into cellular DNA. Nonradioactive alternative for [3H]-thymidine based DNA synthesis and cell proliferation assays.
Contents

BrdU Labeling Reagent, 1,000x concentrated
Washing Buffer concentrate, 10x concentrated
Incubation Buffer
Nucleases
Anti-BrdU-POD, Fab fragments
Substrate Buffer
ABTS Substrate
Substrate Enhancer

Specificity
Anti-BrdU-peroxidase, Fab fragments, specifically bind to 5-bromo-2′-deoxy-uridine incorporated into DNA. It shows no cross-reactivity with any endogenous cellular components, such as thymidine or uridine.

The kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format.
5-Bromo-2-deoxy-uridine Labeling and Detection Kit III has been used in cell proliferation assay, BrdU assay and proliferation assay.
Features and Benefits

Safer, since the kit does not use radioisotopes.
Accurate, since results generated with this assay strongly correlate to those obtained with the [3 H]-thymidine method. (See Figure 2 below.)
Sensitive. This assay and the [3 H]-thymidine assay are equally sensitive. (See Figure 2 below.)
Fast, since results can be read with a multi-well ELISA reader, allowing simultaneous processsing of a large number of samples
Easy, since the assay uses a standard cell ELISA protocol.
Economical, since the assay requires no expensive equipment or additional reagents (e.g ., scintillation fluid).

Packaging
1 kit containing 8 components.
Principle
Cells cultured in a 96-well microplate are incubated with BrdU (see Performance). The labeled cells are fixed with ethanol. Prior to incubation with a monoclonal antibody to BrdU, DNA is partially digested with nucleases to allow the antibody to access BrdU. Next, the anti-BrdU antibody [labeled with peroxidase (POD)] is added. Finally, the POD substrate ABTS is added. POD catalyzes the cleavage of ABTS, producing a colored reaction product. The absorbance of the samples (at approximately 405 nm) is determined with a standard microplate (ELISA) reader.
Preparation Note
Working solution: BrdU labeling solution

Dilute BrdU labeling reagent 1 : 90 with sterile PBS or culture medium (resulting concentration: 111 µM BrdU) [e.g., for one 96-well microplate containing 100 µl medium per well, dilute 12 µl BrdU labeling reagent with 1.068 ml sterile PBS].
Note: The BrdU labeling solution should be prepared freshly before use.

Washing buffer
Dilute washing buffer concentrate (10x) 1 : 10 with double-dist. water [e.g., for one 96-well microplate dilute 9 ml washing buffer concentrate (10x) with 81 ml double-dist. water].
Note: If precipitates in Washing buffer, 10x conc. are visible, please incubate the bottle for 10 minutes at 37 °C in a water bath before you prepare Solution II.
Washing buffer is used to:

Prepare the anti-BrdU-peroxidase, working solution
Wash cells after incubation with anti-BrdU-peroxidase

Note: For all other washing steps use PBS or culture medium containing 10% serum [e.g., FCS (fetal calf serum)] to obtain reliable results.
Incubation buffer

Ready-to-use
Used to dilute the nucleases

Nucleases, stock solution
Reconstitute the nucleases in 1.3 ml double-dist. water containing 50% glycerol (w/v).
Nucleases, working solution
Dilute nucleases, stock solution, 1 : 100 with incubation buffer (e.g., for one 96-well microplate dilute 100 µl nucleases, stock solution, with 9.9 ml incubation buffer).
Anti-BrdU-peroxidase, Fab fragments, stock solution

