Lipopolysaccharides from Escherichia coli O127:B8

EC Number:	297-473-0
MDL number:	MFCD00212998
NACRES:	NA.25

Кат. номер

L3129-100MG-PW

L3129-10MG-PW

L3129-25MG

L3129-100MG

L3129-10MG

L3129-25MG-PW

This product is phenol extracted from E. coli serotype O127:B8. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.
10 mg in poly bottle
25, 100 mg in glass bottle
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Quality Level	200
biological source	Escherichia coli (O127:B8)
form	lyophilized powder
purified by	phenol extraction
impurities	<3% Protein (Lowry)
shipped in	ambient
storage temp.	2-8°C

pictograms	GHS06
signalword	Danger
hcodes	H300
pcodes	P264 - P270 - P301 + P310 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable