GenElute™ Bacterial Genomic DNA Kits NA2120-1KT

NACRES:

NA.55

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

GenElute™ Bacterial Genomic DNA Kits has been used:

• to isolate DNA
• to prepare DNA from E. coli strain
• to extract genomic DNA

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

Features and Benefits

• Starting material: Up to 1.5 mL of culture
• Expected yield: Up to 20 µg
• Elution volume: 400 µl
• Time required: 70 - 120 min
• A260/A280 ratio: 1.6 - 1.9
• No phenol, chloroform, or ethanol precipitation required

Principle
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 µL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
usage	sufficient for 350 purifications
storage temp.	room temp

pictograms	GHS02,GHS05,GHS07,GHS08,GHS09
signalword	Danger
hcodes	H226 - H290 - H302 - H315 - H318 - H334 - H336 - H410
pcodes	P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	77.0 °F - closed cup
Flash Point C	25 °C - closed cup