Dissolve anti-BrdU-peroxidase, Fab fragments in 1.25 ml double-dist. water (final concentration: 20 U/ml).
Anti-BrdU-peroxidase, Fab fragments, working solution
Prepare anti-BrdU-peroxidase, Fab fragments, working solution shortly before use. Dilute anti-BrdU-peroxidase, Fab fragments, stock solution 1 : 100 with washing buffer supplemented with 10 mg/ml BSA (bovine serum albumin), [e.g., for one 96-well microplate dilute 100 µl anti-BrdU-peroxidase, Fab fragments, stock solution, with 9.9 ml PBS and BSA (final concentration: 200 mU/ml)].
Peroxidase substrate
Dissolve the ABTS powder in substrate buffer and stir at 15 to 25 °C to obtain a clear solution.
Peroxidase substrate containing substrate enhancer
If a low signal is expected, take an appropriate aliquot of substrate solution and add substrate enhancer , 1 mg/ml and dissolve by stirring for 15 minutes at 15 to 25 °C (e.g., for one 96-well microplate dissolve 10 mg substrate enhancer in 10 ml peroxidase substrate)
Note: The substrate solution containing substrate enhancer is stable for only 4 hours and should, therefore, be freshly prepared before use.
Storage conditions (working solution): BrdU labeling reagent
The undiluted BrdU labeling reagent (1000x) is stable at 2 to 8 °C for 6 months. It is stable stored in aliquots at -15 to -25 °C.
Washing buffer
Stable at 2 to 8 °C for 3 months.
Incubation buffer
Stable at 2 to 8 °C until the expiration date printed on the label
Nucleases, stock solution
Stable at -15 to -25 °C for 6 months.
Nucleases, working solution
must be prepared shortly before use.
Anti-BrdU-Peroxidase, Fab fragments, stock solution
Stable at 2 to 8 °C for 6 months. For long term storage it is recommended to store the solution in aliquots at -15 to -25 °C.
Anti-BrdU-Peroxidase, Fab fragments, working solution
must be prepared freshly before use.
Peroxidase substrate
Stable at 2 to 8 °C for 2 months when stored protected from light.
Peroxidase substrate containing substrate enhancer
must be prepared freshly before use

For life science research only. Not for use in diagnostic procedures.

P261 - P273 - P280 - P333 + P313 - P337 + P313 - P362 + P364

usage	sufficient for ≤1,000 tests
Quality Level	100
specific activity	10000 (Nuclease activity (vial 4))
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

5-Bromo-2-deoxyuridine 10280879001

Empirical Formula (Hill Notation):	C₉H₁₁BrN₂O₅
CAS Number:	59-14-3
Molecular Weight:	307.10
Beilstein/REAXYS Number:	30395
MDL number:	MFCD00006529
PubChem Substance ID:	329749004

It is conventionally used as a marker for S-phase synthesis of DNA.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Biochem/physiol Actions
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
Quality
Purity: 97% (from N), chromatographically homogeneous
Preparation Note
Working concentration: According to literature for cell culture, a final concentration of 10 µmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

For life science research only. Not for use in diagnostic procedures.

assay	>97%
Quality Level	100,
mol wt	307.1
packaging	pkg of 1 g
Торговая марка	Roche
mp	191-194 °C (dec.) (lit.)
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI	1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key	WOVKYSAHUYNSMH-RRKCRQDMSA-N

pictograms	GHS08
signalword	Warning
hcodes	H351
pcodes	P201 - P202 - P280 - P308 + P313 - P405 - P501
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable

5/3 RACE Kit, 2nd Generation 3353621001

Rapid Amplification of cDNA ends (RACE) is a prevalent technique used to rapidly obtain full-length cDNA from partially known sequences. The 5/3 RACE kit contains Transcriptor Reverse Transcriptase and recombinant Terminal Transferase. Transcriptor Reverse Transcriptase transcribes full-length cDNA for the highly sensitive and rapid amplification of either 5 or 3 cDNA fragments up to 14 kb. Due to its thermostability (up to +65 °C), it can work with GC-rich templates with high secondary structure. High sensitivity can be achieved using Transcriptor Reverse Transcriptase, resulting in highly efficient cDNA synthesis and the generation of long RACE products. Recombinant Terminal transferase is used to add a homopolymeric A-tail to the 3 end of the cDNA. The poly(A)+ tail decreases the likelihood of inappropriate truncation by the oligo(dT)-anchor primer, and overcomes the weaker A/T compared to the G/C hybridization. Moreover, longer stretches of A residues are required before the oligo(dT)-anchor primer can hybridize to an internal site and can truncate the amplification product. Tailed cDNA is amplified by PCR using a gene-specific primer and the oligo(dT)-anchor primer. The obtained cDNA is further amplified by a second PCR using a nested specific primer and the PCR-anchor primer, allowing RACE products to be cloned into an appropriate vector for subsequent studies.
Specificity
Heat inactivation: Terminal Transferase: 70 °C for 10 minutes
Transcriptor Reverse Transcriptase: 85 °C for 5 minutes

