MILLIPLEX® Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay

eCl@ss:

32161000

Кат. номер

HNS2MAG-95K-01

HNS2MAG-95K-02

HNS2MAG-95K-03

HNS2MAG-95K

HNS2MAG-95K-05

HNS2MAG-95K-06

HNS2MAG-95K-07

HNS2MAG-95K-04

Monitoring protein biomarkers in cerebrospinal fluid (CSF) of patients with neurological disorders has been highly beneficial to understanding disease progression. While several CSF biomarkers can reproducibly distinguish normal and diseased samples, CSF is a difficult biological fluid to obtain in research studies. The need for blood-based biomarkers of AD has driven a continuous search for novel candidates. This configurable multiplex immunoassay quantitatively measures six proteins present in both human CSF and blood plasma/serum that are involved in neurological disease. Cell/tissue culture samples may also be used in the panel.
MILLIPLEX® Human Neuroscience Bead Panel 2 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF), serum/plasma and cell/tissue culture supernatants for homogenates: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, TREM2.
Panel Type: Neuroscience
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. There is <5% cross-reactivity from ApoE2 and ApoE3 recombinant proteins on the ApoE4 beads.

• Analytes: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, TREM2
• Recommended Sample type: Plasma, Serum, CSF, Cell/Tissue culture supernatant
• For Plasma/Serum a 1:10 dilution is recommended for the optimal detection of the analytes FABP3, Ferritin, Neurogranin and TREM2.
• For Plasma/Serum a 1:200 dilution for ApoE4 and Angiogenin is recommended.
• Because the sample dilutions differ for Plasma/Serum samples ApoE4 and Angiogenin cannot be plexed in the same assay as FABP3, Ferritin, Neurogranin and TREM2.
• CSF samples require a 1:10 dilution and so may all be plexed together
• Cell/Tissue culture supernatants or homogenates may be used neat or diluted in appropriate control medium.
• Bead diluent must be present in order to detect Angiogenin. (Biotinylated-Angiogenin detection antibody is included in the bead diluent.)
• Research Category: Neuroscience

Design your multiplex kit by choosing available analytes within this panel.
Everything you need in a single kit.
Recommended storage for kit components is 2 - 8°C.
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

species reactivity	human
Торговая марка	Milliplex®
assay range	accuracy: 124% (Neurogranin), accuracy: 84% (FABP3), accuracy: 85% (ApoE4), accuracy: 94% (TREM2), accuracy: 97% (Angiogenin), sensitivity: 11.0 pg/mL (MinDC + 2SD; TREM2), sensitivity: 14.3 pg/mL (MinDC + 2SD; FABP3), sensitivity: 162.8 pg/mL (MinDC + 2SD; ApoE4), sensitivity: 19.9 pg/mL (MinDC + 2SD; Neurogranin), sensitivity: 5.8 pg/mL (MinDC + 2SD; Angiogenin), sensitivity: 6.1 pg/mL (MinDC + 2SD; Ferritin), standard curve range: 15-50,000 pg/mL (TREM2), standard curve range: 2-10,000 pg/mL (Angiogenin), standard curve range: 24-100,000 pg/mL (FABP3), standard curve range: 244-1,000,000 pg/mL (ApoE4), standard curve range: 5-20,000 pg/mL (Neurogranin), standard curve range: 6-25,000 pg/mL (Ferritin), inter-assay cv: <10% intra-assay cv: <5% (Angiogenin), inter-assay cv: <10% intra-assay cv: <5% (FABP3), inter-assay cv: <10% intra-assay cv: <5% (TREM2), inter-assay cv: <15% intra-assay cv: <10% (Ferritin), inter-assay cv: <15% intra-assay cv: <10% (Neurogranin), inter-assay cv: <15% intra-assay cv: <5% (ApoE4)
application(s)	multiplexing: suitable
detection method	fluorometric (Luminex xMAP)