5/3 RACE Kit, 2nd Generation is suitable for

Structural and expression studies of RNA molecules
Generating full-length cDNAs
Isolation and characterization of 5 or 3 ends from low-copy RNA messages
first strand cDNA synthesis
Amplification and further cloning of rare mRNAs
Use in conjunction with exon-trapping methods
Products of the RACE reaction can be directly sequenced without cloning

Features and Benefits
The control reaction includes transcription of the control RNA into first-strand cDNA using the control primer neo1/rev. Amplification of the cDNA is performed using the control primer neo2/rev and neo3/for to obtain a 157-bp PCR product. Tailing of the purified cDNA is performed with dATP. Amplification of the tailed cDNA is performed with oligo dT-anchor primer and the control primer neo2/rev to obtain a 293-bp PCR product.

Robust performance: Recombinant Transcriptor Reverse Transcriptase allows procession through regions of difficult secondary RNA structure.
Convenient: Function and expression studies of either 5 or 3 end of the RNA can be performed with the same kit.
Reliable: dA tailing of cDNA with Recombinant Terminal Transferase decreases the likelihood of inappropriate truncation.
Reproducible: Oligo dT-anchor primer with non 3dT ensures correct binding to the inner end of the poly (A) tail.
Produce long fragments: Generate cDNA up to 14kb in length with Transcriptor Reverse Transcriptase.

Packaging
1 kit containing 12 components

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 10 reactions
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

7-Deaza-2-deoxy-guanosine-5-triphosphate 10988537001

Empirical Formula (Hill Notation):	C₁₁H₁₇N₄O₁₃P₃
CAS Number:	101515-08-6
Molecular Weight:	506.19
MDL number:	MFCD00171614
PubChem Substance ID:	329749263

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
Preparation Note
Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.
Analysis Note
Absorbance determination: A250/A260, A280/A260, A290/A260
Absorption: 25 A260 units of product correspond to 1 mg.

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	≤4% (7-Deaza-dGDP, HPLC), 95% (7-Deaza-dGTP, HPLC)
form	solution
packaging	pkg of 200 μL (10 mM, 2 μmol)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C
SMILES string	NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI	1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key	DLLXAZJTLIUPAI-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

ABTS™ 10102946001

Empirical Formula (Hill Notation):	C₁₈H₂₄N₆O₆S₄
CAS Number:	30931-67-0
Molecular Weight:	548.68
Beilstein/REAXYS Number:	7329461
MDL number:	MFCD00010404
PubChem Substance ID:	329748893

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

ABTS (C18H16N4O6S4-(NH4)2) is a peroxidase substrate for ELISA.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Quality
Purity: >98% (absorbance), chromatographically homogeneous
Preparation Note
Working concentration: ABTS should be dissolved as follows:

100 mg ABTS in in 100ml 3.25 mM sodium perborate, 39.8 mM citric acid, 60 mM disodium hydrogen phosphate, pH 4.4-4.5 (see also PI of SA-peroxidase). This buffer corresponds to the single reagent ABTS-Buffer, Cat. No. 1204530. The formed product is green and soluble in water. Measurement at 405 nm.
Dilution of ABTS:
ABTS/ buffer solution (9.1 mM ABTS; pH 5.0): 99,9 mg ABTS in 20 ml potassium phosphate buffer (0.1M; pH 5.0).
Storage and Stability
Store at 2 to 8 °C. (It is also possible to store at -15 to -25 °C)

For life science research only. Not for use in diagnostic procedures.

ABTS is a trademark of Roche

assay	>98%
Quality Level	100,
form	crystalline
mol wt	548.68
packaging	pkg of 2 g
Торговая марка	Roche
solubility	50 mg/mL
_max	414 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI	1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key	OHDRQQURAXLVGJ-AXMZSLBLSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

ABTS™ Buffer 11204530001

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Note
Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

For life science research only. Not for use in diagnostic procedures.

ABTS is a trademark of Roche

form	solution
Quality Level	100,
packaging	pkg of 125 mL
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available